Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,015	G→A	*427* (TAG→TAA)	gabT →	4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,015	0	G	A	100.0%	133.0 / NA	43	*427* (TAG→TAA)	gabT	4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/17);  total (26/17)

TAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGG  >  NC_000913/2793880‑2794141                                                                                                                                        |                                                                                                                               tAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAacgc                                                                                                                             <  2:212881/139‑1 (MQ=255)       ccTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTcc                                                                                                                       >  1:227991/1‑139 (MQ=255)                         cGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAGGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCt                                                                                                     >  1:892685/1‑139 (MQ=255)                                           gctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCt                                                                                   >  2:723493/1‑139 (MQ=255)                                            ctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTa                                                                                  <  1:723493/139‑1 (MQ=255)                                                         tACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTa                                                                                  <  2:415896/126‑1 (MQ=255)                                                         tACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTa                                                                                  >  1:415896/1‑126 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCGGCCAGTGTTTTGATGAGGCGAAGCATTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                     >  2:5611/1‑134 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGAc                                                                                                        <  2:1094287/99‑1 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGAc                                                                                                        >  1:1094287/1‑99 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                <  2:268341/123‑1 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                                                >  1:268341/1‑123 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                     <  2:453332/134‑1 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                     >  1:453332/1‑134 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGt                                                                     <  1:5611/134‑1 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:1132114/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:1132572/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:787492/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGAt                                                                >  2:980830/1‑139 (MQ=255)                                                              cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGACCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATGCAGGTCGGAt                                                                >  2:902952/1‑139 (MQ=255)                                                               tCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGTCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCATGTCGGATa                                                               >  1:767599/1‑139 (MQ=255)                                                                 aCCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAg                                                             >  1:1111011/1‑139 (MQ=255)                                                                           aCGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGc                                                                                                      <  2:467989/88‑1 (MQ=255)                                                                           aCGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGc                                                                                                      >  1:467989/1‑88 (MQ=255)                                                                                    tCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGa                                          <  1:1132114/139‑1 (MQ=255)                                                                                      cGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGCCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACCGGGATCCAGGTCGGATAAGGCGTTTTCGCCGCATCCGACa                                        <  1:787492/139‑1 (MQ=255)                                                                                        tCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAAt                                      >  1:208186/1‑139 (MQ=255)                                                                                                tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATg                                                                                                                   <  1:1062333/54‑1 (MQ=255)                                                                                                tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATg                                                                                                                   >  2:1062333/1‑54 (MQ=255)                                                                                                        atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga                      >  1:104362/1‑139 (MQ=255)                                                                                                         tcaGCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                     <  2:1111011/139‑1 (MQ=255)                                                                                                            gCCAGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaagaa                  >  1:1237304/1‑139 (MQ=255)                                                                                                               aGTGTTTTGATGAGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATc               <  2:104362/139‑1 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGa                                                                                                         >  1:630338/1‑37 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGa                                                                                                         <  2:630338/37‑1 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaag                    <  2:400881/122‑1 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaag                    >  1:400881/1‑122 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga                      >  2:213688/1‑120 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga                      <  1:213688/120‑1 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTgg       <  1:697596/135‑1 (MQ=255)                                                                                                                           aGGCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTgg       >  2:697596/1‑135 (MQ=255)                                                                                                                            ggCGAAGCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCggg  >  2:492061/1‑139 (MQ=255)                                                                                                                                  gCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGcc     >  1:929896/1‑130 (MQ=255)                                                                                                                                  gCAGTAACGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTAAGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGcc     <  2:929896/130‑1 (MQ=255)                                                                                                                                        |                                                                                                                               TAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGG  >  NC_000913/2793880‑2794141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑