Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,205,731	G→A	*61* (TAG→TAA)  pseudogene (1/412 nt)	ymfR → beeE →	e14 prophage, uncharacterized protein pseudogene, portal protein family, e14 prophage,Phage or Prophage Related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,205,731	0	G	A	100.0%	57.6 / NA	19	*61* (TAG→TAA)  pseudogene (1/412 nt)	ymfR beeE	e14 prophage, uncharacterized protein pseudogene, portal protein family, e14 prophage,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

CCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCT  >  NC_000913/1205649‑1205856                                                                                   |                                                                                                                              ccGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCcagca                                                                       >  2:1098094/1‑139 (MQ=255)     ggCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGc                                                                   >  2:599061/1‑139 (MQ=255)                 gTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCa                                                       <  1:1098094/139‑1 (MQ=255)                    cTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCg                                                    <  1:418393/139‑1 (MQ=255)                     tGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCgg                                                   <  2:937048/139‑1 (MQ=255)                      ggTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCggg                                                  <  1:995603/139‑1 (MQ=255)                      ggTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCggg                                                  <  1:599061/139‑1 (MQ=255)                       gTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGc                                                 >  2:1163936/1‑139 (MQ=255)                                 gtggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGa                                       <  1:290341/139‑1 (MQ=255)                                  tggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                               >  2:821929/1‑130 (MQ=255)                                  tggCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGACGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                               <  1:821929/130‑1 (MQ=255)                                                     tACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCAc                                                                              <  1:758784/80‑1 (MQ=255)                                                     tACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCAc                                                                              >  2:758784/1‑80 (MQ=255)                                                     tACACAGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGa                   >  1:1246439/1‑139 (MQ=255)                                                          aGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATcagc              <  2:1246439/139‑1 (MQ=255)                                                          aGTCGTCTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATcagc              <  2:234777/139‑1 (MQ=255)                                                               tcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                               <  1:626638/101‑1 (MQ=255)                                                               tcTGTCGGCGGAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTg                                               >  2:626638/1‑101 (MQ=255)                                                                      gcggAGGTAAATAATGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCt  >  2:31898/1‑139 (MQ=255)                                                                                   |                                                                                                                              CCGGGGCGCTGTGCCTGTTCTGGTCGTGGCTGGTGGCGCGATATCTCGACCGTACACAGTCGTCTGTCGGCGGAGGTAAATAGTGTTCTTTTCGGGATTATTTCAACGAAAAAGTGACGCACCGGTGACCACGCCAGCAGAGCTGGCGGATGCCATCGGGCTGTCGTATGACACCTATACCGGAAAGCAGATCAGCAGTCAGCGGGCT  >  NC_000913/1205649‑1205856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑