Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 34,120 (T)9→8 intergenic (+82/‑180) carB → / → caiF carbamoyl‑phosphate synthase large subunit/cai operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091334,1120T.98.8% 360.7 / ‑2.6 86intergenic (+74/‑188)carB/caiFcarbamoyl‑phosphate synthase large subunit/cai operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (45/40);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAA  >  NC_000913/33981‑34246
                                                                                                                                   |                                                                                                                                      
tGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGtttttttt                                                                                                                                 <  1:469350/139‑1 (MQ=255)
    gATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttttttttgccc                                                                                                                             <  2:537204/139‑5 (MQ=255)
     aTGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCa                                                                                                                           >  2:622941/1‑139 (MQ=255)
             gAAAAAGTAATTTCGGTGCAGGAAATACACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTaa                                                                                                                   <  2:693960/139‑1 (MQ=255)
                     aaTTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTg                                                                                                           <  2:1172445/139‑1 (MQ=255)
                                                 aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGc                                                                               >  2:816824/1‑139 (MQ=255)
                                                 aTCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGc                                                                               >  1:1221954/1‑139 (MQ=255)
                                                         ataGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTa                                                                       >  2:1030034/1‑139 (MQ=255)
                                                           aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCa                                                                                                                           <  1:922249/85‑1 (MQ=255)
                                                           aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCa                                                                                                                           >  2:922249/1‑85 (MQ=255)
                                                           aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTaa                                                                                                                   <  2:1100890/93‑1 (MQ=255)
                                                           aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTaa                                                                                                                   >  1:1100890/1‑93 (MQ=255)
                                                           aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGTCATAAGCAGGATTTAGc                                                                     >  1:767521/1‑139 (MQ=255)
                                                           aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGc                                                                     >  1:900738/1‑139 (MQ=255)
                                                           aGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGc                                                                     >  2:307356/1‑139 (MQ=255)
                                                             cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttttttt                                                                                                                                  >  1:654825/1‑77 (MQ=255)
                                                             cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGttttttt                                                                                                                                  <  2:654825/77‑1 (MQ=255)
                                                             cGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTc                                                                   >  1:1196135/1‑139 (MQ=255)
                                                              gtgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  <  1:1030034/139‑1 (MQ=255)
                                                              gtgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  <  2:1196135/139‑1 (MQ=255)
                                                               tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt                                                                         >  2:728370/1‑131 (MQ=255)
                                                               tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAtt                                                                         <  1:728370/131‑1 (MQ=255)
                                                               tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                          <  2:65988/130‑1 (MQ=255)
                                                               tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                          >  1:65988/1‑130 (MQ=255)
                                                               tgtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTcac                                                                 >  1:41175/1‑139 (MQ=255)
                                                                gtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGtttttttt                                                                                                                                 <  2:222214/75‑1 (MQ=255)
                                                                gtCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGtttttttt                                                                                                                                 >  1:222214/1‑75 (MQ=255)
                                                                 tCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCacac                                                               >  2:414276/1‑139 (MQ=255)
                                                                         gATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAACCAGGATTTAGCTCACACTTATCGAc                                                       >  1:1121258/1‑139 (MQ=255)
                                                                          atatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACg                                                      >  1:159287/1‑139 (MQ=255)
                                                                           tatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                              >  2:1257806/1‑114 (MQ=255)
                                                                           tatTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCa                                                                              <  1:1257806/114‑1 (MQ=255)
                                                                             tttttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                   <  1:816824/139‑1 (MQ=255)
                                                                             tttttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                   <  2:1121258/139‑1 (MQ=255)
                                                                               tttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGaa                                                 >  1:1022959/1‑139 (MQ=255)
                                                                                ttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTa                                                                                                                    <  1:665054/71‑1 (MQ=255)
                                                                                ttCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTa                                                                                                                    >  2:665054/1‑71 (MQ=255)
                                                                                     ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  <  2:1170182/116‑1 (MQ=255)
                                                                                     ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  >  1:1170182/1‑116 (MQ=255)
                                                                                     ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           <  1:622941/139‑1 (MQ=255)
                                                                                     ccGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           <  2:1022959/139‑1 (MQ=255)
                                                                                      cGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCAt                                          <  2:666765/139‑1 (MQ=255)
                                                                                        cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                >  2:796945/1‑131 (MQ=255)
                                                                                        cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           >  2:377094/1‑136 (MQ=255)
                                                                                        cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           <  1:377094/136‑1 (MQ=255)
                                                                                        cTAATTTGATCGAATAACTAATACGGTTCTCTGATGAAGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                <  1:796945/131‑1 (MQ=255)
                                                                                          aaTTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTa                                      <  2:41175/139‑1 (MQ=255)
                                                                                                aTCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGata                                >  2:137247/1‑139 (MQ=255)
                                                                                                     aTAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           >  2:321632/1‑123 (MQ=255)
                                                                                                     aTAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           <  1:321632/123‑1 (MQ=255)
                                                                                                      tAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCGTTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATcta                          <  2:776537/139‑3 (MQ=255)
                                                                                                      tAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCca                          <  1:886883/139‑1 (MQ=255)
                                                                                                       aaCTAATACGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCcac                         <  1:307356/139‑1 (MQ=255)
                                                                                                              aCGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                          >  2:860030/1‑83 (MQ=255)
                                                                                                              aCGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGAt                                                                          <  1:860030/83‑1 (MQ=255)
                                                                                                               cGGTTCTCTGATGAGGACCGTTTTTTTTTGCACTTTAAATAAATATTTTGGGGAATCGATTTTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCAGAGGAGAAGTTGTATACTATAGATATATCCACAAtttaa                  >  2:1071267/1‑137 (MQ=255)
                                                                                                               cGGTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTaa                 >  2:869636/1‑139 (MQ=255)
                                                                                                                 gTTCTCTGATGAGGACCGTTTTTTTTGTCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAata               <  2:269781/139‑1 (MQ=255)
                                                                                                                 gTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           <  2:930759/111‑1 (MQ=255)
                                                                                                                 gTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           >  1:930759/1‑111 (MQ=255)
                                                                                                                 gTTCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAata               <  2:64390/139‑1 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGAc                                                                                     >  2:123336/1‑68 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGAc                                                                                     <  1:123336/68‑1 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  >  2:63256/1‑87 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  <  1:63256/87‑1 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                   <  2:990937/102‑1 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTg                                                   >  1:990937/1‑102 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                >  1:865415/1‑105 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           >  2:617341/1‑110 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCa                                           <  1:617341/110‑1 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAatat              >  1:1231958/1‑139 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAatat              >  1:403489/1‑139 (MQ=255)
                                                                                                                  ttCTCTGATGAGGACCGGTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACATTTATCGACGGTGAAg                                                <  2:865415/105‑1 (MQ=255)
                                                                                                                    ctctGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACg                                                      >  1:926182/1‑97 (MQ=255)
                                                                                                                    ctctGATGAGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACg                                                      <  2:926182/97‑1 (MQ=255)
                                                                                                                         atgaGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  >  2:1217520/1‑80 (MQ=255)
                                                                                                                         atgaGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTca                                                                  <  1:1217520/80‑1 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTtgat                                                                                        >  1:610886/1‑55 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTtgat                                                                                        <  2:610886/55‑1 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                >  2:527528/1‑95 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                <  1:527528/95‑1 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATa                                         <  1:32548/102‑1 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATa                                         >  2:32548/1‑102 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGcc          >  2:1164824/1‑133 (MQ=255)
                                                                                                                            aGGACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGcc          <  1:1164824/133‑1 (MQ=255)
                                                                                                                              gACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                <  2:538335/93‑1 (MQ=255)
                                                                                                                              gACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                <  1:342590/93‑1 (MQ=255)
                                                                                                                              gACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                >  1:538335/1‑93 (MQ=255)
                                                                                                                              gACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAg                                                >  2:342590/1‑93 (MQ=255)
                                                                                                                              gACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTaa  >  2:1250050/1‑139 (MQ=255)
                                                                                                                              gACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTaa  >  2:167910/1‑139 (MQ=255)
                                                                                                                              gACCGTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCATTGTTTaa  >  1:361268/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                      
TGCCGATGCGACTGAAAAAGTAATTTCGGTGCAGGAAATGCACGCACAGATCAAATAATAGCGTGTCATGGCAGATATTTTTCATCCGCTAATTTGATCGAATAACTAATACGGTTCTCTGATGAGGACCGTTTTTTTTTGCCCATTAAGTAAATCTTTTGGGGAATCGATATTTTTGATGACATAAGCAGGATTTAGCTCACACTTATCGACGGTGAAGTTGCATACTATCGATATATCCACAATTTTAATATGGCCTTGTTTAA  >  NC_000913/33981‑34246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: