Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,605,180 A→G S44G (AGC→GGC)  lsrB → autoinducer 2‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,605,1800AG100.0% 95.7 / NA 28S44G (AGC→GGC) lsrBautoinducer 2‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

TTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAG  >  NC_000913/1605058‑1605300
                                                                                                                          |                                                                                                                        
ttCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGgcgc                                                                                                          >  1:167105/1‑139 (MQ=255)
    tCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAAGGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGTCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaa                                                                                                      <  2:392470/139‑1 (MQ=255)
     cGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaac                                                                                                     >  1:602736/1‑139 (MQ=255)
       cTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGGGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCAcaacaa                                                                                                   <  1:208499/139‑1 (MQ=255)
                               cgcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAt                                                                           >  1:1159050/1‑139 (MQ=255)
                                            gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGTGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                                                                          >  1:769478/1‑127 (MQ=255)
                                            gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                                                                          <  2:769478/127‑1 (MQ=255)
                                                               tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGgcg                                                                                                           >  1:621514/1‑75 (MQ=255)
                                                               tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGgcg                                                                                                           <  2:621514/75‑1 (MQ=255)
                                                                 cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGa                                                        <  2:637253/124‑1 (MQ=255)
                                                                 cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGa                                                        >  1:637253/1‑124 (MQ=255)
                                                                 cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGttt                                         >  2:1244364/1‑139 (MQ=255)
                                                                       gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTc                                   <  2:1159050/139‑1 (MQ=255)
                                                                       gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTc                                   <  2:167105/139‑1 (MQ=255)
                                                                          gggCGTATTACATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCCACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAgg                                <  2:763388/137‑1 (MQ=255)
                                                                             cGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTAc                             <  2:602736/139‑1 (MQ=255)
                                                                                    cATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTacga                                                                <  1:837127/97‑1 (MQ=255)
                                                                                    cATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTacga                                                                >  2:837127/1‑97 (MQ=255)
                                                                                       ttATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtgt                                                                         <  2:204606/85‑1 (MQ=255)
                                                                                       ttATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtgt                                                                         >  1:204606/1‑85 (MQ=255)
                                                                                       ttATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATtaa                   >  1:1059346/1‑139 (MQ=255)
                                                                                         aTTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaa                                                                                                      <  2:246907/54‑1 (MQ=255)
                                                                                         aTTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaa                                                                                                      >  1:246907/1‑54 (MQ=255)
                                                                                         aTTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATtaata                 >  1:744894/1‑139 (MQ=255)
                                                                                              cAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAg                                              >  1:750981/1‑105 (MQ=255)
                                                                                              cAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCCACAGAATCCAg                                              <  2:750981/105‑1 (MQ=255)
                                                                                                      ttGGCGTGGAATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGtcaatca    <  2:869442/139‑1 (MQ=255)
                                                                                                        ggCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAg  <  2:1059346/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                        
TTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAG  >  NC_000913/1605058‑1605300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: