Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,337	C→T	*150* (TAG→TAA)	ygaU ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,337	0	C	T	100.0%	159.6 / NA	50	*150* (TAG→TAA)	ygaU	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/24);  total (26/24)

CACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCA  >  NC_000913/2796211‑2796467                                                                                                                               |                                                                                                                                   cacGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGa                                                                                                                        <  2:743583/139‑1 (MQ=255)          cACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAac                                                                                                               >  2:1056336/1‑139 (MQ=255)              aGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACtt                                                                                                           >  2:525856/1‑139 (MQ=255)               gACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTg                                                                                                          >  1:132210/1‑139 (MQ=255)                aCCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGc                                                                                                         <  1:228350/139‑1 (MQ=255)                    tGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCg                                                                                                     >  1:236326/1‑139 (MQ=255)                          cggcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGccc                                                                                                       >  2:368321/1‑131 (MQ=255)                          cggcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGccc                                                                                                       <  1:368321/131‑1 (MQ=255)                                               ccGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGa                                                                                                                        <  1:662197/93‑1 (MQ=255)                                               ccGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGa                                                                                                                        >  2:662197/1‑93 (MQ=255)                                               ccGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                          <  2:241245/139‑1 (MQ=255)                                                       gcCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTa                                                                  >  1:172622/1‑139 (MQ=255)                                                           gTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCg                                                              <  2:158390/139‑1 (MQ=255)                                                                 tAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGccc                                                                                                       <  1:313589/92‑1 (MQ=255)                                                                 tAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGccc                                                                                                       >  2:313589/1‑92 (MQ=255)                                                                   aTGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACg                                                                                                                   <  2:749047/78‑1 (MQ=255)                                                                   aTGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACg                                                                                                                   >  1:749047/1‑78 (MQ=255)                                                                        ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                          >  1:1270566/1‑114 (MQ=255)                                                                        ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                          <  2:1270566/114‑1 (MQ=255)                                                                        ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAtttt                                                 >  2:21988/1‑139 (MQ=255)                                                                         cAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                                <  1:799507/139‑1 (MQ=255)                                                                         cAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                                <  2:172622/139‑1 (MQ=255)                                                                          aGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                          <  1:976594/112‑1 (MQ=255)                                                                          aGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                          >  2:976594/1‑112 (MQ=255)                                                                              tttACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATATTAACCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTa                                           <  2:236326/139‑1 (MQ=255)                                                                                     aCGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                                >  2:772643/1‑127 (MQ=255)                                                                                     aCGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                                <  1:772643/127‑1 (MQ=255)                                                                                      cGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGcc                                                                                                        <  2:1177229/70‑1 (MQ=255)                                                                                      cGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGcc                                                                                                        >  1:1177229/1‑70 (MQ=255)                                                                                      cGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAg                                   <  2:132210/139‑1 (MQ=255)                                                                                            ttCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTAc                             >  2:900565/1‑139 (MQ=255)                                                                                            ttCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTAc                             >  1:1214351/1‑139 (MQ=255)                                                                                                 aTTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGc                                  <  1:567634/129‑1 (MQ=255)                                                                                                 aTTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGc                                  >  2:567634/1‑129 (MQ=255)                                                                                                 aTTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAg                        >  1:377381/1‑139 (MQ=255)                                                                                                  ttATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGa                       <  1:21988/139‑1 (MQ=255)                                                                                                    aTCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                                >  2:830165/1‑128 (MQ=255)                                                                                                    aTCCAGCAGGCGATGGCTGGCAACTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                                <  1:830165/128‑1 (MQ=255)                                                                                                     tCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCt                    >  2:579392/1‑139 (MQ=255)                                                                                                       cagcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGAc                      >  2:60015/1‑135 (MQ=255)                                                                                                       cagcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGAc                      <  1:60015/135‑1 (MQ=255)                                                                                                               gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCggg                                                                                    <  2:688554/65‑1 (MQ=255)                                                                                                               gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCggg                                                                                    >  1:688554/1‑65 (MQ=255)                                                                                                               gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCggg                                                                                    >  2:1148563/1‑65 (MQ=255)                                                                                                               gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCggg                                                                                    <  1:1148563/65‑1 (MQ=255)                                                                                                                 tggctggcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGt                                            >  2:659106/1‑103 (MQ=255)                                                                                                                 tggctggcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGt                                            <  1:659106/103‑1 (MQ=255)                                                                                                                       tcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTa                                           <  1:384721/97‑1 (MQ=255)                                                                                                                       gcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTa                                           >  2:384721/1‑98 (MQ=255)                                                                                                                       gcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCa  >  2:129556/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                   CACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCA  >  NC_000913/2796211‑2796467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑