Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 850,389 T→C intergenic (‑292/‑61) rhtA ← / → ompX threonine and homoserine efflux system/outer membrane protein X

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,3890TC100.0% 117.7 / NA 35intergenic (‑292/‑61)rhtA/ompXthreonine and homoserine efflux system/outer membrane protein X
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

GATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCT  >  NC_000913/850253‑850518
                                                                                                                                        |                                                                                                                                 
gATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTccc                                                                                                                                 >  1:1007129/1‑139 (MQ=255)
                                      ttCTCTGAAAACGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTa                                                                                                            <  1:776068/122‑1 (MQ=255)
                                      tcctctGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTACCCCATTAATTTTTGATATATTTa                                                                                                            >  2:776068/3‑122 (MQ=255)
                                                     aTTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   <  2:36552/100‑1 (MQ=255)
                                                     aTTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   >  1:36552/1‑100 (MQ=255)
                                                                    gATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                       >  2:1272429/1‑113 (MQ=255)
                                                                    gATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                       <  1:1272429/113‑1 (MQ=255)
                                                                           gtgtATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATg                                                      <  2:5997/139‑1 (MQ=255)
                                                                           gtgtATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATg                                                      >  1:5997/1‑139 (MQ=255)
                                                                                tGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCttt                                                 >  2:1015777/1‑139 (MQ=255)
                                                                                      cGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTa                                                                                                            <  1:862141/74‑1 (MQ=255)
                                                                                      cGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTa                                                                                                            >  2:862141/1‑74 (MQ=255)
                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   >  1:1164288/1‑65 (MQ=255)
                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   <  2:1164288/65‑1 (MQ=255)
                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATc                                                                                      >  2:76714/1‑94 (MQ=255)
                                                                                        ttCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATc                                                                                      <  1:76714/94‑1 (MQ=255)
                                                                                                cATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   >  1:107835/1‑57 (MQ=255)
                                                                                                cATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   <  2:107835/57‑1 (MQ=255)
                                                                                                       tGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   <  2:38192/50‑1 (MQ=255)
                                                                                                       tGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   >  2:937989/1‑50 (MQ=255)
                                                                                                       tGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   <  1:937989/50‑1 (MQ=255)
                                                                                                       tGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGat                                                                                                                   >  1:38192/1‑50 (MQ=255)
                                                                                                       tGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACt                                                                                                <  2:1121518/69‑1 (MQ=255)
                                                                                                       tGGCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACt                                                                                                >  1:1121518/1‑69 (MQ=255)
                                                                                                         gCCCGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCttt                        <  1:497989/139‑1 (MQ=255)
                                                                                                          cccGTTAGATATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                       <  2:883892/75‑1 (MQ=255)
                                                                                                          cccGTTAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAAt                                                                                       >  1:883892/1‑75 (MQ=255)
                                                                                                              ttAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGatat                                                                                                                 <  2:1141765/45‑1 (MQ=255)
                                                                                                              ttAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGatat                                                                                                                 >  1:1141765/1‑45 (MQ=255)
                                                                                                              ttAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTAt                                                                                             >  1:682585/1‑65 (MQ=255)
                                                                                                              ttAGACATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTAt                                                                                             <  2:682585/65‑1 (MQ=255)
                                                                                                                   cATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCt                          <  1:911934/127‑1 (MQ=255)
                                                                                                                   cATCACAAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCt                          >  2:911934/1‑127 (MQ=255)
                                                                                                                      cacaAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGa                                                                                                  >  1:937125/1‑52 (MQ=255)
                                                                                                                      cacaAATCGCGAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGa                                                                                                  <  2:937125/52‑1 (MQ=255)
                                                                                                                               cgAAGAGTTCCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCt  <  2:1007129/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCT  >  NC_000913/850253‑850518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: