Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,857,1450TA47.1% 4.3 / 52.7 34L18H (CTC→CAC) ‡mutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (8/10);  new base A (12/4);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 9.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTC  >  NC_000913/2857034‑2857253
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tACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGAt                                                                                   >  2:841340/1‑139 (MQ=255)
                          cccATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGAtt                                                         >  1:567010/1‑139 (MQ=255)
                                   tatCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc                                                <  2:1281071/139‑1 (MQ=255)
                                    atCAGGGAAACGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACt                                               >  1:870199/1‑139 (MQ=255)
                                        gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 <  1:418324/133‑1 (MQ=255)
                                        gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 >  2:418324/1‑133 (MQ=255)
                                        gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGttt                                           >  2:496998/1‑139 (MQ=255)
                                        gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGttt                                           >  1:367532/1‑139 (MQ=255)
                                             ccGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 <  2:671368/128‑1 (MQ=255)
                                             ccGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 >  1:671368/1‑128 (MQ=255)
                                              cGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATgac                                     <  2:870199/139‑1 (MQ=255)
                                              cGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATgac                                     >  2:1188336/1‑139 (MQ=255)
                                                     aaCCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCctg                                                                               <  2:601531/90‑1 (MQ=255)
                                                     aaCCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCctg                                                                               >  1:601531/1‑90 (MQ=255)
                                                          cATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCctg                                                                               <  1:353933/85‑1 (MQ=255)
                                                          cATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCctg                                                                               >  2:353933/1‑85 (MQ=255)
                                                           aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 <  1:1285335/114‑1 (MQ=255)
                                                           aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 >  2:1285335/1‑114 (MQ=255)
                                                            tGAGTGCAATATAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCGAAAcgcgc                       <  1:841340/139‑1 (MQ=255)
                                                               gTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGa                                                                  >  2:767916/1‑93 (MQ=255)
                                                               gTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGa                                                                  <  1:767916/93‑1 (MQ=255)
                                                                tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 <  1:877373/109‑1 (MQ=255)
                                                                tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa                                                 >  2:877373/1‑109 (MQ=255)
                                                                tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCg                   >  2:711151/1‑139 (MQ=255)
                                                                 gCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGc                  <  2:567010/139‑1 (MQ=255)
                                                                  cAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATg                                                   >  1:458157/1‑105 (MQ=255)
                                                                  cAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGa                                                  <  2:1170579/106‑1 (MQ=255)
                                                                  cAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGa                                                  >  1:1170579/1‑106 (MQ=255)
                                                                  cAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCa                 >  1:888275/1‑139 (MQ=255)
                                                                  cAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATg                                                   <  2:458157/105‑1 (MQ=255)
                                                                  cAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCa                 >  2:1168898/1‑139 (MQ=255)
                                                                        aaaaTTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActgct           >  1:242329/1‑139 (MQ=255)
                                                                        aaaaTTTCGACGCCCATACGCCCATGATGCAGCAGTGATAGTCGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActgct           <  1:1188336/139‑1 (MQ=255)
                                                                                 acgcccatacgcccatGATGCAGCAGTGATAGTC‑‑GCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTc  >  2:1271618/1‑139 (MQ=255)
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TACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTATCTCAGGCTGAAAGCCCAGCATCCCGAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTC  >  NC_000913/2857034‑2857253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: