Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 220,239 C→T L230L (CTG→CTA)  metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913220,2390CT100.0% 163.5 / NA 51L230L (CTG→CTA) metQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/28);  total (23/28)

AGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAG  >  NC_000913/220103‑220360
                                                                                                                                        |                                                                                                                         
aGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAg                                                                                                                         >  2:1004875/1‑139 (MQ=255)
 gCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAgg                                                                                                                        >  2:1132593/1‑139 (MQ=255)
  ccTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGt                                                                                                                       <  1:625162/139‑1 (MQ=255)
       aaaTTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacg                                                                                                                  <  2:464390/139‑1 (MQ=255)
             ccAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                            <  1:1132593/139‑1 (MQ=255)
             ccAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                            <  1:27579/139‑1 (MQ=255)
                gCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGAc                                                                                                         >  2:1123434/1‑139 (MQ=255)
                        cAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATc                                                                                                 <  2:263404/139‑1 (MQ=255)
                              cgccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGttt                                                                                                                     >  1:1238038/1‑113 (MQ=255)
                              cgccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGttt                                                                                                                     <  2:1238038/113‑1 (MQ=255)
                                        cacTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTta                                                                                                                    >  2:597386/1‑104 (MQ=255)
                                        cacTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTta                                                                                                                    <  1:597386/104‑1 (MQ=255)
                                             tgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCttt                                                                            >  1:216840/1‑139 (MQ=255)
                                             tgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCttt                                                                            >  2:258191/1‑139 (MQ=255)
                                                         cGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGttt                                                                                                                     >  1:879184/1‑86 (MQ=255)
                                                         cGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGttt                                                                                                                     <  2:879184/86‑1 (MQ=255)
                                                                 tCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                            >  1:27726/1‑87 (MQ=255)
                                                                 tCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                            <  2:27726/87‑1 (MQ=255)
                                                                  cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTCCGGGCACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                       >  1:763098/1‑139 (MQ=255)
                                                                  cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                       >  1:907061/1‑139 (MQ=255)
                                                                         cTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCa                                                <  1:1004875/139‑1 (MQ=255)
                                                                            aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATAc                                                                                   >  2:845943/1‑101 (MQ=255)
                                                                            aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATAc                                                                                   <  1:845943/101‑1 (MQ=255)
                                                                            aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                                             >  1:1029744/1‑139 (MQ=255)
                                                                            aTAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAg                                             <  2:1016807/139‑1 (MQ=255)
                                                                             tAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                            <  1:258191/139‑1 (MQ=255)
                                                                             tAACCCTGGACGAATTTCTTCACGTTCTTGGCATCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAAGCCAATCTGGCTGGCATAgg                                            <  2:907061/139‑1 (MQ=255)
                                                                                    ggACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                      >  2:63168/1‑122 (MQ=255)
                                                                                    ggACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                      <  1:63168/122‑1 (MQ=255)
                                                                                     gACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTg                                    >  1:738144/1‑139 (MQ=255)
                                                                                      aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGa                                                                                                          <  1:312145/68‑1 (MQ=255)
                                                                                      aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGa                                                                                                          >  2:312145/1‑68 (MQ=255)
                                                                                      aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCATCACGAGTCACGATTAGGTTTACGTACGGCGACTCCTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCCGGCATAgtatgtatttc                                   >  2:637532/1‑129 (MQ=255)
                                                                                       cGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGAt                                  <  2:216840/139‑1 (MQ=255)
                                                                                                    gTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAAt                                                          <  2:461660/102‑1 (MQ=255)
                                                                                                    gTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAAt                                                          >  1:461660/1‑102 (MQ=255)
                                                                                                             ggcTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCCACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg            <  2:763098/137‑1 (MQ=255)
                                                                                                             gTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg            <  2:466775/139‑1 (MQ=255)
                                                                                                             gTCTTTGTTATCATCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg            <  2:652868/139‑1 (MQ=255)
                                                                                                               cTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGcgtc          <  2:1029744/139‑1 (MQ=255)
                                                                                                                tttGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                      >  2:205852/1‑94 (MQ=255)
                                                                                                                tttGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                      <  1:205852/94‑1 (MQ=255)
                                                                                                                       tCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCagag  <  1:977474/139‑1 (MQ=255)
                                                                                                                           cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                      >  1:464755/1‑83 (MQ=255)
                                                                                                                           cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                      <  2:464755/83‑1 (MQ=255)
                                                                                                                           cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCAGAGTCAGGCCAATCTGGCTGGCATAggtg                                          >  2:1174476/1‑95 (MQ=255)
                                                                                                                           cACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCAGAGTCAGGCCAATCTGGCTGGCATAggtg                                          <  1:1174476/95‑1 (MQ=255)
                                                                                                                                   aCGATTAGGTTTACGTACGGGGACTCTTTATCTTcaac                                                                                           >  1:321159/1‑38 (MQ=255)
                                                                                                                                   aCGATTAGGTTTACGTACGGGGACTCTTTATCTTcaac                                                                                           <  2:321159/38‑1 (MQ=255)
                                                                                                                                   aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                 <  1:774789/80‑1 (MQ=255)
                                                                                                                                   aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                 >  2:774789/1‑80 (MQ=255)
                                                                                                                                        |                                                                                                                         
AGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAG  >  NC_000913/220103‑220360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑