Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	825,102	G→A	A336V (GCG→GTG)	ybhR ←	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	825,102	0	G	A	100.0%	183.7 / NA	58	A336V (GCG→GTG)	ybhR	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/27);  total (31/27)

CCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCA  >  NC_000913/824966‑825234                                                                                                                                         |                                                                                                                                     ccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTcaca                                                                                                                                    <  2:1277106/139‑1 (MQ=255)                  gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      <  2:28006/136‑1 (MQ=255)                  gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      >  1:28006/1‑136 (MQ=255)                    tGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCt                                                                                                                 <  2:1132326/139‑1 (MQ=255)                                      tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGt                                                                                               >  2:1124884/1‑139 (MQ=255)                                        cATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                                                             <  1:1124884/139‑1 (MQ=255)                                         aTCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGc                                                                                            <  2:527421/139‑1 (MQ=255)                                            aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAAACTTCCTGGTAATGTCCGTAAAGTGGCGaa                                                                                         <  1:916867/139‑1 (MQ=255)                                                        aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCt                                                                                                                                <  1:1000439/88‑1 (MQ=255)                                                        aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCt                                                                                                                                >  2:1000439/1‑88 (MQ=255)                                                        aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATc                                                                                                                     <  1:1040781/99‑1 (MQ=255)                                                        aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATc                                                                                                                     >  2:1040781/1‑99 (MQ=255)                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACCCAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTc                                                                        >  2:880766/1‑139 (MQ=255)                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                        >  2:607609/1‑123 (MQ=255)                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                        <  1:607609/123‑1 (MQ=255)                                                               cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      <  2:20537/91‑1 (MQ=255)                                                               cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      >  1:20537/1‑91 (MQ=255)                                                               cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCACATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAg                                                                      >  1:848411/1‑139 (MQ=255)                                                                           aCCCTGTCGTGGCCGTTATTACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAGATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTCGCAGCCa                                                          <  2:848411/139‑1 (MQ=255)                                                                                gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                             <  1:566390/83‑1 (MQ=255)                                                                                gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                             >  2:566390/1‑83 (MQ=255)                                                                                gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                         >  2:1041830/1‑103 (MQ=255)                                                                                gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                         <  1:1041830/103‑1 (MQ=255)                                                                                gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCg                                                     >  2:1157683/1‑139 (MQ=255)                                                                                gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCg                                                     >  1:419685/1‑139 (MQ=255)                                                                                      gCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      >  2:1073236/1‑68 (MQ=255)                                                                                      gCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      <  1:1073236/68‑1 (MQ=255)                                                                                       ccGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                        <  1:626549/97‑1 (MQ=255)                                                                                       ccGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                        >  2:626549/1‑97 (MQ=255)                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                             <  2:17405/72‑1 (MQ=255)                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                             >  1:17405/1‑72 (MQ=255)                                                                                            aTCACCAGTAGCGGCCACAAACTATTCCAGACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      >  2:458595/1‑62 (MQ=255)                                                                                            aTCACCAGTAGCGGCCACAAACTATTCCAGACAATATCCAGACTCACATCCTTCaaataaat                                                                                                                      <  1:458595/62‑1 (MQ=255)                                                                                                ccAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                             >  1:131032/1‑67 (MQ=255)                                                                                                ccAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGt                                                                                                             <  2:131032/67‑1 (MQ=255)                                                                                                  aGTAGCGGCCACAAACTATTCCACACAATATCCAGACTAACATCCTTCAAATAAATCTGATTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGgag                                   >  2:417442/1‑139 (MQ=255)                                                                                                   gTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTaa                                                                                                           >  2:242574/1‑66 (MQ=255)                                                                                                   gTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTaa                                                                                                           <  1:242574/66‑1 (MQ=255)                                                                                                    tAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCt                                                                                                                                <  2:931805/44‑1 (MQ=255)                                                                                                    tAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCt                                                                                                                                >  1:931805/1‑44 (MQ=255)                                                                                                       cGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGc                                                                                                                  <  1:587036/55‑1 (MQ=255)                                                                                                       cGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGc                                                                                                                  >  2:587036/1‑55 (MQ=255)                                                                                                        ggCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                        >  2:108410/1‑80 (MQ=255)                                                                                                        ggCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                        <  1:108410/80‑1 (MQ=255)                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTc                                           >  1:697579/1‑124 (MQ=255)                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTc                                           <  2:697579/124‑1 (MQ=255)                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTa                            >  2:639524/1‑139 (MQ=255)                                                                                                           cacaAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATg                                                                                                         >  2:273165/1‑60 (MQ=255)                                                                                                           cacaAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATg                                                                                                         <  1:273165/60‑1 (MQ=255)                                                                                                                        acacaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAAt             >  1:818986/1‑139 (MQ=255)                                                                                                                         cacaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                         <  1:670468/62‑1 (MQ=255)                                                                                                                         cacaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                         >  2:670468/1‑62 (MQ=255)                                                                                                                          acaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCa                                                          >  1:1149352/1‑92 (MQ=255)                                                                                                                          acaATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCa                                                          <  2:1149352/92‑1 (MQ=255)                                                                                                                            aaTATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCg         >  2:71825/1‑139 (MQ=255)                                                                                                                               atCCAGACTCACATCCGTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAATCGGAAAGGAGAATGGCCGGc      <  1:880766/139‑1 (MQ=255)                                                                                                                                   aGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcatca  >  2:786676/1‑139 (MQ=255)                                                                                                                                      cTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                      >  2:1007213/1‑116 (MQ=255)                                                                                                                                      cTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                      <  1:1007213/116‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCA  >  NC_000913/824966‑825234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑