Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,759,680 (C)5→6 coding (844/1221 nt) sufS ← cysteine desulfurase, stimulated by SufE, selenocysteine lyase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,759,6751.C100.0% 100.7 / NA 30G283G (GGC→GGGsufScysteine desulfurase, stimulated by SufE, selenocysteine lyase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (14/16);  total (14/16)

TAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCT  >  NC_000913/1759547‑1759807
                                                                                                                                 |                                                                                                                                    
tAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGcc                                                                                                                                    >  2:76809/1‑131 (MQ=255)
tAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGcc                                                                                                                                    <  1:76809/131‑1 (MQ=255)
  gagagTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTAtt                                                                                                                           <  2:133695/139‑1 (MQ=255)
          gaTCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTAc                                                                                                                   >  2:157346/1‑139 (MQ=255)
          gaTCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTAc                                                                                                                   <  1:859869/139‑1 (MQ=255)
                 tACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTc                                                                                                            >  2:58879/1‑139 (MQ=255)
                     gATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAAc                                                                                                        <  1:157346/139‑1 (MQ=255)
                                 gATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                            <  1:294146/139‑1 (MQ=255)
                                 gATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                            <  1:444314/139‑1 (MQ=255)
                                 gATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCt                                                                                            <  2:386872/139‑1 (MQ=255)
                                      cgcATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGt                                                                                       <  1:504329/139‑1 (MQ=255)
                                        cATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGccccccggta                                                                                                                            <  2:1194349/99‑6 (MQ=255)
                                        cATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGccccccggta                                                                                                                            >  1:1194349/1‑94 (MQ=255)
                                                         tGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCg                                                                                                      <  2:988850/105‑1 (MQ=255)
                                                         tGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCg                                                                                                      >  1:988850/1‑105 (MQ=255)
                                                                     gCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCtt                                                                                           >  1:503487/1‑104 (MQ=255)
                                                                     gCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCtt                                                                                           <  2:503487/104‑1 (MQ=255)
                                                                       tATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                                      <  2:438197/139‑1 (MQ=255)
                                                                          gTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGCAGTGCCTTCACTCAGGCTGACGGTGGCGAt                                                   >  2:458319/1‑139 (MQ=255)
                                                                              ttaAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATa                                               >  1:798312/1‑139 (MQ=255)
                                                                                   ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCGATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACt                                                                     <  1:1174949/112‑1 (MQ=255)
                                                                                   ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCGATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACt                                                                     >  2:1174949/1‑112 (MQ=255)
                                                                                   ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAAcc                                          <  1:981860/139‑1 (MQ=255)
                                                                                   ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAAcc                                          >  2:981860/1‑139 (MQ=255)
                                                                                   ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGccccccggta                                                                                                                            >  2:1027084/1‑51 (MQ=255)
                                                                                   ccccAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGccccccggta                                                                                                                            <  1:1027084/56‑6 (MQ=255)
                                                                                        gcgcCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGcccc                                     >  2:31699/1‑139 (MQ=255)
                                                                                                      tCCAGCGCCGCGCCAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATAGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACggcggc                       <  1:458319/139‑1 (MQ=255)
                                                                                                           cgccgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCAtctc                  >  1:606396/1‑139 (MQ=255)
                                                                                                              cgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCg                                                     <  1:490025/101‑1 (MQ=255)
                                                                                                              cgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCg                                                     >  2:490025/1‑101 (MQ=255)
                                                                                                              cgcgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTg               >  1:40571/1‑139 (MQ=255)
                                                                                                                cgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                                      >  1:884529/1‑98 (MQ=255)
                                                                                                                cgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGc                                                      <  2:884529/98‑1 (MQ=255)
                                                                                                                cgccAAGACCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGca             >  2:57409/1‑139 (MQ=255)
                                                                                                                       aCCAATGATGCCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGc      <  1:58879/139‑1 (MQ=255)
                                                                                                                           atgatgcCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGcttct  <  1:31699/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
TAGAGAGTGAGATCCGGTACAGATTCCAGCTGTGATAGCGCATAATGCATCAGATTCTGTTCATACTCGGCTATGTTATTAAGCCCCAGCGCCGAAACATACTCCAGCGCCGCGCCAAGACCAATGATGCCCCCGGTATTGGGTGTACCGGCTTCAAACCGCCATGGTGCTTTGGTCCAGGTAGTGCCTTCACTCAGGCTGACGGTGGCGATCATAGAACCGCCCCCTTCCCACGGCGGCATCTCCTGCAACAAGGCTTCT  >  NC_000913/1759547‑1759807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: