Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,728,118	(C)8→9	coding (1254/1455 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,110	1	.	C	100.0%	162.1 / NA	49	D421G (GAT→GGT)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/28);  total (21/28)

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC  >  NC_000913/3727976‑3728234                                                                                                                                        |                                                                                                                                gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                            <  1:1048612/139‑1 (MQ=255) gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                            <  1:294480/139‑1 (MQ=255)         gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccccgta                                                                                                                    <  1:1047529/139‑5 (MQ=255)          cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccccgtac                                                                                                                   <  1:711391/139‑6 (MQ=255)                    cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAg                                                                                                          >  2:896385/1‑139 (MQ=255)                         cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTTCGCCAGCCTTGCTGCGCCCAGTGCTTGCCCCACATC‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                     >  2:1147401/1‑139 (MQ=255)                           ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                   <  1:896385/139‑1 (MQ=255)                            gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccc                                                                                                                        >  1:222861/1‑116 (MQ=255)                            gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccc                                                                                                                        <  2:222861/116‑1 (MQ=255)                               gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                               <  2:86524/139‑1 (MQ=255)                                 tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cc                                                                                                                              >  1:762476/1‑105 (MQ=255)                                 tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cc                                                                                                                              <  2:762476/105‑1 (MQ=255)                                   cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                            >  1:776211/1‑105 (MQ=255)                                   cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccc                                                                                                                            <  2:776211/105‑1 (MQ=255)                                     aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                         >  2:442806/1‑139 (MQ=255)                                      cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                                                        >  2:455762/1‑139 (MQ=255)                                      cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATc                                                                                        >  2:203258/1‑139 (MQ=255)                                         ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                     <  2:1281086/139‑1 (MQ=255)                                         ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                     >  1:465527/1‑139 (MQ=255)                                                 gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAt                                                                                                                                  >  2:469626/1‑87 (MQ=255)                                                 gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAt                                                                                                                                  <  1:469626/87‑1 (MQ=255)                                                   aGTTGCGGCAACAATTCAATGAGCGGTTTCTCTGGATTCGCCTCGATCTGCGCCAGCCTTGCTGCGACCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           <  1:290016/139‑1 (MQ=255)                                                   aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           >  1:390425/1‑139 (MQ=255)                                                   aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                           <  2:1169790/139‑1 (MQ=255)                                                      tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                        <  2:995344/139‑1 (MQ=255)                                                      tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                        <  1:69867/139‑1 (MQ=255)                                                       gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                       <  1:665673/139‑1 (MQ=255)                                                          gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTC‑CCCCCCCCCGACCGTAAGCGAGCTGCTTTCCCCCGCTATCCGCCAGCTTCCGACCCCacg                                                                    >  1:1250478/1‑137 (MQ=255)                                                            aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑Ac                                                                <  1:442806/139‑1 (MQ=255)                                                                         gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                  >  1:117428/1‑110 (MQ=255)                                                                         gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                  <  2:117428/110‑1 (MQ=255)                                                                         gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGcgccc                                                   >  2:425345/1‑139 (MQ=255)                                                                         gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGcgccc                                                   <  1:1108953/139‑1 (MQ=255)                                                                          cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                   >  1:1080310/1‑92 (MQ=255)                                                                          cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                   <  2:1080310/92‑1 (MQ=255)                                                                           gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGcgccccg                                                 >  1:763623/1‑139 (MQ=255)                                                                             tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGCACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGcgccccgcc                                               <  2:1256333/139‑1 (MQ=255)                                                                             tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCCTACTGTTATCGAGCTGCTGACCGCTGATAACCGCCACAATCTGACGCGTGG‑‑ACTCAATACGccccccgcc                                               >  2:278100/1‑139 (MQ=255)                                                                              ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGcgccccgccc                                              <  2:763623/139‑1 (MQ=255)                                                                                   tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAAt                                         <  2:908244/139‑1 (MQ=255)                                                                                   tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAAt                                         <  1:278100/139‑1 (MQ=255)                                                                                   tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAAt                                         <  1:425345/139‑1 (MQ=255)                                                                                   tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAAt                                         <  1:1129517/139‑1 (MQ=255)                                                                                   tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCCTACGGTCATCGAGCCGCTGACCGCTGATATCCGCCAGCACCTGACGCCAGA‑‑CCCCACTACGCCCCCCGCCCCCAAt                                         >  2:1129517/1‑139 (MQ=255)                                                                                    ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                         >  1:863908/1‑108 (MQ=255)                                                                                    ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCACAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                         <  2:863908/108‑1 (MQ=255)                                                                                          gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTa                                  <  1:203258/139‑1 (MQ=255)                                                                                          gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTa                                  >  1:1120180/1‑139 (MQ=255)                                                                                          gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccccgta                                                                                                                    <  1:851749/58‑5 (MQ=255)                                                                                          gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑cccccccccgta                                                                                                                    >  2:851749/1‑54 (MQ=255)                                                                                                  gtataagagacaggctgcGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                          <  1:179991/126‑1 (MQ=255)                                                                                                  cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                          <  1:455762/139‑1 (MQ=255)                                                                                                   tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                         <  2:465527/139‑1 (MQ=255)                                                                                                     cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                      >  1:1116314/1‑94 (MQ=255)                                                                                                     cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                      <  2:1116314/94‑1 (MQ=255)                                                                                                          gCCTTGCTGCGCCGAGTGCTGGCCCCTCATC‑CCCCCCCCGTACGGTAATCGGGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                  <  2:1250478/139‑1 (MQ=255)                                                                                                           ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTTACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑CCTCACTACGCGCCCCGCCCCCa                                           >  2:424910/1‑113 (MQ=255)                                                                                                           ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                 >  1:1108481/1‑139 (MQ=255)                                                                                                           ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCa                                           <  1:424910/113‑1 (MQ=255)                                                                                                           ccTTGCTGCGCCCAGTGCTGGCCCCACATC‑CCCCCCCCGCACCGTAATCGAGCTGCTGACCCCCGATATCCGCCAGCAACCGACCCCAGTCCCCTCACTACGCCCCCCCCCCCCAAACAACGTAACACTTTGCCGTTTa                   >  1:291821/1‑139 (MQ=255)                                                                                                                   cgcCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg          <  1:1297716/139‑1 (MQ=255)                                                                                                                          tGCTGGCCCCACATC‑CCCCCCCCGTCCGGTAAACGAGCTGCTGAACGCTGATATCCCCCAGCATCAGACACCAGT‑‑ACTCACTACGCGCCCCCCCCCCAATCAACGTCAAACTTTGCGGCGTAATAcctgtctcttctac  >  2:179991/1‑127 (MQ=255)                                                                                                                          tGCTGGCCCCACATC‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCTAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGTAGGCATGCAc  <  2:390425/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGT‑‑ACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC  >  NC_000913/3727976‑3728234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑