| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 922,366 | C→T | *75* (TAG→TAA) | cspD ← | inhibitor of DNA replication, cold shock protein homolog |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 922,366 | 0 | C | T | 97.8% | 138.5 / ‑6.9 | 46 | *75* (TAG→TAA) | cspD | inhibitor of DNA replication, cold shock protein homolog |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (25/20); minor base A (1/0); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGTGGAAT > NC_000913/922243‑922499 | gCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCt > 1:1167330/1‑139 (MQ=255) aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg < 2:10871/139‑1 (MQ=255) tAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg > 2:991791/1‑139 (MQ=255) gCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGAc < 2:598292/117‑1 (MQ=255) gCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGAc > 1:598292/1‑117 (MQ=255) ggCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGcc > 1:315148/1‑139 (MQ=255) agTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGcc > 2:333056/1‑139 (MQ=255) gTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGATATGCTACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCtttttaccc > 1:583390/1‑134 (MQ=255) agaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggt > 1:1192547/1‑134 (MQ=255) agaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggt < 2:1192547/134‑1 (MQ=255) agaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACa > 1:1079460/1‑139 (MQ=255) aGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGc > 2:575382/1‑68 (MQ=255) aGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGc < 1:575382/68‑1 (MQ=255) tGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCtt > 1:1281078/1‑139 (MQ=255) ggCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGCGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGa < 1:689123/122‑1 (MQ=255) ggCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGa > 2:689123/1‑122 (MQ=255) tttAGGATGTACACAATGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTAGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGt < 2:980646/139‑1 (MQ=255) aggagaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGAAATCAAACTGAAGGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa > 2:777038/3‑139 (MQ=255) aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGc < 1:522449/45‑1 (MQ=255) aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGc > 2:522449/1‑45 (MQ=255) aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa < 1:784955/139‑1 (MQ=255) aTGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTAGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa > 2:784955/1‑139 (MQ=255) tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTc < 1:556345/120‑1 (MQ=255) tGAGACAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTc > 2:556345/1‑120 (MQ=255) gagaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtg < 1:903941/139‑1 (MQ=255) gagaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAAtg < 2:1281078/139‑1 (MQ=255) gaCAGCAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACaa > 1:890118/1‑45 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg > 2:289730/1‑40 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg > 2:724017/1‑40 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg < 1:289730/40‑1 (MQ=39) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACggg < 1:724017/40‑1 (MQ=39) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACaa < 2:890118/45‑1 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggtgg < 2:465393/80‑1 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggtgg > 1:465393/1‑80 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGt < 2:340421/115‑1 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGt > 1:340421/1‑115 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTaa < 1:527159/122‑1 (MQ=255) gaCAGAAGAGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTaa > 2:527159/1‑122 (MQ=255) aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGa < 2:1149142/92‑1 (MQ=255) aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGa > 1:1149142/1‑92 (MQ=255) aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa < 2:1133500/130‑1 (MQ=255) aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa > 1:1133500/1‑130 (MQ=255) aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa > 2:909572/1‑130 (MQ=255) aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGaa < 1:909572/130‑1 (MQ=255) aagaGTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGTGGaat > 2:616946/1‑139 (MQ=255) gagTTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAAcca > 1:251409/1‑118 (MQ=255) | GCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTTTTAGCGTTCTGTAACCATCCATCTGAATGGTGGAAT > NC_000913/922243‑922499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |