Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,897,050 +G coding (277/666 nt) yieH → phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,0461.G98.6% 255.6 / ‑3.6 73coding (273/666 nt)yieHphosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (38/34);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177
                                                                                                                                            |                                                                                                                                   
tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      >  2:422177/1‑139 (MQ=255)
tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      >  2:236307/1‑139 (MQ=255)
tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      <  2:559469/139‑1 (MQ=255)
   cAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAggg                                                                                                                                  <  2:1285018/139‑1 (MQ=255)
           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                           >  2:840170/1‑139 (MQ=255)
           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                           >  2:580073/1‑139 (MQ=255)
                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAGCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAg                                                                                                                                    >  2:1209218/1‑122 (MQ=255)
                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCt                                                                                                                    >  1:692202/1‑139 (MQ=255)
                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAgggggctg                                                                                                                             <  1:939913/129‑5 (MQ=255)
                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAgggggctg                                                                                                                             >  2:939913/1‑125 (MQ=255)
                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAg                                                                                                                                    <  1:1209218/122‑1 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTcc                                                                                                                     >  2:262968/1‑137 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTcc                                                                                                                     <  1:262968/137‑1 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:859993/1‑139 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:880884/1‑139 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:1177218/1‑139 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:1204427/1‑139 (MQ=255)
                        aaTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              >  2:1081201/1‑139 (MQ=255)
                               gATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACt                                                                                                       <  2:1260837/139‑1 (MQ=255)
                                  atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGTAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  1:126829/139‑1 (MQ=255)
                                  atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  2:126829/1‑139 (MQ=255)
                                  atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  2:1204427/139‑1 (MQ=255)
                                    tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  2:1245185/1‑139 (MQ=255)
                                     gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  1:236307/139‑1 (MQ=255)
                                     gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  2:880884/139‑1 (MQ=255)
                                     gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  1:498773/139‑1 (MQ=255)
                                         cccTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt                                                                                             <  1:1081201/139‑1 (MQ=255)
                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCt                                                                                                                       >  2:131392/1‑111 (MQ=255)
                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCt                                                                                                                       <  1:131392/111‑1 (MQ=255)
                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgt                                                                                           >  1:1169914/1‑139 (MQ=255)
                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              <  2:509515/136‑1 (MQ=255)
                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              >  1:509515/1‑136 (MQ=255)
                                             tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGATCGATTCAGAACTGGACGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgggt                                                                                         >  2:1273445/1‑139 (MQ=255)
                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCt                                                                                                                       >  2:970240/1‑102 (MQ=255)
                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCt                                                                                                                       <  1:970240/102‑1 (MQ=255)
                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  2:408842/1‑139 (MQ=255)
                                                      tgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTaa                                                                                <  1:1273445/139‑1 (MQ=255)
                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  1:934039/139‑1 (MQ=255)
                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  1:580073/139‑1 (MQ=255)
                                                           cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCaa                                                                                                <  2:929959/118‑1 (MQ=255)
                                                           cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCaa                                                                                                >  1:929959/1‑118 (MQ=255)
                                                           cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGcc                                                                           >  1:271779/1‑139 (MQ=255)
                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  1:811561/1‑110 (MQ=255)
                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  2:811561/110‑1 (MQ=255)
                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa                                                                       >  1:1051233/1‑139 (MQ=255)
                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa                                                                       >  2:1157703/1‑139 (MQ=255)
                                                                gCGAAAACAGAAGCTGGACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAgggggctg                                                                                                                             >  2:9669/1‑79 (MQ=255)
                                                                gCGAAAACAGAAGCTGGACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAgggggctg                                                                                                                             <  1:9669/83‑5 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  2:16230/104‑1 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  1:16230/1‑104 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  2:706753/109‑1 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  1:706753/1‑109 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  2:880045/114‑1 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                      >  1:560471/1‑139 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAgggggctg                                                                                                                             <  2:955270/83‑5 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAgggggctg                                                                                                                             >  1:955270/1‑79 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACAAGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               >  1:880045/1‑114 (MQ=255)
                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  1:984034/99‑1 (MQ=255)
                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  2:984034/1‑99 (MQ=255)
                                                                              tGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca                                                        <  1:840170/139‑1 (MQ=255)
                                                                                       ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               <  2:271779/139‑1 (MQ=255)
                                                                                       ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               >  2:514078/1‑139 (MQ=255)
                                                                                        tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              <  2:222870/139‑1 (MQ=255)
                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                       >  2:843581/1‑139 (MQ=255)
                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCg                                                                                                             >  2:659742/1‑64 (MQ=255)
                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCg                                                                                                             <  1:659742/64‑1 (MQ=255)
                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGt                                 >  2:1036921/1‑137 (MQ=255)
                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGt                                 <  1:1036921/137‑1 (MQ=255)
                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTg                               <  2:692202/139‑1 (MQ=255)
                                                                                                        cTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGc                              <  1:1114391/139‑1 (MQ=255)
                                                                                                              tGTTCGATTCTGAACTGGAAGCAATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  2:631277/1‑58 (MQ=37)
                                                                                                              tGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  1:631277/58‑1 (MQ=255)
                                                                                                                     ttCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACa                                                                  >  2:20987/1‑90 (MQ=255)
                                                                                                                     ttCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACa                                                                  <  1:20987/90‑1 (MQ=255)
                                                                                                                          aaCTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               >  2:207928/1‑56 (MQ=255)
                                                                                                                          aaCTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  1:207928/56‑1 (MQ=255)
                                                                                                                               gAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc                                                     <  1:1038634/93‑1 (MQ=255)
                                                                                                                               gAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc                                                     >  2:1038634/1‑93 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  1:141181/1‑36 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  2:141181/36‑1 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                 <  1:33200/60‑1 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                 >  2:33200/1‑60 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:843581/139‑1 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:422177/139‑1 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  >  1:965707/1‑139 (MQ=255)
                                                                                                                                            |                                                                                                                                   
TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: