Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 206,315 T→C V397A (GTC→GCC)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913206,3150TC100.0% 234.4 / NA 65V397A (GTC→GCC) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/38);  total (27/38)

TAACGGCGTACCGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGTCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGAT  >  NC_000913/206184‑206450
                                                                                                                                   |                                                                                                                                       
tAACGGCGTACCGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATg                                                                                                                                  <  1:495474/139‑1 (MQ=255)
      cGTACCGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGAc                                                                                                                            >  2:946737/1‑139 (MQ=255)
      cGTACCGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGAc                                                                                                                            >  1:626278/1‑139 (MQ=255)
        tACCGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACtt                                                                                                                          >  2:478389/1‑139 (MQ=255)
        tACCGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGCTTCCTTAACCCGGAACGTGCCTCCATGCCTGACtt                                                                                                                          >  1:780538/1‑139 (MQ=255)
          ccGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCg                                                                                                                        <  2:768849/139‑1 (MQ=255)
          ccGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCg                                                                                                                        >  1:660240/1‑139 (MQ=255)
               aGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGtt                                                                                                                   <  1:1035715/139‑1 (MQ=255)
               aGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGtt                                                                                                                   <  2:762664/139‑1 (MQ=255)
               aGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGtt                                                                                                                   <  1:182544/139‑1 (MQ=255)
                 ggCCAGGCCGTGGCTCCGGTGCGTGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGa                                                                                                                 <  2:1115081/139‑1 (MQ=255)
                 ggCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGa                                                                                                                 <  1:1230552/139‑1 (MQ=255)
                  gCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGAc                                                                                                                >  1:292560/1‑139 (MQ=255)
                  gCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGAc                                                                                                                <  2:626278/139‑1 (MQ=255)
                  gCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGAc                                                                                                                <  1:49313/139‑1 (MQ=255)
                    cAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              <  2:1241947/139‑1 (MQ=255)
                    cAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              <  2:780538/139‑1 (MQ=255)
                    cAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              <  1:478389/139‑1 (MQ=255)
                    cAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              <  1:1283124/139‑1 (MQ=255)
                      ggCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGa                                                                                                                 <  1:209029/134‑1 (MQ=255)
                      ggCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGa                                                                                                                 >  2:209029/1‑134 (MQ=255)
                       gCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACt                                                                                                                           >  2:596469/1‑123 (MQ=255)
                       gCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACt                                                                                                                           <  1:596469/123‑1 (MQ=255)
                           tGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATg                                                                                                       >  2:1257972/1‑139 (MQ=255)
                            ggCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATgg                                                                                                      >  2:165226/1‑139 (MQ=255)
                            ggCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCACCATGCCTGACTTCGACGTTGACTTCt                                                                                                            >  1:145886/1‑133 (MQ=255)
                            ggCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCACCATGCCTGACTTCGACGTTGACTTCt                                                                                                            <  2:145886/133‑1 (MQ=255)
                             gCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGga                                                                                                     <  1:989893/139‑1 (MQ=255)
                                                    gCCTACGCGCTGTAAATCTCCGGCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAGCCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGc                                                                              <  2:292560/139‑1 (MQ=255)
                                                    gCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGAc                                                                                                                      <  1:295429/99‑1 (MQ=255)
                                                    gCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGAc                                                                                                                      >  2:295429/1‑99 (MQ=255)
                                                         cgcgCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              <  1:41923/102‑1 (MQ=255)
                                                         cgcgCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              >  2:41923/1‑102 (MQ=255)
                                                         cgcgCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGtt                                                                                     >  1:783700/1‑127 (MQ=255)
                                                         cgcgCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGtt                                                                                     <  2:783700/127‑1 (MQ=255)
                                                             cTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCgg                                                                     <  1:946737/139‑1 (MQ=255)
                                                                aaaaTCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACa                                                                  >  1:509660/1‑139 (MQ=255)
                                                                    tCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTa                                                              <  1:1257972/139‑1 (MQ=255)
                                                                    tCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTa                                                              <  1:165226/139‑1 (MQ=255)
                                                                    tCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTa                                                              <  2:509660/139‑1 (MQ=255)
                                                                       ccGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACgg                                                           <  2:176860/139‑1 (MQ=255)
                                                                          aCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCATGTTATCGAGCACGTAGCGGACATGTACGGTCg                                                        <  2:6444/139‑1 (MQ=255)
                                                                          aCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTa                                                              <  1:1047465/133‑1 (MQ=255)
                                                                          aCCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTa                                                              >  2:1047465/1‑133 (MQ=255)
                                                                             tCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGa                                                     <  1:207542/139‑1 (MQ=255)
                                                                                aTCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCgg                                                                     >  2:8624/1‑120 (MQ=255)
                                                                                aTCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCgg                                                                     <  1:8624/120‑1 (MQ=255)
                                                                                 tCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCg                                                 <  2:1077681/139‑1 (MQ=255)
                                                                                               aCCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATc                                                                                          >  2:240098/1‑84 (MQ=255)
                                                                                               aCCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATc                                                                                          <  1:240098/84‑1 (MQ=255)
                                                                                                ccTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACg                                                                                                                     >  2:545968/1‑56 (MQ=255)
                                                                                                ccTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACg                                                                                                                     <  1:545968/56‑1 (MQ=255)
                                                                                                      gTTCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTa                                                                         >  2:1068177/1‑94 (MQ=255)
                                                                                                      gTTCGAACGTTTCCTGAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTa                                                                         <  1:1068177/94‑1 (MQ=255)
                                                                                                        tCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATTGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCAcc                               <  1:693179/134‑1 (MQ=255)
                                                                                                        tCGAACGTTTCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCAcc                               >  2:693179/1‑134 (MQ=255)
                                                                                                                 tCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGt                                                                          <  2:476413/82‑1 (MQ=255)
                                                                                                                 tCCTTAACCCGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGt                                                                          >  1:476413/1‑82 (MQ=255)
                                                                                                                          cGGAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGc        >  1:1261462/1‑139 (MQ=255)
                                                                                                                           ggAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGatcatc                                  <  2:1154670/112‑1 (MQ=255)
                                                                                                                           ggAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGatcatc                                  >  1:1154670/1‑112 (MQ=255)
                                                                                                                            gAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              <  1:210867/35‑1 (MQ=255)
                                                                                                                            gAACGTGCCTCCATGCCTGACTTCGACGTTGACtt                                                                                                              >  2:210867/1‑35 (MQ=255)
                                                                                                                            gAACGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCgg      >  2:190451/1‑139 (MQ=255)
                                                                                                                             aaCGTGCCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGt     >  1:1171064/1‑139 (MQ=255)
                                                                                                                                gtgcCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGAt  <  1:190451/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TAACGGCGTACCGGTAGGGCCAGGCCGTGGCTCCGGTGCGGGTTCACTGGTGGCCTACGCGCTGAAAATCACCGACCTCGATCCGCTGGAATTTGACCTGCTGTTCGAACGTTTCCTTAACCCGGAACGTGTCTCCATGCCTGACTTCGACGTTGACTTCTGTATGGAGAAACGCGATCAGGTTATCGAGCACGTAGCGGACATGTACGGTCGTGATGCGGTATCGCAGATCATCACCTTCGGTACAATGGCGGCGAAAGCGGTGAT  >  NC_000913/206184‑206450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑