Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,621,542 G→A D71N (GAC→AAC)  ydeE → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,621,5420GA100.0% 78.2 / NA 26D71N (GAC→AAC) ydeEputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/16);  total (10/16)

CTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTA  >  NC_000913/1621410‑1621674
                                                                                                                                    |                                                                                                                                    
cTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                                <  1:422424/139‑1 (MQ=255)
cTGACATTTATGACCATTTACTTTAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                                <  1:292939/139‑1 (MQ=255)
                tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                            >  2:706689/1‑127 (MQ=255)
                tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                            <  1:706689/127‑1 (MQ=255)
                  tACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTgg                                                                                                              <  1:786293/139‑1 (MQ=255)
                    cTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                            <  1:789991/123‑1 (MQ=255)
                    cTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                            >  2:789991/1‑123 (MQ=255)
                        aGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGaaa                                                                                                                              >  1:628038/1‑117 (MQ=255)
                        aGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGaaa                                                                                                                              <  2:628038/117‑1 (MQ=255)
                                                 aTCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGc                                                                               <  2:156998/139‑1 (MQ=255)
                                                           ttATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTa                                                                     <  1:1220605/139‑1 (MQ=255)
                                                                     aCAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                            >  2:1235083/1‑74 (MQ=255)
                                                                     aCAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                            <  1:1235083/74‑1 (MQ=255)
                                                                     aCAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCg                                                                                         >  1:751284/1‑109 (MQ=255)
                                                                     aCAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCg                                                                                         <  2:751284/109‑1 (MQ=255)
                                                                                   tATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTtgtg                                             <  2:7193/139‑1 (MQ=255)
                                                                                           tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCttttt                                     >  2:143449/1‑139 (MQ=255)
                                                                                                  tAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          <  2:474998/95‑1 (MQ=255)
                                                                                                  tAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                          >  1:474998/1‑95 (MQ=255)
                                                                                                                     cTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACttt                                                                      <  2:246043/80‑1 (MQ=255)
                                                                                                                     cTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACttt                                                                      >  1:246043/1‑80 (MQ=255)
                                                                                                                        gCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtt                                                                                      >  1:1184593/1‑61 (MQ=255)
                                                                                                                        gCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGtt                                                                                      <  2:1184593/61‑1 (MQ=255)
                                                                                                                              aaGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAAc                                                            <  1:784804/81‑1 (MQ=255)
                                                                                                                              aaGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAAc                                                            >  2:784804/1‑81 (MQ=255)
                                                                                                                              aaGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTa  <  1:143449/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
CTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTA  >  NC_000913/1621410‑1621674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: