| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,893,928 | G→A | *424* (TAG→TAA) A4T (GCC→ACC) |
ygcN → ygcO → |
putative oxidoreductase putative 4Fe‑4S cluster‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,893,928 | 0 | G | A | 96.4% | 162.1 / ‑1.8 | 56 | *424* (TAG→TAA) A4T (GCC→ACC) | ygcN ygcO | putative oxidoreductase putative 4Fe‑4S cluster‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (31/23); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
GGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAA > NC_000913/2893793‑2894055 | ggATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAAccc > 1:197855/1‑139 (MQ=255)ggATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAAccc > 1:336860/1‑139 (MQ=255) gATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCg > 1:1213556/1‑139 (MQ=255) cccGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGTCATCATTTCCGTCGTCACGGCCTGTGTCTTCTGCCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAAtctc > 2:1023379/1‑139 (MQ=255) gcgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTgg > 2:171101/1‑139 (MQ=255) gATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGgcg < 2:197855/139‑1 (MQ=255) gggTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGt > 1:1054685/1‑128 (MQ=255) gggTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGt < 2:1054685/128‑1 (MQ=255) gATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGca > 1:729946/1‑139 (MQ=255) tCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctg < 2:386298‑M1/139‑14 (MQ=255) tCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggc < 2:450543‑M1/137‑12 (MQ=255) tCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggc > 1:450543‑M1/1‑126 (MQ=255) cgcCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttga > 1:964816‑M1/1‑116 (MQ=255) aTTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccaga > 2:1002141‑M1/1‑108 (MQ=255) tttACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgag < 1:221879‑M1/120‑25 (MQ=255) tttACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgag > 2:221879‑M1/1‑96 (MQ=255) cgtcgtCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAAccc < 1:25328/63‑1 (MQ=255) cgtcgtCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAAccc > 2:25328/1‑63 (MQ=255) gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac > 1:346695‑M1/1‑88 (MQ=255) gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac > 1:485914‑M1/1‑88 (MQ=255) gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac > 1:1210304‑M1/1‑88 (MQ=255) ccTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtc > 2:172545‑M1/1‑81 (MQ=255) tgtgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccg > 1:1006297‑M1/1‑79 (MQ=255) tgtgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcag > 2:1233724‑M1/1‑79 (MQ=255) tgtgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcag < 1:1233724‑M1/116‑38 (MQ=255) gCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcag < 2:1006297‑M1/139‑65 (MQ=255) tCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa > 1:1079168‑M1/1‑72 (MQ=255) tCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa < 2:1079168‑M1/111‑40 (MQ=255) ggcgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttt > 1:976818‑M1/1‑69 (MQ=255) ggcgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacgg < 1:940833‑M1/111‑43 (MQ=255) ggcgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacgg > 2:940833‑M1/1‑69 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttt > 1:208711‑M1/1‑67 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttt > 2:192470‑M1/1‑67 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttt > 1:281784‑M1/1‑67 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtctttt < 1:299890‑M1/138‑72 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtctttt > 2:299890‑M1/1‑67 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccgg < 1:420123‑M1/128‑62 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccgg > 2:420123‑M1/1‑67 (MQ=255) ggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttc > 2:1159721‑M1/1‑65 (MQ=255) gcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcg < 1:172545‑M1/139‑76 (MQ=255) cgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgc < 2:485914‑M1/139‑77 (MQ=255) gTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCt < 2:729946/139‑1 (MQ=255) ttATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcaca < 2:208711‑M1/139‑82 (MQ=255) tATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacac < 2:964816‑M1/139‑83 (MQ=255) tATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacac < 1:171101‑M1/139‑83 (MQ=255) cAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg < 1:132350‑M1/139‑86 (MQ=255) cAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg < 2:976818‑M1/139‑86 (MQ=255) gTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa < 1:15103‑M1/88‑40 (MQ=255) gTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa > 2:15103‑M1/1‑49 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggta > 1:555839‑M1/1‑48 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggta > 2:647797‑M1/1‑48 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg > 2:395434‑M1/1‑48 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg < 1:395434‑M1/133‑86 (MQ=255) tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaac > 2:1141284‑M1/1‑46 (MQ=255) cGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaactt < 1:647797‑M1/139‑96 (MQ=255) cGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaactt < 2:281784‑M1/139‑96 (MQ=255) | GGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAA > NC_000913/2893793‑2894055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |