Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,614,789 C→T intergenic (‑86/‑15) sad ← / → yneJ succinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,614,7890CT100.0% 97.8 / NA 31intergenic (‑86/‑15)sad/yneJsuccinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/15);  total (16/15)

ATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCT  >  NC_000913/1614665‑1614919
                                                                                                                            |                                                                                                                                  
aTTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCt                                                                                                                      >  1:117114/1‑139 (MQ=255)
                tGAGTTGCCGGCGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                      <  1:841315/139‑1 (MQ=255)
                                  gTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgaga                                                                                                                           >  2:637620/1‑100 (MQ=255)
                                  gTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgaga                                                                                                                           <  1:637620/100‑1 (MQ=255)
                                     aTCGGGGTATCTCCTTTATGAGTCATGGTATGGAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          >  2:768293/1‑98 (MQ=255)
                                     aTCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          <  1:768293/98‑1 (MQ=255)
                                        ggggTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCg                                                                              <  1:332997/139‑1 (MQ=255)
                                                            cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTa                                                                                                                     >  2:408772/1‑80 (MQ=255)
                                                            cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTa                                                                                                                     <  1:408772/80‑1 (MQ=255)
                                                            cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGa                                                                                                            <  1:280985/89‑1 (MQ=255)
                                                            cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGa                                                                                                            >  2:280985/1‑89 (MQ=255)
                                                             aTGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          <  1:451058/74‑1 (MQ=255)
                                                             aTGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          >  2:451058/1‑74 (MQ=255)
                                                               ggTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGAt                                                                                                                <  1:1230736/82‑1 (MQ=255)
                                                               ggTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGAt                                                                                                                >  2:1230736/1‑82 (MQ=255)
                                                               ggTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCgg                                                                                    >  1:1153905/1‑110 (MQ=255)
                                                               ggTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCgg                                                                                    <  2:1153905/110‑1 (MQ=255)
                                                                          aCGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCga                                                                                                                             >  1:387962/1‑58 (MQ=255)
                                                                          aCGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCga                                                                                                                             <  2:387962/58‑1 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          <  2:1209269/60‑1 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          >  1:1209269/1‑60 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGa                                                                                                            <  2:590964/74‑1 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGa                                                                                                            >  1:590964/1‑74 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                      >  2:1167783/1‑80 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAAc                                                                                                      <  1:1167783/80‑1 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                           >  1:173356/1‑139 (MQ=255)
                                                                           cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                           >  2:1286114/1‑139 (MQ=255)
                                                                                  ttACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATcgcg                                    <  2:173356/139‑1 (MQ=255)
                                                                                                                    cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTAc                 <  2:856794/124‑1 (MQ=255)
                                                                                                                    cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTAc                 >  1:856794/1‑124 (MQ=255)
                                                                                                                    cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt  >  2:315619/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                  
ATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCT  >  NC_000913/1614665‑1614919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: