Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,204,160	G→A	*113* (TAG→TAA)	ymfM →	e14 prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,204,160	0	G	A	100.0%	135.9 / NA	43	*113* (TAG→TAA)	ymfM	e14 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/25);  total (18/25)

AAGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCA  >  NC_000913/1204026‑1204292                                                                                                                                       |                                                                                                                                     aaGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                  <  1:128944/139‑1 (MQ=255) aaGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                  <  1:964378/139‑1 (MQ=255) aaGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                  <  1:933653/139‑1 (MQ=255)           cGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAg                                                                                                                        >  1:948078/1‑139 (MQ=255)               gTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACt                                                                                                                    >  1:1021676/1‑139 (MQ=255)                tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTa                                                                                                                   >  1:366519/1‑139 (MQ=255)                   ccGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                       <  2:975619/132‑1 (MQ=255)                   ccGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                       >  1:975619/1‑132 (MQ=255)                        agagTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCAt                                                                                                                           <  2:493697/123‑1 (MQ=255)                        agagTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCAt                                                                                                                           >  1:493697/1‑123 (MQ=255)                          agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTCAATTAACCgg                                                                                                         <  2:954218/139‑1 (MQ=255)                          agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                         <  2:948078/139‑1 (MQ=255)                            tCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                       <  1:1156619/139‑1 (MQ=255)                                             gAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgc                                                                                      <  1:892281/139‑1 (MQ=255)                                                        ggTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                         >  2:15679/1‑109 (MQ=255)                                                        ggTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                         <  1:15679/109‑1 (MQ=255)                                                                       cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAg                                                            >  1:704449/1‑139 (MQ=255)                                                                                    cATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                  >  2:630877/1‑56 (MQ=255)                                                                                    cATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                  <  1:630877/56‑1 (MQ=255)                                                                                      tGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGc                                             >  2:1198600/1‑139 (MQ=255)                                                                                      tGAATGCTTCAGCCCGCTCGAACAGTCTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAg                                                            >  1:623546/1‑124 (MQ=255)                                                                                      tGAATGCTTCAGCCCGCTCGAACAGTCTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAg                                                            <  2:623546/124‑1 (MQ=255)                                                                                       gAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTgg                                                       <  1:409160/128‑1 (MQ=255)                                                                                       gAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTgg                                                       >  2:409160/1‑128 (MQ=255)                                                                                                  cccGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGa                                 >  2:660588/1‑139 (MQ=255)                                                                                                   cctcTCGAATATTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGAt                                <  2:1122290/136‑1 (MQ=255)                                                                                                      cTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgcg                                                                                     >  1:502864/1‑83 (MQ=255)                                                                                                      cTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgcg                                                                                     <  2:502864/83‑1 (MQ=255)                                                                                                      cTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCa                                                                         >  1:1249283/1‑95 (MQ=255)                                                                                                      cTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCa                                                                         <  2:1249283/95‑1 (MQ=255)                                                                                                               tttCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATc                                                               <  2:976748/96‑1 (MQ=255)                                                                                                               tttCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATc                                                               >  1:976748/1‑96 (MQ=255)                                                                                                                ttCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACg                   <  2:1021676/139‑1 (MQ=255)                                                                                                                ttCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACg                   <  1:31073/139‑1 (MQ=255)                                                                                                                    tcgtAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAgg               <  2:704449/139‑1 (MQ=255)                                                                                                                     cgtAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGgt              <  1:660588/139‑1 (MQ=255)                                                                                                                       tAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGgt              >  2:634951/1‑137 (MQ=255)                                                                                                                       tAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGgt              <  1:634951/137‑1 (MQ=255)                                                                                                                              aGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGcc                                        >  2:1198601/1‑104 (MQ=255)                                                                                                                              aGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGcc                                        <  1:1198601/104‑1 (MQ=255)                                                                                                                                ggAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATc                                                                              >  2:135324/1‑64 (MQ=255)                                                                                                                                ggAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATc                                                                              <  1:135324/64‑1 (MQ=255)                                                                                                                                 gAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCa  <  1:1077691/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     AAGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCA  >  NC_000913/1204026‑1204292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑