Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,920,205 (T)7→8 intergenic (+62/‑18) yebT → / → rsmF MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,920,1981.T100.0% 67.2 / NA 22intergenic (+55/‑25)yebT/rsmFMCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (12/10);  total (12/10)

AGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGC  >  NC_000913/1920071‑1920317
                                                                                                                                |                                                                                                                       
aGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGttttttttgcc                                                                                                              <  1:551425/139‑5 (MQ=255)
  gCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                             <  1:1014346/139‑1 (MQ=255)
                  gCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATATTGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                             <  1:804433/123‑1 (MQ=255)
                  gCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                             >  2:804433/1‑123 (MQ=255)
                  gCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTgg                                                                                             >  2:292507/1‑139 (MQ=255)
                           tGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg                                                                                              <  1:1088622/129‑1 (MQ=255)
                           tGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg                                                                                              >  2:1088622/1‑129 (MQ=255)
                             aaCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCaa                                                                                        <  1:246817/133‑1 (MQ=255)
                             aaCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCaa                                                                                        >  2:246817/1‑133 (MQ=255)
                              aCCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCg                                                                                 >  1:147460/1‑139 (MQ=255)
                                                             cTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg                                                                                                <  1:1015586/93‑1 (MQ=255)
                                                             cTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCg                                                                                                >  2:1015586/1‑93 (MQ=255)
                                                                          gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                             >  1:571260/1‑67 (MQ=255)
                                                                          gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg                                                                                                             <  2:571260/67‑1 (MQ=255)
                                                                          gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATg                                     >  2:271104/1‑139 (MQ=255)
                                                                          gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGtttttttt                                                                                                                 >  1:829243/1‑61 (MQ=255)
                                                                          gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGtttttttt                                                                                                                 <  2:829243/62‑2 (MQ=255)
                                                                                            ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAttt                   >  1:341391/1‑139 (MQ=255)
                                                                                                 gcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                    >  2:1018440/1‑69 (MQ=255)
                                                                                                 gcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca                                                                                    <  1:1018440/69‑1 (MQ=255)
                                                                                                     gAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg                                                                                              <  1:722645/55‑1 (MQ=255)
                                                                                                     gAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg                                                                                              >  2:722645/1‑55 (MQ=255)
                                                                                                             aTGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTgccgc  <  1:271104/139‑1 (MQ=255)
                                                                                                                        cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACaa                                                    <  1:459051/78‑1 (MQ=255)
                                                                                                                        cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACaa                                                    >  2:459051/1‑78 (MQ=255)
                                                                                                                                |                                                                                                                       
AGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGC  >  NC_000913/1920071‑1920317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: