Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,973	T→C	G146G (GGA→GGG)	ccmH ←	heme lyase, CcmH subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,973	0	T	C	100.0%	106.6 / NA	32	G146G (GGA→GGG)	ccmH	heme lyase, CcmH subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACT  >  NC_000913/2291846‑2292084                                                                                                                                |                                                                                                                ccAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCtt                                                                                                      <  2:977360/139‑1 (MQ=255)        cGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTcc                                                                                               >  2:180071/1‑139 (MQ=255)        cGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTcc                                                                                               >  1:43659/1‑139 (MQ=255)          ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCgg                                                                                             >  1:1271030/1‑139 (MQ=255)                         tGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAgg                                                                              >  1:1023576/1‑139 (MQ=255)                              tgctgcCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACg                                                                         >  1:115119/1‑139 (MQ=255)                                        ttttCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcac                                                               <  1:1090528/139‑1 (MQ=255)                                                              aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         <  2:115119/139‑1 (MQ=255)                                                              aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         <  2:1271030/139‑1 (MQ=255)                                                              aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         <  2:43659/139‑1 (MQ=255)                                                              aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         <  2:964718/139‑1 (MQ=255)                                                              aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         <  1:1130018/139‑1 (MQ=255)                                                               gTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGTACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGc                                        >  2:1235026/1‑139 (MQ=255)                                                                 cTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCgg                                      <  1:180071/139‑1 (MQ=255)                                                                   ggTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGc                                    <  1:145893/139‑1 (MQ=255)                                                                          gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCa                                                                                                           <  2:498821/61‑1 (MQ=255)                                                                          gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCa                                                                                                           >  1:498821/1‑61 (MQ=255)                                                                              aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGc                                                                                            >  1:1043198/1‑72 (MQ=255)                                                                              aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGc                                                                                            <  2:1043198/72‑1 (MQ=255)                                                                              aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGc                                    >  2:253062/1‑128 (MQ=255)                                                                              aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGc                                    <  1:253062/128‑1 (MQ=255)                                                                                  cagccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGta                                 <  1:560570/127‑1 (MQ=255)                                                                                  cagccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGta                                 >  2:560570/1‑127 (MQ=255)                                                                                  cagccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAAcc                     >  2:862161/1‑139 (MQ=255)                                                                                    gccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATGCCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgc                   >  2:842505/1‑139 (MQ=255)                                                                                            tAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGTACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCgccaatgcca           <  1:1235026/139‑1 (MQ=255)                                                                                                   accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCa    >  2:466592/1‑139 (MQ=255)                                                                                                     cacaATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCact  >  2:56972/1‑139 (MQ=255)                                                                                                               ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         >  2:326638/1‑90 (MQ=255)                                                                                                               ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         <  1:326638/90‑1 (MQ=255)                                                                                                                gCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         >  2:113302/1‑89 (MQ=255)                                                                                                                gCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                         <  1:113302/89‑1 (MQ=255)                                                                                                                                |                                                                                                                CCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACT  >  NC_000913/2291846‑2292084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑