Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,627,625 T→C L37L (TTG→CTG)  ydfG → NADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,627,6250TC100.0% 208.2 / NA 57L37L (TTG→CTG) ydfGNADP‑dependent 3‑hydroxy acid dehydrogenase, malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

ACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATG  >  NC_000913/1627493‑1627760
                                                                                                                                    |                                                                                                                                       
acaAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAgg                                                                                                                                   >  1:443970/1‑139 (MQ=255)
    aaGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGtt                                                                                                                               <  2:443970/139‑1 (MQ=255)
          tGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGa                                                                                                                         >  1:1043887/1‑139 (MQ=255)
            aGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg                                                                                                                       <  1:367619/139‑1 (MQ=255)
            aGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACg                                                                                                                       <  2:1043887/139‑1 (MQ=255)
                aCAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACt                                                                                                                   <  2:470569/139‑1 (MQ=255)
                    tGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGga                                                                                                               >  2:847536/1‑139 (MQ=255)
                       gatgatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                                            <  1:115436/139‑1 (MQ=255)
                          gatCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCCTAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAAt                                                                                                         <  1:808543/139‑1 (MQ=255)
                           atCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTg                                                                                                                                       >  1:420694/1‑108 (MQ=255)
                              gTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                                            >  1:811223/1‑132 (MQ=255)
                              gTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGAt                                                                                                            <  2:811223/132‑1 (MQ=255)
                                 ttAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGtata                                                                                                  >  2:1000021/1‑139 (MQ=255)
                                       aCTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa                                                                                                                                     >  2:1235171/1‑98 (MQ=255)
                                       aCTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCa                                                                                                                                     <  1:1235171/98‑1 (MQ=255)
                                         tGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCaa                                                                                          <  1:1000021/139‑1 (MQ=255)
                                          ggAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAAc                                                                                         >  1:1269421/1‑139 (MQ=255)
                                                       gTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgaga                                                                                                             <  1:1294323/106‑1 (MQ=255)
                                                       gTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgaga                                                                                                             >  2:1294323/1‑106 (MQ=255)
                                                           tGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACcgc                                                                        >  1:1173638/1‑139 (MQ=255)
                                                             gTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc                                                                      <  1:706136/139‑1 (MQ=255)
                                                               gAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg                                                                    <  2:1269421/139‑1 (MQ=255)
                                                                 aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              >  1:640853/1‑95 (MQ=255)
                                                                 aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  2:640853/95‑1 (MQ=255)
                                                                 aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg                                                                    <  1:308435/137‑1 (MQ=255)
                                                                 aTGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGcgccg                                                                    >  2:308435/1‑137 (MQ=255)
                                                                     aTTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTg                                                              >  1:1051530/1‑139 (MQ=255)
                                                                              cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGtt                                                                                                                               <  1:425356/65‑1 (MQ=255)
                                                                              cgtTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGtt                                                                                                                               >  2:425356/1‑65 (MQ=255)
                                                                                   tATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCa                                                >  1:293346/1‑139 (MQ=255)
                                                                                        aacaaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCt                                           >  2:1189075/1‑139 (MQ=255)
                                                                                          caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                                                    >  2:519175/1‑48 (MQ=255)
                                                                                          caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAg                                                                                                                                    <  1:519175/48‑1 (MQ=255)
                                                                                          caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                                      >  2:1040582/1‑94 (MQ=255)
                                                                                          caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                                      <  1:1040582/94‑1 (MQ=255)
                                                                                          caaGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTc                                         <  1:847536/139‑1 (MQ=255)
                                                                                             gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              >  1:386514/1‑67 (MQ=255)
                                                                                             gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGgag                                                                                                              <  2:386514/67‑1 (MQ=255)
                                                                                             gggCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTg                                      <  2:293346/139‑1 (MQ=255)
                                                                                               gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCg                                                                               >  2:996323/1‑96 (MQ=255)
                                                                                               gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCg                                                                               <  1:996323/96‑1 (MQ=255)
                                                                                               gCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGcc                                    >  1:129914/1‑139 (MQ=255)
                                                                                                       ttATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAgtggtg                            <  2:1173638/139‑1 (MQ=255)
                                                                                                        tATCGCCACTGGCCGTCGCCAGGCACGGCTGCAGGAGTTATAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                                      <  2:855850/80‑1 (MQ=255)
                                                                                                        tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTgg                                                                                      >  1:855850/1‑80 (MQ=255)
                                                                                                        tATCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTAGTGAAGAGATGGTGGCATCGCTTCCTGCCGAGTGGTGc                           >  2:824105/1‑139 (MQ=255)
                                                                                                         aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGTGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCa                          <  2:1051530/139‑1 (MQ=255)
                                                                                                         aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGTGAGATAATCTGTATATCGCCCAATTGGACGTTCGCAACCACGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCa                          <  1:824105/139‑1 (MQ=255)
                                                                                                         aTCGCCACTGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCa                          <  2:149730/139‑1 (MQ=255)
                                                                                                               aCTGACCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCaa                                                                            >  1:1096914/1‑83 (MQ=255)
                                                                                                               aCTGACCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCaa                                                                            <  2:1096914/83‑1 (MQ=255)
                                                                                                                 tGGCCGTCGCCAGGAACGGCTGCAGGAGTTCAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc                                                                      <  2:857702/87‑1 (MQ=255)
                                                                                                                 tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCgcgc                                                                      >  1:857702/1‑87 (MQ=255)
                                                                                                                 tGGCCGTCGCCAGGAACGGCTGCAGGAGTTAAAAGACGAACTGGAAGATAATGTGTATATCGCCCAACTGGCCGTTCGCAACCGGGCCGCTATTGAAAGGATGATGGCACCCCTTCCTGCTGAGTGGGCCACTATTgtt                  >  2:240578/1‑137 (MQ=255)
                                                                                                                             ggAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGtata                                                                                                  <  1:928389/47‑1 (MQ=255)
                                                                                                                             ggAACGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGtata                                                                                                  >  2:928389/1‑47 (MQ=255)
                                                                                                                                 cGGCTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATg  <  1:1189075/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
ACAAAAGCGTTGAGGAACAGTGAGATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGTTATCGCCACTGGCCGTCGCCAGGAACGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGGACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAATAATG  >  NC_000913/1627493‑1627760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑