Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,271,455 G→A T132I (ACC→ATC)  chaA ← calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,271,4550GA100.0% 130.3 / NA 42T132I (ACC→ATC) chaAcalcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/19);  total (23/19)

ATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCG  >  NC_000913/1271320‑1271565
                                                                                                                                       |                                                                                                              
aTTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAAAAGATTCATATATTGGATgg                                                                                                             >  1:540651/1‑139 (MQ=255)
 ttAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                            <  1:296638/139‑1 (MQ=255)
                    cTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaac                                                                                         >  2:561190/1‑139 (MQ=255)
                                          gcaggcagAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGcc                                                                   >  2:201598/1‑139 (MQ=255)
                                                     cATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACa                                                        <  1:201598/139‑1 (MQ=255)
                                                          gAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCAt                                                   <  2:540651/139‑1 (MQ=255)
                                                                 cAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGAtt                                            <  2:11686/139‑1 (MQ=255)
                                                                 cAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGAtt                                            >  1:11686/1‑139 (MQ=255)
                                                                    tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                            <  1:473699/88‑1 (MQ=255)
                                                                    tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGccc                                                                                            >  2:473699/1‑88 (MQ=255)
                                                                    tACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGaa                                         >  2:487941/1‑139 (MQ=255)
                                                                         ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCGAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                          >  1:807216/1‑117 (MQ=255)
                                                                         ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                        <  2:674285/103‑1 (MQ=255)
                                                                         ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAAc                                                                        >  1:674285/1‑103 (MQ=255)
                                                                         ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCAc                                                                >  2:992039/1‑111 (MQ=255)
                                                                         ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCAc                                                                <  1:992039/111‑1 (MQ=255)
                                                                         ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTat                                                          <  2:807216/117‑2 (MQ=255)
                                                                         ttATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATagag                                    <  1:66054/139‑1 (MQ=255)
                                                                                 aGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCa                             <  2:1099259/138‑1 (MQ=255)
                                                                                 aGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCa                             >  1:1099259/1‑138 (MQ=255)
                                                                                   ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                          >  1:353325/1‑75 (MQ=255)
                                                                                   ggggAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCcaa                                                                                          <  2:353325/75‑1 (MQ=255)
                                                                                             gcAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                            <  1:368443/47‑1 (MQ=255)
                                                                                             gcAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGc                                                                                                            >  2:368443/1‑47 (MQ=255)
                                                                                                 ttAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                          <  1:177663/93‑1 (MQ=255)
                                                                                                 ttAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTaa                                                          >  2:177663/1‑93 (MQ=255)
                                                                                                 ttAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCa                             >  2:1016677/1‑122 (MQ=255)
                                                                                                 ttAAATACTGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCa                             <  1:1016677/122‑1 (MQ=255)
                                                                                                         tGCTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGc    >  2:978067/1‑139 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATg                                                                                  >  2:658885/1‑59 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATg                                                                                  <  1:658885/59‑1 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATaa                                                 >  2:37036/1‑92 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATaa                                                 <  1:37036/92‑1 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATg                                               <  2:215329/94‑1 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATg                                               >  1:215329/1‑94 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGaa                                         >  1:550092/1‑100 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGaa                                         <  2:550092/100‑1 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATc                              <  2:733661/111‑1 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATc                              >  1:733661/1‑111 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCTCGGCGTCGCCg  >  1:487925/1‑139 (MQ=255)
                                                                                                           cTTGATACCAAACAGATTCATATATTGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCg  >  2:1144871/1‑139 (MQ=255)
                                                                                                                                    tGGATGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGt                                                            >  1:605453/1‑56 (MQ=255)
                                                                                                                                       |                                                                                                              
ATTAATGCTACCAGTAACGCCTGACCGGTTGAAAAATTCGCCGCAGGCAGAGCCATTGGAAATACCAGTACGATTATCGCCAGGGGGAACAGCGCAATTAAATACTGCTTGATACCAAACAGATTCATATATTGGGTGGCAAACTTACGACCGCCCAACAATAATGAAAAGCCAACCAGCCCACCGGTAACAATCATAATGATTGAATAGAGCGTATCACGCATTAGCGTTGGCGCGGCGTCGCCG  >  NC_000913/1271320‑1271565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑