Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,159,059 (A)5→4 coding (570/918 nt) oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,159,0550A.97.9% 394.2 / 0.8 96coding (566/918 nt)oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (47/47);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAG  >  NC_000913/4158917‑4159189
                                                                                                                                          |                                                                                                                                      
gCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGa                                                                                                                                        <  2:1085924/139‑1 (MQ=255)
 cGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaa                                                                                                                                       >  1:117706/1‑139 (MQ=255)
   tGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGTCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaaa                                                                                                                                     >  1:236197/1‑139 (MQ=255)
        cTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgct                                                                                                                               >  1:895018/1‑139 (MQ=255)
        cTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgct                                                                                                                               >  1:88118/1‑139 (MQ=255)
        cTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAGGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgct                                                                                                                               <  2:529415/139‑1 (MQ=255)
           gcgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGAt                                                                                                                            >  1:996204/1‑139 (MQ=255)
              cTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgct                                                                                                                               >  2:219720/1‑133 (MQ=255)
              cTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGAGCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgct                                                                                                                               <  1:219720/133‑1 (MQ=255)
                ggTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTgg                                                                                                                       >  2:528004/1‑139 (MQ=255)
                  tGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATGACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGATAACTGCTGATGCTGGaa                                                                                                                     <  1:807885/139‑1 (MQ=255)
                  tGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGaa                                                                                                                     <  2:910494/139‑1 (MQ=255)
                    aaaGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGa                                                                                                                   <  1:815696/139‑1 (MQ=255)
                         gagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTc                                                                                                              <  2:1245737/139‑1 (MQ=255)
                           gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCAc                                                                                                            >  1:767780/1‑139 (MQ=255)
                           gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCAc                                                                                                            >  1:906183/1‑139 (MQ=255)
                            cGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACt                                                                                                           >  2:1182042/1‑139 (MQ=255)
                                   ttcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                                                                                    <  2:767780/139‑1 (MQ=255)
                                   ttcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                                                                                    <  1:923229/139‑1 (MQ=255)
                                       ttGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAGACTGCTGATGCTGCAATATGGTCACTATTTGCGCGAt                                                                                                <  2:861603/139‑1 (MQ=255)
                                              gCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                         <  1:552369/139‑1 (MQ=255)
                                               ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAt                                                                                        >  2:373267/1‑139 (MQ=255)
                                               ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAt                                                                                        >  1:1193891/1‑139 (MQ=255)
                                               ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAt                                                                                        >  2:588327/1‑139 (MQ=255)
                                                   tgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGt                                                                                    <  1:503724/139‑1 (MQ=255)
                                                       tgatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCt                                                                                <  1:1182042/139‑1 (MQ=255)
                                                       tgatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCt                                                                                >  2:14603/1‑139 (MQ=255)
                                                          tgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt                                                                             >  2:405873/1‑139 (MQ=255)
                                                            aGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGACGCTGGAAGATGGTCTCTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                           <  2:836912/139‑1 (MQ=255)
                                                              ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGa                                                                         >  2:178403/1‑139 (MQ=255)
                                                              ccAATGTTGCTGGCTTCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCCGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa                                                                        >  2:698966/1‑139 (MQ=255)
                                                                       cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                  >  2:125666/1‑137 (MQ=255)
                                                                       cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                  <  1:125666/137‑1 (MQ=255)
                                                                       cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                >  1:116996/1‑139 (MQ=255)
                                                                          gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                         >  2:202473/1‑111 (MQ=255)
                                                                          gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                         <  1:202473/111‑1 (MQ=255)
                                                                          gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                             <  2:1193891/139‑1 (MQ=255)
                                                                            tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 <  1:740811/133‑1 (MQ=255)
                                                                            tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 <  1:367832/133‑1 (MQ=255)
                                                                            tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 >  2:367832/1‑133 (MQ=255)
                                                                            tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                           >  2:1203100/1‑139 (MQ=255)
                                                                            tatctatGAAGATCACCAGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTAATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 >  2:740811/1‑133 (MQ=255)
                                                                              tctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgctg                                                                                                                              >  1:178988/1‑70 (MQ=255)
                                                                              tctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgctg                                                                                                                              <  2:178988/70‑1 (MQ=255)
                                                                              tctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTATCTGTTTTGAAGCCGGGGCGGATGaa                                                         >  1:247226/1‑139 (MQ=255)
                                                                                      gATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                <  1:696895/92‑1 (MQ=255)
                                                                                      gATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                >  2:696895/1‑92 (MQ=255)
                                                                                      gATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacaca                                                 >  1:1242024/1‑139 (MQ=255)
                                                                                        tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaaactg                                                                                                                                  >  2:691616/1‑54 (MQ=255)
                                                                                        tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaaactg                                                                                                                                  <  1:691616/57‑4 (MQ=255)
                                                                                        tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAt                                                                                        >  1:1140305/1‑98 (MQ=255)
                                                                                        tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAt                                                                                        <  2:1140305/98‑1 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgct                                                                                                                               <  2:675491/58‑1 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgct                                                                                                                               >  1:675491/1‑58 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                <  1:1002386/89‑1 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                >  2:1002386/1‑89 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 >  1:63417/1‑120 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 <  2:63417/120‑1 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                           >  1:558066/1‑126 (MQ=255)
                                                                                         cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                           <  2:558066/126‑1 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaa                                                                                                                                       <  2:683866/50‑1 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaa                                                                                                                                       >  1:683866/1‑50 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCa                                                                                          >  1:322970/1‑94 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCa                                                                                          <  2:322970/94‑1 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATggg                                                                                     >  1:266235/1‑99 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATggg                                                                                     <  2:266235/99‑1 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                             >  1:337943/1‑139 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                             >  1:254721/1‑139 (MQ=255)
                                                                                          aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                             >  2:1158875/1‑139 (MQ=255)
                                                                                            ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATac                                                    <  1:37627/130‑1 (MQ=255)
                                                                                            ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATac                                                    >  2:37627/1‑130 (MQ=255)
                                                                                               tGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgctg                                                                                                                              >  1:279448/1‑53 (MQ=255)
                                                                                               tGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAActgctg                                                                                                                              <  2:279448/53‑1 (MQ=255)
                                                                                                 ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa                                      <  1:14603/139‑1 (MQ=255)
                                                                                                   cgtaCCGCGAAGGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGAc                                     >  2:749472/4‑139 (MQ=255)
                                                                                                     aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAg                                  <  1:405873/139‑1 (MQ=255)
                                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                                                                                    >  2:336273/1‑72 (MQ=255)
                                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgcg                                                                                                    <  1:336273/72‑1 (MQ=255)
                                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 <  2:257339/107‑1 (MQ=255)
                                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 >  1:257339/1‑107 (MQ=255)
                                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAg                                                        <  2:530118/116‑1 (MQ=255)
                                                                                                      aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAg                                                        >  1:530118/1‑116 (MQ=255)
                                                                                                          cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTgg                             <  2:247226/139‑1 (MQ=255)
                                                                                                             aTGCGTACCGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa                          <  2:117706/139‑1 (MQ=255)
                                                                                                                   aCCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                     <  1:749472/139‑1 (MQ=255)
                                                                                                                    ccGATGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                   <  1:178403/139‑1 (MQ=255)
                                                                                                                       aTGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGaa                                                                                                                     >  2:844629/1‑38 (MQ=255)
                                                                                                                       aTGGCCGATCTGGCAGGGGAAAACTGCTGATGCTGGaa                                                                                                                     <  1:844629/38‑1 (MQ=38)
                                                                                                                         ggCCGATCTGGCAGGGGAAAACTGCTGATGCTGGaa                                                                                                                     >  1:1215510/1‑36 (MQ=255)
                                                                                                                         ggCCGATCTGGCAGGGGAAAACTGCTGATGCTGGaa                                                                                                                     <  2:1215510/36‑1 (MQ=37)
                                                                                                                          gCCGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAt             >  1:481997/1‑139 (MQ=255)
                                                                                                                            cGATCTGGCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg           <  2:254721/139‑1 (MQ=255)
                                                                                                                                  ggCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGATGCCgggg                                                                 >  2:799365/1‑79 (MQ=255)
                                                                                                                                  ggCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                 <  1:799365/79‑1 (MQ=255)
                                                                                                                                  ggCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg     <  2:88118/139‑1 (MQ=255)
                                                                                                                                   gCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggtggc    <  1:1203100/139‑1 (MQ=255)
                                                                                                                                   gCAGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcggc    >  2:38215/1‑139 (MQ=255)
                                                                                                                                     caggggAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa                                      >  1:717104/3‑104 (MQ=255)
                                                                                                                                     caggggAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAACATACACACTTCCGCGCGa                                      <  2:717104/102‑1 (MQ=255)
                                                                                                                                     aGGGGAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg  <  1:588327/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
GCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAG  >  NC_000913/4158917‑4159189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: