Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	675,570	C→T	*326* (TAG→TAA)	ybeQ ←	Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	675,570	0	C	T	100.0%	158.6 / NA	49	*326* (TAG→TAA)	ybeQ	Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

GAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGG  >  NC_000913/675435‑675705                                                                                                                                        |                                                                                                                                        gAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTAc                                                                                                                                      <  1:820221/139‑1 (MQ=255) gAAATGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTAc                                                                                                                                      >  2:1054050/1‑139 (MQ=255)       cTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTaaa                                                                                                                                >  1:634688/1‑139 (MQ=255)               aTGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGt                                                                                                                        <  2:634688/139‑1 (MQ=255)                  cccGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGt                                                                                                                                   <  2:97769/125‑1 (MQ=255)                  cccGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGt                                                                                                                                   >  1:97769/1‑125 (MQ=255)                                     cAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAt                                                                                                 >  1:110625/1‑139 (MQ=255)                                                   cccGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGAt                                                                                   >  2:993647/1‑139 (MQ=255)                                                       tttGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACt                                                                               <  1:668801/139‑1 (MQ=255)                                                        ttGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTg                                                                              <  1:154161/139‑1 (MQ=255)                                                              cGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCa                                                                                                                          >  2:1180834/1‑90 (MQ=255)                                                              cGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCa                                                                                                                          <  1:1180834/90‑1 (MQ=255)                                                                  aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                    >  1:283187/1‑139 (MQ=255)                                                                            ttCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCga                                                          <  2:110625/139‑1 (MQ=255)                                                                                cacaGCAATAAATAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                      <  2:258226/139‑1 (MQ=255)                                                                                    gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtct                                                                                               >  2:921022/1‑94 (MQ=255)                                                                                    gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtct                                                                                               <  1:921022/94‑1 (MQ=255)                                                                                     cAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTc                                                 >  1:712659/1‑139 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   >  2:813499/1‑79 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   <  1:813499/79‑1 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGt                                                                                          >  1:729986/1‑88 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGt                                                                                          <  2:729986/88‑1 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGACTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            <  1:558724/102‑1 (MQ=255)                                                                                               aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGACTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            >  2:558724/1‑102 (MQ=255)                                                                                                    gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTc                                  >  2:199998/1‑139 (MQ=255)                                                                                                    gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTc                                  >  2:173870/1‑139 (MQ=255)                                                                                                    gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTc                                  >  1:528146/1‑139 (MQ=255)                                                                                                    gggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTc                                  >  1:729178/1‑139 (MQ=255)                                                                                                       ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            >  2:694164/1‑94 (MQ=255)                                                                                                       ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGaa                                                                            <  1:694164/94‑1 (MQ=255)                                                                                                        cGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcc                              <  2:283187/139‑1 (MQ=255)                                                                                                         gAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcct                             >  2:683748/1‑139 (MQ=255)                                                                                                         gAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcct                             >  1:1036700/1‑139 (MQ=255)                                                                                                          aaTGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   >  2:767044/1‑68 (MQ=255)                                                                                                          aaTGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   <  1:767044/68‑1 (MQ=255)                                                                                                             gAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTtctc                         <  1:199998/139‑1 (MQ=255)                                                                                                               aaTTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                       <  2:1093124/139‑1 (MQ=255)                                                                                                                    cccGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTg                  >  1:695067/1‑139 (MQ=255)                                                                                                                      cGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                      >  1:983881/1‑117 (MQ=255)                                                                                                                      cGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                      <  2:983881/117‑1 (MQ=255)                                                                                                                       ggCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtct                                                                                               >  2:1271758/1‑59 (MQ=255)                                                                                                                       ggCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAtct                                                                                               <  1:1271758/59‑1 (MQ=255)                                                                                                                         cccTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   <  2:100626/53‑1 (MQ=255)                                                                                                                         cccTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCc‑gactgac                                                                                                   >  1:100626/1‑53 (MQ=255)                                                                                                                         cccTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACa             <  1:173870/139‑1 (MQ=255)                                                                                                                                ttttATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACAACTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc      <  1:772592/139‑1 (MQ=255)                                                                                                                                   tATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAg   >  2:598446/1‑139 (MQ=255)                                                                                                                                    aTCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg  >  2:584803/1‑139 (MQ=255)                                                                                                                                    aTCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg  <  1:683748/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGG  >  NC_000913/675435‑675705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑