Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 855,796 A→G intergenic (‑52/‑167) ldtB ← / → ybiT L,D‑transpeptidase linking Lpp to murein/ABC‑F family putative regulatory ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913855,7960AG100.0% 92.2 / NA 27intergenic (‑52/‑167)ldtB/ybiTL,D‑transpeptidase linking Lpp to murein/ABC‑F family putative regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

TAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGC  >  NC_000913/855665‑855931
                                                                                                                                   |                                                                                                                                       
tAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  <  1:1189374/139‑1 (MQ=255)
        cGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat                                                                                                                          >  2:19746/1‑139 (MQ=255)
        cGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATtat                                                                                                                          <  1:19746/139‑1 (MQ=255)
                        cAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTc                                                                                                          <  2:843553/139‑1 (MQ=255)
                         aaaaGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTca                                                                                                         >  2:345087/1‑139 (MQ=255)
                             gCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGt                                                                                                     >  1:1126394/1‑139 (MQ=255)
                                     caGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg                                                                                             <  2:1126394/139‑1 (MQ=255)
                                                                      tCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCt                                                            <  1:854539/139‑1 (MQ=255)
                                                                       cATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt                                                           <  2:358515/139‑1 (MQ=255)
                                                                              aTGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTa                                                    >  1:685105/1‑139 (MQ=255)
                                                                                   aaGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  <  1:1069131/56‑1 (MQ=255)
                                                                                   aaGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAg                                                                                                                                  >  2:1069131/1‑56 (MQ=255)
                                                                                      cGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc                                                                                                                               <  2:1282109/56‑1 (MQ=255)
                                                                                      cGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc                                                                                                                               >  1:1282109/1‑56 (MQ=255)
                                                                                                tgCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc                                                                                                                               <  2:602768/46‑1 (MQ=255)
                                                                                                tgCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAgcgc                                                                                                                               >  1:602768/1‑46 (MQ=255)
                                                                                                     ggCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt                                                           <  1:380499/109‑1 (MQ=255)
                                                                                                     ggCAGGCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCtt                                                           >  2:380499/1‑109 (MQ=255)
                                                                                                          gCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGAtc                             <  1:265934/134‑1 (MQ=255)
                                                                                                          gCCATTGGGTTGAGAATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGAtc                             >  2:265934/1‑134 (MQ=255)
                                                                                                                     gagaATATTAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATg             >  2:295578/1‑139 (MQ=255)
                                                                                                                             tAGAGTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATg     >  2:1060002/1‑139 (MQ=255)
                                                                                                                               nagNNNNGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcg   <  2:452664/138‑1 (MQ=255)
                                                                                                                               gagTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcg   <  1:345087/139‑1 (MQ=255)
                                                                                                                               gagTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcg   <  1:194832/139‑1 (MQ=255)
                                                                                                                                agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATGAGATGTGTACGAAATGACATTATTTGCCTTTGGCTTGAGTGTAGACGTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATgcgc  >  2:754272/1‑139 (MQ=255)
                                                                                                                                agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg                                                                                             <  1:140014/48‑1 (MQ=255)
                                                                                                                                agTGTTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAg                                                                                             >  2:140014/1‑48 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TAAGTTACCGCAGAGGCGGTACTGCAAAAGCCGACAACAGCGAAGGCCGCTGCGAATAATGTTTTCAATTTCATATTCATGTTAAGCGAGATTTTGTGCCTGGCAGGCCATTGGGTTGAGAATATTAGAGTATTGGAAGCGCATTATAAGTTCATTCCAGCTCACAGTGAAATCAGATGTGTACGAAATCACATTTTTTGCCTTTGGCTTGAGTGTAGACCTTAAGCGAGGAGCAGGATCTTCTTTCAGACTTATGGCATAATGCGC  >  NC_000913/855665‑855931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: