Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 947,123 T→C intergenic (+104/‑106) ycaD → / → ycaM putative MFS transporter, inner membrane protein/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913947,1230TC100.0% 105.2 / NA 30intergenic (+104/‑106)ycaD/ycaMputative MFS transporter, inner membrane protein/putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

GTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGG  >  NC_000913/946988‑947247
                                                                                                                                       |                                                                                                                            
gTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgca                                                                                                                           >  2:894541/1‑139 (MQ=255)
          gAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag                                                                                                                          >  2:742028/1‑130 (MQ=255)
          gAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag                                                                                                                          <  1:742028/130‑1 (MQ=255)
             aCCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTta                                                                                                                   >  2:873497/1‑134 (MQ=255)
             aCCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTta                                                                                                                   <  1:873497/134‑1 (MQ=255)
                        aCGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGc                                                                                                   <  1:894541/139‑1 (MQ=255)
                                                  tgAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCcgcg                                                                         >  2:562004/1‑139 (MQ=255)
                                                    aaCTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCa                                                                       <  1:562004/139‑1 (MQ=255)
                                                         aCTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCg                                                                                                    <  1:340105/105‑1 (MQ=255)
                                                         aCTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCg                                                                                                    >  2:340105/1‑105 (MQ=255)
                                                            gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag                                                                                                                          <  1:1140308/80‑1 (MQ=255)
                                                            gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag                                                                                                                          >  2:1140308/1‑80 (MQ=255)
                                                            gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCcgc                                                                          >  2:922352/1‑128 (MQ=255)
                                                            gTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCcgc                                                                          <  1:922352/128‑1 (MQ=255)
                                                                       tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcagc                                                                                                                         <  2:955177/70‑1 (MQ=255)
                                                                       tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcagc                                                                                                                         >  1:955177/1‑70 (MQ=255)
                                                                       tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag                                                                                                                          <  2:393578/69‑1 (MQ=255)
                                                                       tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCgcag                                                                                                                          >  1:393578/1‑69 (MQ=255)
                                                                       tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATc                                                                  <  1:935844/125‑1 (MQ=255)
                                                                       tGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATc                                                                  >  2:935844/1‑125 (MQ=255)
                                                                                               cTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGAt                                                   <  2:1286241/116‑1 (MQ=255)
                                                                                               cTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGAt                                                   >  1:1286241/1‑116 (MQ=255)
                                                                                                ttGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGAt                           <  2:316898/139‑1 (MQ=255)
                                                                                                  gCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCg                                                                                           <  2:358639/73‑1 (MQ=255)
                                                                                                  gCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCg                                                                                           >  1:358639/1‑73 (MQ=255)
                                                                                                    tttATTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGt                       >  1:1093656/1‑139 (MQ=255)
                                                                                                       aTTCCAAATTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTa                    <  2:1093656/139‑1 (MQ=255)
                                                                                                             aaTTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGAt                           <  2:487970/126‑1 (MQ=255)
                                                                                                             aaTTTTATTCGTTTAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGAt                           >  1:487970/1‑126 (MQ=255)
                                                                                                                         ttAAAAATAAAATGCGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAgg  >  1:975263/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                            
GTCATACGCCGAAACCCGTTGCTCACGTGTAAATGAATTCAAGCAGAGTGTGAACTTACTGTTTCACACTCTGCTTTTTTGTTTCTTCTATCTGACTTGCTTTATTCCAAATTTTATTCGTTTAAAAATAAAATGTGCAGCAGGTTATAATTTTGCATTTCGCTATTTCCGCACTTCTTATTTGCCGCGCATAATCCCTCGTTTTACCGATGCCCCTTTAATTTTGGCGAAGGATTTGTCTATGGCTGGGAATGTTCAGG  >  NC_000913/946988‑947247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: