Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,458,617 A→G A118A (GCA→GCG)  paaG → 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,458,6170AG100.0% 138.2 / NA 39A118A (GCA→GCG) paaG1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/17);  total (22/17)

CCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCG  >  NC_000913/1458488‑1458741
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ccccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGc                                                                                                                     >  1:816333/1‑139 (MQ=255)
  ccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                                                                   >  1:155364/1‑139 (MQ=255)
   cGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                  >  1:475125/1‑139 (MQ=255)
               aGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                                                                <  1:1204909/129‑1 (MQ=255)
               aGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                                                                >  2:1204909/1‑129 (MQ=255)
               aGGGATGTCAGTTGAACGCTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATtgct                                                                                                      <  1:871212/139‑1 (MQ=255)
                     gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGt                                                                                                >  1:1063174/1‑139 (MQ=255)
                             aaCGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCaaa                                                                                        <  1:168549/139‑1 (MQ=255)
                                  ttCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCg                                                                                   >  2:749353/1‑139 (MQ=255)
                                   tCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGt                                                                                  >  2:86379/1‑139 (MQ=255)
                                     tataACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAAGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                <  1:863898/139‑1 (MQ=255)
                                          cccACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                           <  2:155364/139‑1 (MQ=255)
                                            cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGCTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGtt                                                                         >  2:457656/1‑139 (MQ=255)
                                               tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAg                                                                      >  1:7404/1‑139 (MQ=255)
                                                   aCGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAg                                                                  <  2:1134953/139‑1 (MQ=255)
                                                    cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAg                                                                      <  2:640946/134‑1 (MQ=255)
                                                    cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAg                                                                      >  1:640946/1‑134 (MQ=255)
                                                      tCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTa                                                               >  1:276954/1‑139 (MQ=255)
                                                         ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTgggggg                                                                                                                      >  1:617812/1‑81 (MQ=255)
                                                         ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTgggggg                                                                                                                      <  2:617812/81‑1 (MQ=255)
                                                         ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATtgctgc                                                                                                    <  2:539855/99‑1 (MQ=255)
                                                         ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATtgctgc                                                                                                    >  1:539855/1‑99 (MQ=255)
                                                           tGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCtt                                                          >  1:338153/1‑139 (MQ=255)
                                                                           aCCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                          <  2:475125/139‑1 (MQ=255)
                                                                            ccGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTg                                         <  2:816333/139‑1 (MQ=255)
                                                                                       tgCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                           <  2:1220613/94‑1 (MQ=255)
                                                                                       tgCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                           >  1:1220613/1‑94 (MQ=255)
                                                                                            tCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                >  1:330862/1‑84 (MQ=255)
                                                                                            tCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                <  2:330862/84‑1 (MQ=255)
                                                                                              aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGc                                                                            >  1:51981/1‑86 (MQ=255)
                                                                                              aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGc                                                                            <  2:51981/86‑1 (MQ=255)
                                                                                                 ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCT‑GGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                           >  2:528876/1‑83 (MQ=255)
                                                                                                 ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCT‑GGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                           <  1:528876/83‑1 (MQ=255)
                                                                                                      ggcggcAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGcc               >  1:542666/1‑139 (MQ=255)
                                                                                                       gcggcAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCg              >  1:1110589/1‑139 (MQ=255)
                                                                                                                 caggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg    <  2:276954/139‑1 (MQ=255)
                                                                                                                   ggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                                                                 >  1:62338/1‑76 (MQ=255)
                                                                                                                   ggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                                                                 <  2:62338/76‑1 (MQ=255)
                                                                                                                   ggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcgcg  >  1:379896/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                            
CCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCG  >  NC_000913/1458488‑1458741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑