Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	49,765	C→T	intergenic (+134/‑58)	kefC → / → folA	potassium:proton antiporter/dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	49,765	0	C	T	100.0%	187.7 / NA	58	intergenic (+134/‑58)	kefC/folA	potassium:proton antiporter/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

AATAAAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCC  >  NC_000913/49630‑49896                                                                                                                                        |                                                                                                                                    aataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgac                                                                                                                                  >  2:269549/1‑139 (MQ=255)  ataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacg                                                                                                                                 <  1:213177/139‑1 (MQ=255)        agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                                                                                           >  1:1144656/1‑139 (MQ=255)        agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                                                                                           <  2:1144656/139‑1 (MQ=255)        agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                                                                                           >  2:1049668/1‑139 (MQ=255)        agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                                                                                           >  1:736314/1‑139 (MQ=255)         gTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                                                                          <  1:263262/139‑1 (MQ=255)            aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACg                                                                                                                       >  2:395511/1‑139 (MQ=255)              gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     <  2:987308/139‑1 (MQ=255)              gTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     <  2:1201169/139‑1 (MQ=255)                  aTCACACTCTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      >  2:513269/1‑134 (MQ=255)                  aTCACACTCTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      <  1:513269/134‑1 (MQ=255)                      cacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGtat                                                                                                             >  2:377280/1‑139 (MQ=255)                         tttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGt                                                                                                          >  1:193272/1‑139 (MQ=255)                                   aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      <  2:322252/117‑1 (MQ=255)                                   aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      >  1:322252/1‑117 (MQ=255)                                     tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCttt                                                                                                                   >  2:166902/1‑118 (MQ=255)                                     tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCttt                                                                                                                   <  1:166902/118‑1 (MQ=255)                                                              cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa                                                                     >  2:890074/1‑139 (MQ=255)                                                                gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                                                                          >  1:927491/1‑84 (MQ=255)                                                                gcgAGTCAAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                                                                          <  2:927491/84‑1 (MQ=255)                                                                 cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATCTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAgcc                                                                  >  1:711533/1‑137 (MQ=255)                                                                             gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  1:395511/139‑1 (MQ=255)                                                                             gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  1:377280/139‑1 (MQ=255)                                                                             gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  1:1049668/139‑1 (MQ=255)                                                                             gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  1:269549/139‑1 (MQ=255)                                                                             gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  1:890074/139‑1 (MQ=255)                                                                             gagagCGTGGACTCGCCAGCAGAATATAAAATCTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  2:711533/139‑1 (MQ=255)                                                                              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTAc                                                                                                                        <  2:806657/72‑1 (MQ=255)                                                                              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTAc                                                                                                                        <  2:248962/72‑1 (MQ=255)                                                                              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTAc                                                                                                                        >  1:248962/1‑72 (MQ=255)                                                                              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTAc                                                                                                                        >  1:806657/1‑72 (MQ=255)                                                                              agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGtt                                                     >  1:1132540/1‑139 (MQ=255)                                                                               gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCttt                                                                                                                   <  2:685241/76‑1 (MQ=255)                                                                               gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCttt                                                                                                                   >  1:685241/1‑76 (MQ=255)                                                                                  cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCg                                                 >  1:520958/1‑139 (MQ=255)                                                                                     ggACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTa                                              >  2:591206/1‑139 (MQ=255)                                                                                      gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacg                                                                                                                                 <  2:1108306/55‑1 (MQ=255)                                                                                      gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTtgacg                                                                                                                                 >  1:1108306/1‑55 (MQ=255)                                                                                      gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      >  2:1096059/1‑130 (MQ=255)                                                                                      gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGt                                                      <  1:1096059/130‑1 (MQ=255)                                                                                                cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                   <  2:736314/139‑1 (MQ=255)                                                                                                    atatAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGc                               <  2:1236961/139‑1 (MQ=255)                                                                                                    atatAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGc                               <  2:615302/139‑1 (MQ=255)                                                                                                     tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                                                                          <  2:612080/47‑1 (MQ=255)                                                                                                     tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                                                                          >  1:612080/1‑47 (MQ=255)                                                                                                     tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTa                                                                                                                         <  1:435805/48‑1 (MQ=255)                                                                                                     tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTa                                                                                                                         >  2:435805/1‑48 (MQ=255)                                                                                                     tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTgcg                                                          >  2:830697/1‑111 (MQ=255)                                                                                                     tataAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTgcg                                                          <  1:830697/111‑1 (MQ=255)                                                                                                          aaTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     >  1:966412/1‑47 (MQ=255)                                                                                                          aaTTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCt                                                                                                                     <  2:966412/47‑1 (MQ=255)                                                                                                             tttCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                                                                                           <  2:635900/38‑1 (MQ=255)                                                                                                             tttCCTCAACATCATCCTCGCACCAGTTGACGACGGtt                                                                                                                           >  1:635900/1‑38 (MQ=255)                                                                                                               tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      >  1:1226526/1‑41 (MQ=255)                                                                                                               tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGc                                                                                                                      <  2:1226526/41‑1 (MQ=255)                                                                                                               tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGc                    >  2:105174/1‑139 (MQ=255)                                                                                                               tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTCACGc                                                                                                                      <  2:1146302/41‑1 (MQ=255)                                                                                                               tCCTCAACATCATCCTCGCACCAGTTGACGACGGTTCACGc                                                                                                                      >  1:1146302/1‑41 (MQ=255)                                                                                                                 cTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                                                                          <  2:325070/35‑1 (MQ=255)                                                                                                                 cTCAACATCATCCTCGCACCAGTTGACGACGGttt                                                                                                                          >  1:325070/1‑35 (MQ=255)                                                                                                                                 cACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcc  >  1:1196449/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                    AATAAAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCC  >  NC_000913/49630‑49896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑