Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,182,061	G→A	*322* (TAG→TAA)  L241L (CTA→TTA)	yegV → yegW ←	putative kinase putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,182,061	0	G	A	100.0%	116.7 / NA	37	*322* (TAG→TAA)  L241L (CTA→TTA)	yegV yegW	putative kinase putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/19);  total (18/19)

GCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGC  >  NC_000913/2181933‑2182188                                                                                                                                 |                                                                                                                                gCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                                                       >  1:379327/1‑139 (MQ=255)      tGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGt                                                                                                                   <  1:681333/138‑1 (MQ=255)      tGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGt                                                                                                                   >  2:681333/1‑138 (MQ=255)        gTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                                                <  2:721089/139‑1 (MQ=255)             ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATg                                                                                                           >  1:656578/1‑139 (MQ=255)               aCTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCt                                                                                                         <  2:581224/139‑1 (MQ=255)                            gTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGc                                                                                            <  1:1116057/139‑1 (MQ=255)                             tATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCACTGCGACAGTGGc                                                                                                                <  2:300766/118‑1 (MQ=255)                             tATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCACTGCGACAGTGGc                                                                                                                >  1:300766/1‑118 (MQ=255)                                     ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGt                                                                                         >  1:551976/1‑133 (MQ=255)                                     ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGt                                                                                         <  2:551976/133‑1 (MQ=255)                                     ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGt                                                                                   >  1:342397/1‑139 (MQ=255)                                     ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGt                                                                                   >  2:1027368/1‑139 (MQ=255)                                         aTGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGt                                                                               >  2:1181897/1‑139 (MQ=255)                                          tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                                              >  1:84516/1‑139 (MQ=255)                                          tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                                              >  2:526800/1‑139 (MQ=255)                                            cAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCaa                                                                            <  1:417641/139‑1 (MQ=255)                                               tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                                                          >  1:549597/1‑122 (MQ=255)                                               tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                                                          <  2:549597/122‑1 (MQ=255)                                               tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGt                                                                               >  2:627867/1‑133 (MQ=255)                                               tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGt                                                                               <  1:627867/133‑1 (MQ=255)                                               tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcg                                                                         <  1:1229616/139‑1 (MQ=255)                                                     cgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                   <  1:1181897/139‑1 (MQ=255)                                                        gggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgct                                                                <  2:84516/139‑1 (MQ=255)                                                          gTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                                              <  2:342397/139‑1 (MQ=255)                                                               cGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc                                                         <  2:656578/139‑1 (MQ=255)                                                                gggCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAcc                                                        <  1:526800/139‑1 (MQ=255)                                                                        gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCAc                                                   <  2:273313/136‑1 (MQ=255)                                                                        gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCAc                                                   >  1:273313/1‑136 (MQ=255)                                                                                   gtgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTg                                                                                                                          <  1:571995/54‑1 (MQ=255)                                                                                   gtgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTg                                                                                                                          >  2:571995/1‑54 (MQ=255)                                                                                   gtgCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTAt                                     >  1:311261/1‑139 (MQ=255)                                                                                     gcgcCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                                   <  2:311261/139‑1 (MQ=255)                                                                                                               cGCACTCAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggc         >  1:68692/1‑139 (MQ=255)                                                                                                               cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTa                                                                                                      >  2:248267/1‑46 (MQ=255)                                                                                                               cGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTa                                                                                                      <  1:248267/46‑1 (MQ=255)                                                                                                                      aaaaaCGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccggc  >  2:413495/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                GCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGC  >  NC_000913/2181933‑2182188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑