Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,189,594 C→T A439A (GCG→GCA)  yehB ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,189,5940CT100.0% 129.1 / NA 42A439A (GCG→GCA) yehBputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/17);  total (25/17)

GTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAT  >  NC_000913/2189495‑2189725
                                                                                                   |                                                                                                                                   
gTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATttg                                                                                              >  2:990166/1‑139 (MQ=255)
         aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCa                                                                                     >  2:1212122/1‑139 (MQ=255)
           cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAAt                                                                                                 <  1:32118/125‑1 (MQ=255)
           cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAAt                                                                                                 >  2:32118/1‑125 (MQ=255)
           cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                   >  2:951081/1‑139 (MQ=255)
           cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                   >  2:931954/1‑139 (MQ=255)
           cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAt                                                                                   >  1:831620/1‑139 (MQ=255)
                                    tttATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAAcaca                                                          <  1:1212122/139‑1 (MQ=255)
                                         tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGcc                                                     >  2:626909/1‑139 (MQ=255)
                                            ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGc                                                                                      >  1:520792/1‑103 (MQ=255)
                                            ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGc                                                                                      <  2:520792/103‑1 (MQ=255)
                                            ttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  1:951081/139‑1 (MQ=255)
                                             tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACttt                                                                        >  2:312955/1‑116 (MQ=255)
                                             tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACttt                                                                        <  1:312955/116‑1 (MQ=255)
                                             tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTg                                                 >  1:621227/1‑139 (MQ=255)
                                              gCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                >  2:563450/1‑139 (MQ=255)
                                                         aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGAc                                                                                                                              <  2:523883/50‑1 (MQ=255)
                                                         aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGAc                                                                                                                              >  1:523883/1‑50 (MQ=255)
                                                         aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGtt                                                                                                             >  1:103470/1‑67 (MQ=255)
                                                         aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGtt                                                                                                             <  2:103470/67‑1 (MQ=255)
                                                         aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTa                                     >  1:1212559/1‑139 (MQ=255)
                                                         aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTa                                     >  2:682510/1‑139 (MQ=255)
                                                           cGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                   <  1:682510/139‑1 (MQ=255)
                                                              tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                    >  1:339671/1‑87 (MQ=255)
                                                              tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                    <  2:339671/87‑1 (MQ=255)
                                                              tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGa                                >  1:910328/1‑139 (MQ=255)
                                                                     ccGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                       <  2:611633/109‑1 (MQ=255)
                                                                     ccGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                       >  1:611633/1‑109 (MQ=255)
                                                                      cGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  2:718938/113‑1 (MQ=255)
                                                                      cGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  >  1:718938/1‑113 (MQ=255)
                                                                       ggTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                       <  2:75980/107‑1 (MQ=255)
                                                                       ggTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                       >  1:75980/1‑107 (MQ=255)
                                                                         tAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  >  2:390630/1‑110 (MQ=255)
                                                                         tAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                  <  1:390630/110‑1 (MQ=255)
                                                                              aCGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACg                >  2:452588/1‑139 (MQ=255)
                                                                                      aaTAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGc        >  1:853205/1‑139 (MQ=255)
                                                                                        tAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCAc      <  1:931954/139‑1 (MQ=255)
                                                                                            gCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAt  <  2:187321/139‑1 (MQ=255)
                                                                                              cAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                          >  1:619929/1‑97 (MQ=255)
                                                                                              cAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                          <  2:619929/97‑1 (MQ=255)
                                                                                                ggCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                          <  2:1116095/95‑1 (MQ=255)
                                                                                                ggCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                          >  1:1116095/1‑95 (MQ=255)
                                                                                                   |                                                                                                                                   
GTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAT  >  NC_000913/2189495‑2189725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑