Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	477,025	G→A	*118* (TAG→TAA)	ybaA →	DUF1428 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	477,025	0	G	A	100.0%	177.8 / NA	55	*118* (TAG→TAA)	ybaA	DUF1428 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

AAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATG  >  NC_000913/476896‑477153                                                                                                                                  |                                                                                                                                 aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGa                                                                                                                         >  2:872419/1‑139 (MQ=255)  aaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGAc                                                                                                                        <  2:734465/139‑1 (MQ=255)   aaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACg                                                                                                                       >  2:767464/1‑139 (MQ=255)      agGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGc                                                                                                                    >  1:125586/1‑139 (MQ=255)            gcgACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgcg                                                                                                              >  2:538867/1‑139 (MQ=255)                     ctAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGt                                                                                                     <  2:125586/139‑1 (MQ=255)                          cAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGcc                                                                                                >  1:33532/1‑139 (MQ=255)                               gatgatGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaa                                                                                           >  1:429944/1‑139 (MQ=255)                                atgatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaaa                                                                                          <  2:137113/139‑1 (MQ=255)                                atgatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaaa                                                                                          <  1:538867/139‑1 (MQ=255)                                   atgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTc                                                                                       >  2:627576/1‑139 (MQ=255)                                         gACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAAt                                                                                 >  2:950400/1‑139 (MQ=255)                                         gACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAAt                                                                                 >  1:614635/1‑139 (MQ=255)                                               cGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                           <  2:472914/139‑1 (MQ=255)                                               cGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                           <  1:950400/139‑1 (MQ=255)                                                  aTGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCgg                                                                        <  2:614635/139‑1 (MQ=255)                                                   tGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGc                                                                       <  2:33532/139‑1 (MQ=255)                                                     aaaGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGccc                                                                     <  2:429944/139‑1 (MQ=255)                                                            tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTaa                                                              >  1:299547/1‑139 (MQ=255)                                                                     ccATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAtcttc                                                     >  2:502810/1‑139 (MQ=255)                                                                        tGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCttt                                                  <  1:502810/139‑1 (MQ=255)                                                                            gTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCGGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGt                                              >  2:752210/1‑139 (MQ=255)                                                                                gACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGTCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATg                                          <  1:872419/139‑1 (MQ=255)                                                                                                         gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                           >  1:535841/1‑81 (MQ=255)                                                                                                         gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                           <  2:321227/81‑1 (MQ=255)                                                                                                         gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                           <  2:535841/81‑1 (MQ=255)                                                                                                         gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                           >  1:321227/1‑81 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGc                                                                       <  1:451633/84‑1 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGc                                                                       >  2:451633/1‑84 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCtt                                      >  1:244830/1‑117 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCtt                                      >  2:177474/1‑117 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCtt                                      <  2:244830/117‑1 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCtt                                      <  1:177474/117‑1 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                       <  1:40073/116‑1 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                       >  2:40073/1‑116 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagcca                          <  2:640747/129‑1 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagcca                          >  1:640747/1‑129 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                >  2:492334/1‑139 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                >  1:1294143/1‑139 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                <  2:299547/139‑1 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                >  1:401116/1‑139 (MQ=255)                                                                                                          aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                >  1:524989/1‑139 (MQ=255)                                                                                                            tCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCtgtg                                                                                                                >  1:1072794/1‑41 (MQ=255)                                                                                                            tCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCtgtg                                                                                                                <  2:1072794/41‑1 (MQ=255)                                                                                                            tCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTa              >  2:1040551/1‑139 (MQ=255)                                                                                                              gAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGt            >  1:948961/1‑139 (MQ=255)                                                                                                               aGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTg           <  2:524989/139‑1 (MQ=255)                                                                                                               aGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTg           <  1:930415/139‑1 (MQ=255)                                                                                                                gTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                        <  2:232985/109‑1 (MQ=255)                                                                                                                gTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                        >  1:232985/1‑109 (MQ=255)                                                                                                                gTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTg                                  >  2:357636/1‑115 (MQ=255)                                                                                                                gTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTg                                  <  1:357636/115‑1 (MQ=255)                                                                                                                        tcGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATg  <  2:263981/139‑1 (MQ=255)                                                                                                                           acgaATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTg                                  >  2:1115530/1‑104 (MQ=255)                                                                                                                           acgaATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTg                                  <  1:1115530/104‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 AAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATG  >  NC_000913/476896‑477153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑