| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,511,746 | (A)8→9 | coding (599/906 nt) | yhfZ ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,511,738 | 1 | . | A | 98.2% | 169.0 / ‑3.5 | 54 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (28/25); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01 | |||||||||||
CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511604‑3511869 | cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑aaaa > 2:95584/1‑139 (MQ=255) tCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑aaaaa‑a > 1:327722/1‑139 (MQ=255) tctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑aaaaa‑aaaaca < 1:96613/139‑4 (MQ=255) cccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTc < 2:327722/139‑1 (MQ=255) cccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTc < 2:1072281/139‑1 (MQ=255) cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGAt > 2:34471/1‑139 (MQ=255) cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGAt > 1:844903/1‑139 (MQ=255) cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGAt > 1:671723/1‑139 (MQ=255) ggTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGAtttt > 2:15721/1‑139 (MQ=255) gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGAt > 1:1099811/1‑139 (MQ=255) gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGAt < 2:197062/139‑1 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATccgccg < 1:95584/139‑1 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATccgccg < 1:826269/139‑1 (MQ=255) tCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGaa < 2:671723/139‑1 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:1192648/139‑1 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGAAATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:1024540/139‑1 (MQ=255) ccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt > 2:613918/1‑139 (MQ=255) ttCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGcc > 2:116764/1‑139 (MQ=255) gATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAc < 1:650966/139‑1 (MQ=255) aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:1232572/139‑1 (MQ=255) ccGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCt > 1:741333/1‑139 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTg > 2:843831/1‑106 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTg < 1:843831/106‑1 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:691546/1‑139 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:1136749/1‑139 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACAGCCA‑‑AAAAA‑AAACCTCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACCCGCtt > 2:1041701/1‑139 (MQ=255) cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGt > 2:505748/1‑139 (MQ=255) gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:973501/139‑1 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATg > 2:966603/1‑75 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATg < 1:966603/75‑1 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt > 2:45972/1‑130 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 1:45972/130‑1 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc > 2:404793/1‑139 (MQ=255) gTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc < 1:1004151/139‑1 (MQ=255) cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt < 2:1136749/139‑1 (MQ=255) aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:1052670/139‑1 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGTACGGTTAt < 1:842234/76‑1 (MQ=255) agagaTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt > 2:842234/1‑76 (MQ=255) gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAA‑AAACATCGGCCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACCGCAAACCAACCGGTGCTCGCCAAc > 1:201161/1‑139 (MQ=255) gaTCGACTCGTTCCACATCACTACAGCG‑‑‑AAAAAGAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGAGTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGt < 2:1074271/139‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATccgccg > 1:803477/1‑63 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATccgccg < 2:803477/63‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:943254/83‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:81868/1‑83 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:81868/83‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:943254/1‑83 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 1:572826/1‑139 (MQ=255) aCTCGTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgtg > 2:90665/1‑139 (MQ=255) gTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg < 1:404793/139‑1 (MQ=255) gTTCCACATCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg > 2:543687/1‑139 (MQ=255) cacaTCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGccc < 1:613918/139‑1 (MQ=255) caTCACTACCGCCA‑‑AAAAA‑AAACATCGGTGATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTGACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc > 1:865275/1‑118 (MQ=255) caTCACTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:865275/118‑1 (MQ=255) cTACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc > 1:1112932/1‑139 (MQ=255) tACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 1:654686/1‑48 (MQ=255) tACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:654686/48‑1 (MQ=255) tACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:463145/1‑139 (MQ=255) tACCGCCA‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:46618/1‑139 (MQ=255) | CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511604‑3511869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |