Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,475,251 T→C Y90H (TAT→CAT)  yfdR → CPS‑53 (KpLE1) prophage, conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,475,2510TC100.0% 173.7 / NA 49Y90H (TAT→CAT) yfdRCPS‑53 (KpLE1) prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/24);  total (25/24)

TGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCA  >  NC_000913/2475121‑2475344
                                                                                                                                  |                                                                                             
tgtCGCCCAACATGCGGTGCTTTGCAGCAAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATGAACgg                                                                                       >  1:803717/1‑139 (MQ=255)
  tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACTCCTTCTTCCTGACCATAAACg                                                                                        >  1:1047379/1‑136 (MQ=255)
  tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACTCCTTCTTCCTGACCATAAACg                                                                                        <  2:1047379/136‑1 (MQ=255)
  tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGAt                                                                                     >  2:926758/1‑139 (MQ=255)
     cccAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGga                                                                                  >  1:739567/1‑139 (MQ=255)
            tGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGaaaaa                                                                           <  2:739567/139‑1 (MQ=255)
                   cTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg                                                                       <  2:401431/136‑1 (MQ=255)
                   cTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg                                                                       >  1:401431/1‑136 (MQ=255)
                                                 gCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcctc                                      <  1:292659/139‑1 (MQ=255)
                                                   ttttGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg                                                                                    >  2:164244/1‑91 (MQ=255)
                                                   ttttGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg                                                                                    <  1:164244/91‑1 (MQ=255)
                                                           cATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg                            <  1:62517/139‑1 (MQ=255)
                                                           cATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg                            <  1:926758/139‑1 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa                                                                              <  1:432045/76‑1 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa                                                                              >  2:432045/1‑76 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAAt                                                                         <  2:712918/81‑1 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAAt                                                                         >  1:712918/1‑81 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCGGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                          <  1:309718/128‑1 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct                                       >  1:341899/1‑115 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct                                       <  1:927195/115‑1 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct                                       <  2:341899/115‑1 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct                                       >  2:927195/1‑115 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGaaa               <  1:672229/139‑1 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGaaa               >  1:524761/1‑139 (MQ=255)
                                                                        tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCAATGCAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                          >  2:309718/1‑128 (MQ=255)
                                                                                  gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGa                                                                      <  1:1074038/74‑1 (MQ=255)
                                                                                  gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGa                                                                      >  2:1074038/1‑74 (MQ=255)
                                                                                  gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTgag                                                      <  2:1066335/90‑1 (MQ=255)
                                                                                  gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTgag                                                      >  1:1066335/1‑90 (MQ=255)
                                                                                  gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGAtc     >  1:997671/1‑139 (MQ=255)
                                                                                     tATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCa  >  1:7086/1‑139 (MQ=255)
                                                                                             ggACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAc                                         <  1:892817/92‑1 (MQ=255)
                                                                                             ggACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAc                                         >  2:892817/1‑92 (MQ=255)
                                                                                              gACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg                                                                                    >  1:788889/1‑48 (MQ=255)
                                                                                              gACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg                                                                                    <  2:788889/48‑1 (MQ=255)
                                                                                               aCATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGga                                                                                  <  1:1162151/49‑1 (MQ=255)
                                                                                               aCATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGga                                                                                  >  2:1162151/1‑49 (MQ=255)
                                                                                                   ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                           >  1:565914/1‑84 (MQ=255)
                                                                                                   ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                           <  2:565914/84‑1 (MQ=255)
                                                                                                    cccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                           <  1:643516/83‑1 (MQ=255)
                                                                                                    cccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt                                           >  2:643516/1‑83 (MQ=255)
                                                                                                             cTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa                                                                              <  1:218428/39‑1 (MQ=255)
                                                                                                             cTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa                                                                              >  2:218428/1‑39 (MQ=38)
                                                                                                             cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg                            >  2:1227150/1‑89 (MQ=255)
                                                                                                             cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg                            <  1:1227150/89‑1 (MQ=255)
                                                                                                                     ccTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATAc                                                <  1:733675/61‑1 (MQ=255)
                                                                                                                     ccTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATAc                                                >  2:733675/1‑61 (MQ=255)
                                                                                                                        tcttcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                          >  2:441625/1‑80 (MQ=255)
                                                                                                                        tcttcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa                          <  1:441625/80‑1 (MQ=255)
                                                                                                                                  |                                                                                             
TGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCA  >  NC_000913/2475121‑2475344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: