Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,399	C→T	P488S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,399	0	C	T	100.0%	89.7 / NA	29	P488S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/13);  total (16/13)

GAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAG  >  NC_000913/2046275‑2046533                                                                                                                             |                                                                                                                                       gAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTc                                                                                                                          <  1:1272582/139‑1 (MQ=255)       aCTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTAc                                                                                                                                <  1:1066854/127‑1 (MQ=255)       aCTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTAc                                                                                                                                >  2:1066854/1‑127 (MQ=255)         tGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTAc                                                                                                                  >  1:867535/1‑139 (MQ=255)                                    cccTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCtt                                                                                                                                  <  1:1098831/96‑1 (MQ=255)                                    cccTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCtt                                                                                                                                  >  2:1098831/1‑96 (MQ=255)                                    cccTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGAt                                                                                       >  1:156825/1‑139 (MQ=255)                                              cTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGt                                                                                 >  1:1285950/1‑135 (MQ=255)                                              cTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGt                                                                                 <  2:1285950/135‑1 (MQ=255)                                                 taACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCAccgcc                                                                          >  2:740582/1‑139 (MQ=255)                                                                cGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGc                                                           >  1:1034819/1‑139 (MQ=255)                                                                        aaGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGGTTGAAGTCACCGCCGAAGATGTCGAACGCAATTTGTc                                                   <  2:867535/139‑1 (MQ=255)                                                                        aaGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTc                                                   <  2:156825/139‑1 (MQ=255)                                                                             gCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATc                                              >  1:71745/1‑139 (MQ=255)                                                                              ccGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCg                                             <  1:740582/139‑1 (MQ=255)                                                                                  tGGCAAAGTGGTCACACCGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGaa                                         <  1:626104/139‑1 (MQ=255)                                                                                    gCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACa                                       <  2:71745/139‑1 (MQ=255)                                                                                              cacaACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGaa                                         >  1:1149931/1‑127 (MQ=255)                                                                                              cacaACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGaa                                         <  2:1149931/127‑1 (MQ=255)                                                                                                      gTAAAGATATTCTGGTTACCCTGTAGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCa                     <  1:720748/139‑1 (MQ=255)                                                                                                            atatTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc               <  1:906174/139‑1 (MQ=255)                                                                                                               ttCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTAc            >  1:949455/1‑139 (MQ=255)                                                                                                                tCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACg           >  2:18986/1‑139 (MQ=255)                                                                                                                    gTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           >  1:568840/1‑55 (MQ=255)                                                                                                                    gTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           <  2:568840/55‑1 (MQ=255)                                                                                                                      tACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGc     >  2:1241440/1‑139 (MQ=255)                                                                                                                         ccTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAg  >  2:1212084/1‑139 (MQ=255)                                                                                                                         ccTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAg  >  2:1149948/1‑139 (MQ=255)                                                                                                                         ccTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAg  >  1:73688/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                       GAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAG  >  NC_000913/2046275‑2046533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑