Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,139,759	C→T	*618* (TAG→TAA)	asmA ←	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,139,759	0	C	T	100.0%	197.8 / NA	62	*618* (TAG→TAA)	asmA	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/30);  total (32/30)

CGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGG  >  NC_000913/2139632‑2139888                                                                                                                                |                                                                                                                                  cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                        >  2:629696/1‑139 (MQ=255) cGAGGAACACCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                        >  1:180506/1‑139 (MQ=255)   aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                               >  2:708591/1‑130 (MQ=255)   aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                               <  1:708591/130‑1 (MQ=255)   aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                                      >  2:796498/1‑139 (MQ=255)   aGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCa                                                                                                                      >  1:883085/1‑139 (MQ=255)     gAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGc                                                                                                                    >  2:487386/1‑139 (MQ=255)        cATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                 <  1:487386/139‑1 (MQ=255)        cATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCATATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                 >  1:1194077/1‑139 (MQ=255)             aGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAActtctt                                                                                                            <  2:180506/139‑1 (MQ=255)                      aaCAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                   >  2:1018905/1‑139 (MQ=255)                       aCAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCACCAGCAACTTCTTCACATCTTTg                                                                                                  <  2:1194077/139‑1 (MQ=255)                             ttCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                            <  1:1018905/139‑1 (MQ=255)                                 aTCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTCATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        <  1:778821/139‑1 (MQ=255)                                    ttACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAAt                                                                                     <  1:629696/139‑1 (MQ=255)                                            tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        <  2:536156/128‑1 (MQ=255)                                            tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        >  1:536156/1‑128 (MQ=255)                                                aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                         >  1:140417/1‑139 (MQ=255)                                                aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                         >  1:739387/1‑139 (MQ=255)                                                     aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                         >  1:273070/1‑134 (MQ=255)                                                     aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                         <  2:273070/134‑1 (MQ=255)                                                          tgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTc                                                               >  1:1097332/1‑139 (MQ=255)                                                          tgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTc                                                               >  2:1272739/1‑139 (MQ=255)                                                            ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                 <  2:1162707/87‑1 (MQ=255)                                                            ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                 >  1:1162707/1‑87 (MQ=255)                                                              gccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                         <  1:748965/125‑1 (MQ=255)                                                              gccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTAGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                         >  2:748965/1‑125 (MQ=255)                                                                 aCGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAgg                                                        <  1:796498/139‑1 (MQ=255)                                                                             tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                            <  1:1272739/139‑1 (MQ=255)                                                                             tGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                            <  2:597730/139‑1 (MQ=255)                                                                                gACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                                         <  1:339163/139‑1 (MQ=255)                                                                                 aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACg                                                                                          <  2:540419/89‑1 (MQ=255)                                                                                 aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACg                                                                                          >  1:540419/1‑89 (MQ=255)                                                                                 aCGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGt                                        >  2:481757/1‑139 (MQ=255)                                                                                  cGCTTTAGGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCCACTTCTTCACATCTTTGCCATTACGGGAATCTTTCTTCCGCTCGCCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                                       >  2:1287563/1‑139 (MQ=255)                                                                                  cGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                                       <  1:481757/139‑1 (MQ=255)                                                                                   gCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTcc                                      >  1:969087/1‑139 (MQ=255)                                                                                               ttATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGc                                                                                                                    >  2:648299/1‑49 (MQ=255)                                                                                               ttATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGc                                                                                                                    <  1:648299/49‑1 (MQ=255)                                                                                                 aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCAGCTCGGCACAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgag                        >  2:678054/1‑137 (MQ=255)                                                                                                    aGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAg                     <  2:313223/139‑1 (MQ=255)                                                                                                     ggCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCAGTAGATGTTTGCGCAGt                    <  2:739387/139‑1 (MQ=255)                                                                                                      gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGa                                                                                       <  2:1267421/71‑1 (MQ=255)                                                                                                      gCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGa                                                                                       >  1:1267421/1‑71 (MQ=255)                                                                                                         tACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACt                <  2:1097332/139‑1 (MQ=255)                                                                                                           cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        <  1:515069/65‑1 (MQ=255)                                                                                                           cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        >  2:515069/1‑65 (MQ=255)                                                                                                           cAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGa              <  1:850374/139‑1 (MQ=255)                                                                                                                cTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                 <  1:554399/35‑1 (MQ=255)                                                                                                                cTGGTCACCTGGTAACTTACATCTTCTCCAGCAAc                                                                                                                 >  2:554399/1‑35 (MQ=255)                                                                                                                cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                            <  2:323043/104‑1 (MQ=255)                                                                                                                cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCt                                            >  1:323043/1‑104 (MQ=255)                                                                                                                cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                                         <  1:422945/107‑1 (MQ=255)                                                                                                                cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                                         >  2:422945/1‑107 (MQ=255)                                                                                                                 tGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACt        >  1:882009/1‑139 (MQ=255)                                                                                                                 tGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACt        >  1:1014909/1‑139 (MQ=255)                                                                                                                   gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGc                                                                                                 <  2:225887/48‑1 (MQ=255)                                                                                                                   gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGc                                                                                                 >  1:225887/1‑48 (MQ=255)                                                                                                                   gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTg      >  1:1208023/1‑139 (MQ=255)                                                                                                                       ccTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAgg  <  1:678054/139‑1 (MQ=255)                                                                                                                        cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                        <  2:4744/116‑1 (MQ=255)                                                                                                                        cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                        >  1:4744/1‑116 (MQ=255)                                                                                                                                |                                                                                                                                  CGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGG  >  NC_000913/2139632‑2139888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑