Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,054,899	A→G	V427A (GTG→GCG)	torS ←	hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,054,899	0	A	G	100.0%	72.0 / NA	21	V427A (GTG→GCG)	torS	hybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

TGAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCACCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGG  >  NC_000913/1054779‑1055010                                                                                                                         |                                                                                                                tGAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAg                                                                                               <  1:411326/139‑1 (MQ=255)  gAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGc                                                                                              <  2:482824/139‑1 (MQ=255)   aGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCt                                                                                             >  2:695138/1‑139 (MQ=255)       gTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTAc                                                                                         <  1:118775/139‑1 (MQ=255)       gTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTAc                                                                                         >  1:422734/1‑139 (MQ=255)       gTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTAc                                                                                         <  1:695138/139‑1 (MQ=255)                     gTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTgc                                                                           <  2:1191569/139‑1 (MQ=255)                         aGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGcgccg                                                                       >  2:908532/1‑139 (MQ=255)                         aGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGcgccg                                                                       >  2:133083/1‑139 (MQ=255)                           cGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCgccgcc                                                                     >  1:256157/1‑139 (MQ=255)                              tgtgCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGc                                                                  >  1:462551/1‑139 (MQ=255)                                        tcATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGt                                                        <  1:133083/139‑1 (MQ=255)                                          aTGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTgg                                                      >  1:149117/1‑139 (MQ=255)                                          aTGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTgg                                                      <  2:422734/139‑1 (MQ=255)                                                 aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCgg                                                         <  2:1094985/129‑1 (MQ=255)                                                 aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCgg                                                         >  1:1094985/1‑129 (MQ=255)                                                  tCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGCTCGCGGTGGCGATTCAg                                              <  1:1260672/139‑1 (MQ=255)                                                     ccgccAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGc                                           <  2:462551/139‑1 (MQ=255)                                                                                        tCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTcgc                                                           >  1:653883/1‑88 (MQ=255)                                                                                        tCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTcgc                                                           <  2:653883/88‑1 (MQ=255)                                                                                              tCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATgg  <  1:559574/139‑1 (MQ=255)                                                                                                                         |                                                                                                                TGAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCACCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGG  >  NC_000913/1054779‑1055010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑