Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,993	G→A	G111E (GGG→GAG)	znuC →	zinc ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,993	0	G	A	100.0%	180.6 / NA	56	G111E (GGG→GAG)	znuC	zinc ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/29);  total (27/29)

AAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTA  >  NC_000913/1942857‑1943120                                                                                                                                         |                                                                                                                                aaaCTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGc                                                                                                                               <  1:838871/139‑1 (MQ=255)             ggCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACg                                                                                                                   <  2:1159426/139‑1 (MQ=255)             ggCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACg                                                                                                                   <  2:514792/139‑1 (MQ=255)              gCTATGTACCGCAGAAGCTGTATCTCAACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGc                                                                                                                  >  2:1056553/1‑139 (MQ=255)                        gCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaa                                                                                                        <  2:176126/139‑1 (MQ=255)                         cAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaaa                                                                                                       >  2:192957/1‑139 (MQ=255)                            aaGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTACACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGc                                                                                                    >  2:585591/1‑139 (MQ=255)                              gCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGctc                                                                                                  >  1:677533/1‑139 (MQ=255)                                            ccacGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAAc                                                                                    >  1:281139/1‑139 (MQ=255)                                             cacGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                   <  2:993813/139‑1 (MQ=255)                                              acGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc                                                                                  <  2:894379/139‑1 (MQ=255)                                               cGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAg                                                                                                     <  1:205133/119‑1 (MQ=255)                                               cGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAg                                                                                                     >  2:205133/1‑119 (MQ=255)                                               cGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                 >  2:377978/1‑139 (MQ=255)                                                gTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                <  2:281139/139‑1 (MQ=255)                                                gTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                <  2:279148/139‑1 (MQ=255)                                                gTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                <  1:192957/139‑1 (MQ=255)                                                                 aCCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTa                                                                                                                      >  2:291776/1‑84 (MQ=255)                                                                 aCCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTa                                                                                                                      <  1:291776/84‑1 (MQ=255)                                                                      tttttACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTaa                                                                                                                     >  2:1039212/1‑80 (MQ=255)                                                                      tttttACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTaa                                                                                                                     <  1:1039212/80‑1 (MQ=255)                                                                                       tGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCaa                                         >  1:130132/1‑139 (MQ=255)                                                                                           acacATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAattat                                     <  1:377978/139‑1 (MQ=255)                                                                                               aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGGAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGc                                               <  1:789469/125‑1 (MQ=255)                                                                                               aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGc                                               >  2:789469/1‑125 (MQ=255)                                                                                                tAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTg                                <  2:677533/139‑1 (MQ=255)                                                                                                   agaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGcc                                              <  2:24216/122‑1 (MQ=255)                                                                                                   agaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGcc                                              >  1:24216/1‑122 (MQ=255)                                                                                                       gaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGGTGCAAAAg                                                                                                     <  1:841345/63‑1 (MQ=255)                                                                                                       gaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAg                                                                                                     >  2:841345/1‑63 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATg                                                                                                           <  2:1053195/55‑1 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATg                                                                                                           >  1:1053195/1‑55 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTATCGCGAGCATTGTTAAATCGccc                                             <  2:933698/117‑1 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGccc                                             >  1:933698/1‑117 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGa                           >  2:450182/1‑135 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGa                           <  1:450182/135‑1 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGaa                       >  1:1011020/1‑139 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGaa                       <  2:1011020/139‑1 (MQ=255)                                                                                                         tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGACTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGaa                       >  1:834065/1‑139 (MQ=255)                                                                                                             ttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCa                   >  1:110138/1‑139 (MQ=255)                                                                                                               gCCTGCACTGAAACGTGTCCAGGCCGAGCGCCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGa                           <  2:1139153/129‑1 (MQ=255)                                                                                                               gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGCGGCGAAACGCAGCGTGTAGTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGa                           >  1:1139153/1‑129 (MQ=255)                                                                                                                 cTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                >  2:1276847/1‑74 (MQ=255)                                                                                                                 cTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                <  1:1276847/74‑1 (MQ=255)                                                                                                                     aCTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGc           <  2:983424/139‑1 (MQ=255)                                                                                                                              tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                 >  2:347504/1‑60 (MQ=255)                                                                                                                              tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                 <  1:347504/60‑1 (MQ=255)                                                                                                                              tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                 >  2:1191017/1‑60 (MQ=255)                                                                                                                              tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                 <  1:1191017/60‑1 (MQ=255)                                                                                                                              tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                             <  2:1082208/80‑1 (MQ=255)                                                                                                                              tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                             >  1:1082208/1‑80 (MQ=255)                                                                                                                              tgtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGTCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTa  >  2:60257/1‑139 (MQ=255)                                                                                                                                tCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTACATCGCCCGCAATTATTAGTg                                <  1:980502/107‑1 (MQ=255)                                                                                                                                tCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTtgtg                                >  2:980502/1‑107 (MQ=255)                                                                                                                                 ccAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg                                                                            <  2:10257/62‑1 (MQ=255)                                                                                                                                 ccAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg                                                                            >  1:10257/1‑62 (MQ=255)                                                                                                                                         |                                                                                                                                AAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTA  >  NC_000913/1942857‑1943120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑