Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,799,883	T→C	K39K (AAA→AAG)	rpmI ←	50S ribosomal subunit protein L35

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,799,883	0	T	C	100.0%	89.6 / NA	26	K39K (AAA→AAG)	rpmI	50S ribosomal subunit protein L35
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/11);  total (15/11)

ATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGC  >  NC_000913/1799768‑1799997                                                                                                                    |                                                                                                                   atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAt                                                                                             >  2:835822/1‑139 (MQ=255)   atTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAAtgt                                                                                           >  2:404286/1‑139 (MQ=255)      cTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGAc                                                                                        >  2:1056724/1‑139 (MQ=255)                            tAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgc                                                                          >  2:836078/1‑131 (MQ=255)                            tAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgc                                                                          <  1:836078/131‑1 (MQ=255)                                    tATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGt                                                                                  <  1:656379/115‑1 (MQ=255)                                    tATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGt                                                                                  >  2:656379/1‑115 (MQ=255)                                           aCGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTAcc                                                   >  1:756034/1‑139 (MQ=255)                                           aCGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTAcc                                                   >  1:205285/1‑139 (MQ=255)                                                aggcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgtttacgctt                                                                                                                  >  1:1245682/1‑71 (MQ=255)                                                aggcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGacgtttacgctt                                                                                                                  <  2:1245682/71‑1 (MQ=255)                                                aggcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGttt                                              >  2:1111471/1‑139 (MQ=255)                                                        gATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAg                                      <  1:1111471/139‑1 (MQ=255)                                                          ttACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                    <  1:97404/139‑1 (MQ=255)                                                          ttACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                    <  2:205285/139‑1 (MQ=255)                                                                  cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCtttt                                                                                                        <  2:31308/63‑1 (MQ=255)                                                                  cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCtttt                                                                                                        >  1:31308/1‑63 (MQ=255)                                                                  cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                    <  2:1188494/131‑1 (MQ=255)                                                                  cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                    >  1:1188494/1‑131 (MQ=255)                                                                  cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                    >  1:774084/1‑131 (MQ=255)                                                                  cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTagcag                            <  1:989505/139‑1 (MQ=255)                                                                  cccAGATCGCCTTTGGAAACCATGGCTTTCGGAAGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                    <  2:774084/131‑1 (MQ=255)                                                                      gATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCAcc                        >  2:743241/1‑139 (MQ=255)                                                                           ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACg                                                                                       <  2:44346/71‑1 (MQ=255)                                                                           ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACg                                                                                       >  1:44346/1‑71 (MQ=255)                                                                               tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGt               >  2:177678/1‑139 (MQ=255)                                                                               tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGt               >  1:97916/1‑139 (MQ=255)                                                                                            tttCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGc  <  1:177678/139‑1 (MQ=255)                                                                                                                    |                                                                                                                   ATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGC  >  NC_000913/1799768‑1799997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑