Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,810	G→A	*322* (TAG→TAA)	yeaX →	putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,810	0	G	A	100.0%	90.4 / NA	29	*322* (TAG→TAA)	yeaX	putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

CGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAG  >  NC_000913/1886678‑1886935                                                                                                                                     |                                                                                                                              cGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                         >  1:1078374/1‑139 (MQ=255)     aCAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAg                                                                                                                     <  2:1078374/139‑1 (MQ=255)                                cATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                          >  2:1210262/1‑139 (MQ=255)                                    gggATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCgg                                                                                      >  2:1278621/1‑139 (MQ=255)                                                      aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                 >  1:304624/1‑126 (MQ=255)                                                      aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                 <  2:304624/126‑1 (MQ=255)                                                      aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTg                                                                    >  2:533567/1‑139 (MQ=255)                                                         aaGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgc                                                                                                   >  1:1277757/1‑105 (MQ=255)                                                         aaGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgc                                                                                                   <  2:1277757/105‑1 (MQ=255)                                                                 gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCt                                                         >  1:920111/1‑139 (MQ=255)                                                                      ccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAAt                                                    <  2:920111/139‑1 (MQ=255)                                                                                       tCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                         <  1:916484/53‑1 (MQ=255)                                                                                       tCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGGAATTTGcc                                                                                                                         >  2:916484/1‑53 (MQ=255)                                                                                           ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                         >  1:1158753/1‑49 (MQ=255)                                                                                           ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                         <  2:1158753/49‑1 (MQ=255)                                                                                           ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                 <  2:1220044/57‑1 (MQ=255)                                                                                           ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                 >  1:1220044/1‑57 (MQ=255)                                                                                           ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtg                                                                                                              <  1:814249/60‑1 (MQ=255)                                                                                           ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtg                                                                                                              >  2:814249/1‑60 (MQ=255)                                                                                                cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                      >  1:43457/1‑95 (MQ=255)                                                                                                cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                      <  2:43457/95‑1 (MQ=255)                                                                                                cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg                          >  1:314842/1‑139 (MQ=255)                                                                                                      cGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                 >  2:1289456/1‑46 (MQ=255)                                                                                                      cGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                 <  1:1289456/46‑1 (MQ=255)                                                                                                               aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGcc           >  1:936298/1‑139 (MQ=255)                                                                                                                aCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCg          <  2:314842/139‑1 (MQ=255)                                                                                                                     tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg                                                                                                <  1:899170/48‑1 (MQ=255)                                                                                                                     tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg                                                                                                >  2:899170/1‑48 (MQ=255)                                                                                                                        tgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAg  <  2:936298/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                              CGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAG  >  NC_000913/1886678‑1886935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑