Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	821,506	G→A	*238* (TAG→TAA)	ybhM →	BAX Inhibitor‑1 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	821,506	0	G	A	100.0%	221.0 / NA	70	*238* (TAG→TAA)	ybhM	BAX Inhibitor‑1 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/35);  total (35/35)

AGTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATA  >  NC_000913/821373‑821629                                                                                                                                      |                                                                                                                            agTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        >  1:952954/1‑139 (MQ=255) agTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        <  1:1087777/139‑1 (MQ=255)      tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   <  1:896407/139‑1 (MQ=255)      tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   <  2:952954/139‑1 (MQ=255)      tgGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   >  1:899106/1‑139 (MQ=255)       ggAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  2:899106/139‑1 (MQ=255)         aGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcaca                                                                                                                <  1:11401/139‑1 (MQ=255)                   gTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGaa                                                                                                      <  2:143841/139‑1 (MQ=255)                        gcTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTaa                                                                                                 <  1:788938/139‑1 (MQ=255)                                               ctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  >  1:1169234/1‑99 (MQ=255)                                               ctATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  2:1169234/99‑1 (MQ=255)                                                  ttACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTaa                                                                                                          >  1:690635/1‑104 (MQ=255)                                                  ttACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTaa                                                                                                          <  2:690635/104‑1 (MQ=255)                                                      tATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCg                                                                   >  2:1081928/1‑139 (MQ=255)                                                        ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  >  2:397103/1‑90 (MQ=255)                                                        ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  1:397103/90‑1 (MQ=255)                                                        ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTa                                                                                              <  1:875127/110‑1 (MQ=255)                                                        ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTa                                                                                              >  2:875127/1‑110 (MQ=255)                                                        ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGcc                                                                 >  1:495287/1‑139 (MQ=255)                                                        ttttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGcc                                                                 >  1:860508/1‑139 (MQ=255)                                                          ttATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCg                                                               >  2:52741/1‑139 (MQ=255)                                                            aTCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTa                                                             >  2:1141022/1‑139 (MQ=255)                                                                gCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        <  1:356451/76‑1 (MQ=255)                                                                gCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTa                                                                                                                        >  2:356451/1‑76 (MQ=255)                                                                  ttcttTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGcttct                                                       >  1:1179456/1‑139 (MQ=255)                                                                       tGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGaa                                                                                                      <  2:745027/87‑1 (MQ=255)                                                                       tGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGaa                                                                                                      >  1:745027/1‑87 (MQ=255)                                                                          gATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTgg                                               <  2:379679/139‑1 (MQ=255)                                                                           aTACTTGGCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCCTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCACGTAGCTTCTTCGCCTGGc                                              >  2:847696/1‑139 (MQ=255)                                                                              cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcaca                                                                                                                >  1:892406/1‑70 (MQ=255)                                                                              cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGcaca                                                                                                                <  2:892406/70‑1 (MQ=255)                                                                              cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   <  1:736828/67‑1 (MQ=255)                                                                              cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGc                                                                                                                   >  2:736828/1‑67 (MQ=255)                                                                              cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCttt                                           >  1:424658/1‑139 (MQ=255)                                                                              cTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTACACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCttt                                           >  2:200626/1‑139 (MQ=255)                                                                               ttGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  >  1:185197/1‑67 (MQ=255)                                                                               ttGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  2:185197/67‑1 (MQ=255)                                                                                 gccagacaaGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCa                                        >  1:640557/9‑139 (MQ=255)                                                                                 gCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACt                                                                                                    <  1:800801/79‑1 (MQ=255)                                                                                 gCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACt                                                                                                    >  2:800801/1‑79 (MQ=255)                                                                                 gCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCa                                        >  1:583738/1‑139 (MQ=255)                                                                                   cgcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  2:420435/63‑1 (MQ=255)                                                                                   cgcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  >  1:420435/1‑63 (MQ=255)                                                                                    gcgATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTACACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCa                                     <  1:200626/139‑1 (MQ=255)                                                                                       aTAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  <  2:1179456/139‑1 (MQ=255)                                                                                       aTAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACa                                  <  2:860508/139‑1 (MQ=255)                                                                                         aGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  1:239644/57‑1 (MQ=255)                                                                                         aGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  >  2:239644/1‑57 (MQ=255)                                                                                         aGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAgg                                >  2:775124/1‑139 (MQ=255)                                                                                           cTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTaaa                                                                                                                      >  1:94155/1‑51 (MQ=255)                                                                                           cTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTaaa                                                                                                                      <  2:94155/51‑1 (MQ=255)                                                                                           cTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATc                                                                                   >  2:263357/1‑86 (MQ=255)                                                                                           cTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATc                                                                                   <  1:263357/86‑1 (MQ=255)                                                                                             ataACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  <  1:985764/53‑1 (MQ=255)                                                                                             ataACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGca                                                                                                                  >  2:985764/1‑53 (MQ=255)                                                                                                aCGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGa                         <  1:52741/139‑1 (MQ=255)                                                                                                    ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCg                                                                                                                          >  2:1227700/1‑38 (MQ=255)                                                                                                    ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCg                                                                                                                          <  1:1227700/38‑1 (MQ=255)                                                                                                    ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACt                                                                                               <  2:1127076/65‑1 (MQ=255)                                                                                                    ttGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACt                                                                                               >  1:1127076/1‑65 (MQ=255)                                                                                                     tGTCTGGCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAgc                    >  2:242459/1‑139 (MQ=255)                                                                                                           gCAAAGGCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCcagc              <  2:495287/139‑1 (MQ=255)                                                                                                                 gCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCCCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCa                                     <  1:846002/110‑1 (MQ=255)                                                                                                                 gCATACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACACCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCa                                     >  2:846002/1‑110 (MQ=255)                                                                                                                   aTACGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTgg      <  2:424658/139‑1 (MQ=255)                                                                                                                     aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGaa                                                                                                      >  2:87149/1‑41 (MQ=255)                                                                                                                     aCGCGTTTTTTTCATTAACCGTAAATGCACATACTAAAGaa                                                                                                      <  1:87149/41‑1 (MQ=255)                                                                                                                       gcgTTTTTTTCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATa  <  2:583738/139‑1 (MQ=255)                                                                                                                           ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTc                                          >  2:216701/1‑95 (MQ=255)                                                                                                                           ttttttCATTAACCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTc                                          <  1:216701/95‑1 (MQ=255)                                                                                                                                      |                                                                                                                            AGTTGTGGAGTCCGGTTCGTTGCGCTTTTACAGGTGCATTAACACTCTATTACTATTTTATCGGCTTCTTTGGGATACTTGCCGCGATAGCTATAACGCTTGTCTGGCAAAGGCATACGCGTTTTTTTCATTAGCCGTAAATGCACATACTAAAGAACTTAACTAAACTTCACATCGCCGCTTCATTTTTCGCCCGTAGCTTCTTCGCCTGGCTTTCCAGCAACAGGTAACAGATCAGCGCCAGCAGCAGTGGGATA  >  NC_000913/821373‑821629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑