| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,240,754 | (C)5→6 | coding (915/1041 nt) | galS ← | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,240,749 | 1 | . | C | 97.9% | 166.3 / ‑3.3 | 48 | coding (920/1041 nt) | galS | galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (23/24); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAG > NC_000913/2240614‑2240868 | caGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc < 1:188044/139‑1 (MQ=255) tGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccctg > 2:1223052/1‑136 (MQ=255) gAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑ccc‑ccctgc > 1:1174516/1‑135 (MQ=255) gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCa > 1:774023/1‑139 (MQ=255) gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCa > 1:457531/1‑139 (MQ=255) aTCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg < 2:457531/139‑1 (MQ=255) ccTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTgg > 2:548669/1‑139 (MQ=255) cTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGc < 1:1223052/139‑1 (MQ=255) aTTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTaa > 1:868631/1‑139 (MQ=255) ttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCg > 1:609234/1‑139 (MQ=255) tAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTg < 2:1174516/139‑1 (MQ=255) aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTGATATCGCCATTGa < 2:868631/139‑1 (MQ=255) aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 1:1272384/139‑1 (MQ=255) gTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGaa > 2:713457/1‑139 (MQ=255) gATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg > 2:1200179/1‑116 (MQ=255) gATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCg < 1:1200179/116‑1 (MQ=255) ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAAttt < 2:1095984/122‑1 (MQ=255) ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAAttt > 1:1095984/1‑122 (MQ=255) ccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGAt > 2:392057/1‑139 (MQ=255) cATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa > 2:89362/1‑125 (MQ=255) cATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa < 1:89362/125‑1 (MQ=255) tCTGGGGTGTTGCGACAGAATGACGACTCTCTAACGTCGGCATGCAACAATAGCTGGGACGAGGTTCAATATTGCGTGGTG‑CCCTCCTTCAAGGCCACTTCTGTGGCTGATTTCTCCATTGAAGCAATGGGATAACGCa < 2:585101/139‑1 (MQ=255) tCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 1:1258767/139‑1 (MQ=255) cGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTc > 2:245705/1‑139 (MQ=255) gtgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCg > 2:257795/1‑139 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGcc < 2:991688/87‑1 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGcc > 1:991688/1‑87 (MQ=255) ttGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTa > 2:1153855/1‑139 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTc > 1:593475/1‑91 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTc < 2:593475/91‑1 (MQ=255) tGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTaa > 2:1163918/1‑139 (MQ=255) gACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTg < 1:1163918/139‑1 (MQ=255) aCTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACgg < 1:1153855/139‑1 (MQ=255) aCGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGTCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGa < 2:774023/139‑1 (MQ=255) gTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc < 1:711520/109‑1 (MQ=255) gTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTc > 2:711520/1‑109 (MQ=255) gctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 1:1116436/1‑87 (MQ=255) gctggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:1116436/87‑1 (MQ=255) tggcACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga < 1:257795/139‑1 (MQ=255) cACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCg > 2:62529/1‑114 (MQ=255) cACGAGGATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCg < 1:62529/114‑1 (MQ=255) ggATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag < 2:609234/139‑1 (MQ=255) ggATCAATATTGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgag < 1:713457/139‑1 (MQ=255) tGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa < 2:612545/67‑1 (MQ=255) tGCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa > 1:612545/1‑67 (MQ=255) gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa > 1:46678/1‑54 (MQ=255) gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa < 2:46678/54‑1 (MQ=255) gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAat > 2:594172/1‑106 (MQ=255) gCCTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAat < 1:594172/106‑1 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc < 1:2826/96‑1 (MQ=255) ccTGCTGCCCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc > 2:2826/1‑96 (MQ=255) | CAGATTGAAAGCGGTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCC‑CCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAG > NC_000913/2240614‑2240868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |