Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,238,562 (T)7→6 intergenic (+265/‑18) ygjR → / → alx putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,238,5560T.100.0% 252.8 / NA 60intergenic (+259/‑24)ygjR/alxputative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/32);  total (28/32)

TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NC_000913/3238418‑3238690
                                                                                                                                          |                                                                                                                                      
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGCCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                        >  2:917472/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                        >  2:35717/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                        >  2:195225/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                        >  1:996719/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTCAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                                        >  2:943115/1‑139 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   <  2:790311/139‑1 (MQ=255)
       aaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                                 <  2:829948/139‑3 (MQ=255)
          cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTAtgtg                                                                                                                             >  1:570107/1‑139 (MQ=255)
            caTCCGGTGACCGGGCAACCCGAAATTAATACGCAGACGTATTCCTTTCTTGTTGTAAGTGAGAACTGGCCGTGAGGCGAGCTCTATGCATAAAAAGCTGCGGCTGACGTCTTCCGACGTAGGTCGTTTTTTATGTGTa                                                                                                                           <  2:1296429/139‑1 (MQ=255)
                 ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  1:131028/139‑1 (MQ=255)
                 ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  2:649497/139‑1 (MQ=255)
                  gTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAAc                                                                                                                     >  1:795004/1‑139 (MQ=255)
                   tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                    <  2:795004/139‑1 (MQ=255)
                           cAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGaa                                                                                                            <  2:996719/139‑1 (MQ=255)
                             aCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   <  2:319733/115‑1 (MQ=255)
                             aCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   >  1:319733/1‑115 (MQ=255)
                             aCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATa                                                                                                          >  1:18104/1‑139 (MQ=255)
                                      gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  2:42919/139‑1 (MQ=255)
                                      gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  1:17402/139‑1 (MQ=255)
                                        aTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACg                                                                                               <  2:18104/139‑1 (MQ=255)
                                        aTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACg                                                                                               >  2:666020/1‑139 (MQ=255)
                                         tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                                                                                                >  1:475048/1‑137 (MQ=255)
                                         tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                                                                                                <  2:475048/137‑1 (MQ=255)
                                         tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                              >  1:228324/1‑139 (MQ=255)
                                           cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  1:143905/139‑1 (MQ=255)
                                                      tCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg                                                                                 <  1:822855/139‑1 (MQ=255)
                                                          tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCTGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                    <  2:919253/100‑1 (MQ=255)
                                                          tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                                    >  1:919253/1‑100 (MQ=255)
                                                          tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                             >  2:1080150/1‑139 (MQ=255)
                                                                     aGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgt                                                                  >  1:916835/1‑139 (MQ=255)
                                                                        gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                               <  1:29439/139‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  1:96674/77‑1 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      >  2:96674/1‑77 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTa                                                       >  2:851933/1‑139 (MQ=255)
                                                                                 ccGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTc                                                                                                                  <  1:195670/79‑1 (MQ=255)
                                                                                 ccGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTc                                                                                                                  >  2:195670/1‑79 (MQ=255)
                                                                                                  tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttt                                                                                                                                    <  2:579459/45‑1 (MQ=255)
                                                                                                  tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttt                                                                                                                                    >  1:579459/1‑45 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   <  1:579821/41‑1 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                                   >  2:579821/1‑41 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTc                                                                                                                  >  1:1031432/1‑57 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTc                                                                                                                  <  2:1031432/57‑1 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                                >  1:1273363/1‑139 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTAt                                             <  2:974119/125‑1 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTAt                                             >  1:974119/1‑125 (MQ=255)
                                                                                                            gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            >  1:435553/1‑74 (MQ=255)
                                                                                                            gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  2:435553/74‑1 (MQ=255)
                                                                                                             cagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAgggg                          <  1:35717/139‑1 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  2:911600/67‑1 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 >  1:911600/1‑67 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 >  1:68947/1‑67 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  2:68947/67‑1 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                                                                                                >  1:660969/1‑68 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                                                                                                <  2:660969/68‑1 (MQ=255)
                                                                                                              agcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGc                         >  1:264351/1‑139 (MQ=255)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                           <  2:91870/40‑1 (MQ=38)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                           >  1:91870/1‑40 (MQ=38)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAgg                                                                                                                        <  1:232536/43‑1 (MQ=38)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAgg                                                                                                                        >  2:232536/1‑43 (MQ=38)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      <  1:673703/45‑1 (MQ=38)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                                      >  2:673703/1‑45 (MQ=38)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCt                                       >  2:636135/1‑123 (MQ=255)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCt                                       <  1:636135/123‑1 (MQ=255)
                                                                                                                   cTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcgtcgt                    <  2:780602/139‑1 (MQ=255)
                                                                                                                         tCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  1:666020/139‑1 (MQ=255)
                                                                                                                         tCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  2:264351/139‑1 (MQ=255)
                                                                                                                                 cGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  1:943115/139‑1 (MQ=255)
                                                                                                                                 cGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  1:1080150/139‑1 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                   >  2:830370/1‑90 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                   <  1:830370/90‑1 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  >  1:941410/1‑139 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  >  2:66359/1‑139 (MQ=255)
                                                                                                                                     ggCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGACGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                           >  1:75915/1‑114 (MQ=255)
                                                                                                                                          |                                                                                                                                      
TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NC_000913/3238418‑3238690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: