Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,228 C→T *274* (TAG→TAA yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2280CT100.0% 101.1 / NA 32*274* (TAG→TAAyfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/18);  total (14/18)

GGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCC  >  NC_000913/2449095‑2449363
                                                                                                                                     |                                                                                                                                       
ggTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACaa                                                                                                                                    <  2:681443/139‑1 (MQ=255)
                              gTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGt                                                                                                      >  2:987102/1‑139 (MQ=255)
                                  aaGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGaa                                                                                                  <  1:550283/139‑1 (MQ=255)
                                       tGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCTGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                             <  2:1111339/139‑1 (MQ=255)
                                          cTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                          <  1:446191/139‑1 (MQ=255)
                                              cgccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGc                                                                                      <  2:712981/139‑1 (MQ=255)
                                                        gCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTa                                                                                                                         >  2:262271/1‑94 (MQ=255)
                                                        gCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTa                                                                                                                         <  1:262271/94‑1 (MQ=255)
                                                                   ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgc                                                                 >  1:428928/1‑139 (MQ=255)
                                                                             tGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTc                                                                                                                             <  2:576627/69‑1 (MQ=255)
                                                                             tGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTc                                                                                                                             >  1:576627/1‑69 (MQ=255)
                                                                                  cAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCgtgt                                                  <  2:428928/139‑1 (MQ=255)
                                                                                            tAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                        <  1:987102/139‑1 (MQ=255)
                                                                                            tAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                        <  1:561181/139‑1 (MQ=255)
                                                                                              aTGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                          >  2:1258074/1‑119 (MQ=255)
                                                                                              aTGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                          <  1:1258074/119‑1 (MQ=255)
                                                                                               tGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTa                                                                          <  2:970626/102‑1 (MQ=255)
                                                                                               tGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTa                                                                          >  1:970626/1‑102 (MQ=255)
                                                                                                     gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGt                                                                                                                          <  2:1099744/48‑1 (MQ=255)
                                                                                                     gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGt                                                                                                                          >  1:1099744/1‑48 (MQ=255)
                                                                                                     gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                  >  1:849718/1‑104 (MQ=255)
                                                                                                     gggCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                  <  2:849718/104‑1 (MQ=255)
                                                                                                          aaTGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGgtgt                          >  2:293974/1‑139 (MQ=255)
                                                                                                                 ctgcctgGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTtata                                <  1:1172706/126‑1 (MQ=255)
                                                                                                                 ctgcctgGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGCtata                                >  2:1172706/1‑126 (MQ=255)
                                                                                                                         tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCt                                                                                        <  2:713364/62‑1 (MQ=255)
                                                                                                                         tgtATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCt                                                                                        >  1:713364/1‑62 (MQ=255)
                                                                                                                             tCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCAATTGGCTGCATACTGCTGGTATCAAcacgca                                                                <  2:943941/82‑1 (MQ=255)
                                                                                                                             tCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCAATTGGCTGCATACTGCTGGTATCAAcacgca                                                                >  1:943941/1‑82 (MQ=255)
                                                                                                                              cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                                >  1:1193937/1‑81 (MQ=255)
                                                                                                                              cTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                                <  2:1193937/81‑1 (MQ=255)
                                                                                                                                  gggTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTcc  >  2:434784/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCC  >  NC_000913/2449095‑2449363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: