Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,484	T→C	V36A (GTC→GCC)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,484	0	T	C	100.0%	119.0 / NA	34	V36A (GTC→GCC)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

ATGCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAA  >  NC_000913/1735349‑1735613                                                                                                                                        |                                                                                                                                  aTGCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCAc                                                                                                                                >  1:747231/1‑139 (MQ=255)                       agTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTaaa                                                                                                          >  1:778687/1‑139 (MQ=255)                         tAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCa                                                                                                                                 >  1:980613/1‑114 (MQ=255)                                 tCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   <  1:1010118/120‑1 (MQ=255)                                 tCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   >  2:1010118/1‑120 (MQ=255)                                   aTTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGttt                                                                                              >  2:197392/1‑139 (MQ=255)                                     tCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   >  2:51162/1‑116 (MQ=255)                                     tCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   <  1:51162/116‑1 (MQ=255)                                                      ccATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAag                                                                           >  1:973966/1‑139 (MQ=255)                                                         tatGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAgaga                                                                        <  1:197392/139‑1 (MQ=255)                                                           tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGatt                                                                      >  1:441487/1‑139 (MQ=255)                                                              tAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGattatt                                                                   <  2:973966/139‑1 (MQ=255)                                                                aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGt                                                                                                       >  1:1075188/1‑101 (MQ=255)                                                                aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGt                                                                                                       <  2:1075188/101‑1 (MQ=255)                                                                aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                 <  2:778687/139‑1 (MQ=255)                                                                     gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGct                                                            >  2:1081914/1‑139 (MQ=255)                                                                     gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGct                                                            >  2:1219347/1‑139 (MQ=255)                                                                       tctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   >  1:709076/1‑82 (MQ=255)                                                                       tctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   <  2:709076/82‑1 (MQ=255)                                                                       tctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   >  1:204369/1‑82 (MQ=255)                                                                       tctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   <  2:204369/82‑1 (MQ=255)                                                                               cGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   <  1:1257030/74‑1 (MQ=255)                                                                               cGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   >  2:1257030/1‑74 (MQ=255)                                                                                gCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   <  2:1185364/73‑1 (MQ=255)                                                                                gCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                   >  1:1185364/1‑73 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                              <  2:856357/126‑1 (MQ=255)                                                                                                aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                              >  1:856357/1‑126 (MQ=255)                                                                                                  cATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCt                               <  1:1081914/139‑1 (MQ=255)                                                                                                                      aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACACAGCTCAGGTCTGGAACCACACttt           >  1:803918/1‑139 (MQ=255)                                                                                                                       gCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTc          >  1:285266/1‑139 (MQ=255)                                                                                                                          ccATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTAc       <  2:441487/139‑1 (MQ=255)                                                                                                                               aGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATGATTCGGAGCGCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCCGGAACCATACTTTCTACTGGaa  <  2:747231/139‑1 (MQ=255)                                                                                                                               aGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGACATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGaa  <  2:285266/139‑1 (MQ=255)                                                                                                                                     aTGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCa                                                                                  <  2:13297/53‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  ATGCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAA  >  NC_000913/1735349‑1735613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑