Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,915,105	C→T	P17S (CCG→TCG)	barA →	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,915,105	0	C	T	100.0%	176.0 / NA	55	P17S (CCG→TCG)	barA	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

CGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGA  >  NC_000913/2914975‑2915237                                                                                                                                   |                                                                                                                                     cgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTc                                                                                                                              <  1:333974/139‑1 (MQ=255)    tttGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCtt                                                                                                                           <  2:346685/139‑1 (MQ=255)      tGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                         <  2:677288/139‑1 (MQ=255)                     cgcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAg                                                                                                          >  1:553768/1‑139 (MQ=255)                       ccATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTc                                                                                                                              <  1:694960/117‑1 (MQ=255)                       ccATTGGCGAGACTTTCTCAATTTAACAGAGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTc                                                                                                                              >  2:694960/1‑117 (MQ=255)                                       ctcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCa                                                                                        >  2:923408/1‑139 (MQ=255)                                        tcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       >  2:528122/1‑139 (MQ=255)                                          aaTTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCg                                                                                     <  1:528122/139‑1 (MQ=255)                                               aaCAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCtata                                                                                <  1:923408/139‑1 (MQ=255)                                                      tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgac                                                                                                                                  >  2:423204/1‑82 (MQ=255)                                                      tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgac                                                                                                                                  <  1:423204/82‑1 (MQ=255)                                                                  tCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       <  2:825068/113‑1 (MQ=255)                                                                  tCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       >  1:825068/1‑113 (MQ=255)                                                                    ccATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTgg                                                           >  1:1012079/1‑139 (MQ=255)                                                                       tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCtttt                                                                                                  >  2:940170/1‑97 (MQ=255)                                                                       tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCtttt                                                                                                  <  1:940170/97‑1 (MQ=255)                                                                       tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAg                                                        >  1:520803/1‑139 (MQ=255)                                                                       tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAg                                                        >  2:259408/1‑139 (MQ=255)                                                                            gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                                >  2:1037995/1‑62 (MQ=255)                                                                            gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                                <  1:1037995/62‑1 (MQ=255)                                                                              aCTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCgg                                                 >  1:133280/1‑139 (MQ=255)                                                                                    tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       <  2:804357/95‑1 (MQ=255)                                                                                    tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTACTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       >  1:804357/1‑95 (MQ=255)                                                                                         aaCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCatta                                      <  1:259408/139‑1 (MQ=255)                                                                                          aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCattat                                     >  2:977307/1‑139 (MQ=255)                                                                                                 ccTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCtata                                                                                <  2:789142/89‑1 (MQ=255)                                                                                                 ccTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCtata                                                                                >  1:789142/1‑89 (MQ=255)                                                                                                   tGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                   >  1:1224846/1‑36 (MQ=255)                                                                                                   tGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                   <  2:1224846/36‑1 (MQ=255)                                                                                                   tGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCt                            >  1:365848/1‑139 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTTATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                          <  1:596658/75‑1 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGa                                                                                                           <  1:378924/58‑1 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGa                                                                                                           >  2:378924/1‑58 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCtttt                                                                                                  >  2:1092033/1‑67 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCtttt                                                                                                  <  1:1092033/67‑1 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                          >  2:596658/1‑75 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       >  1:1213605/1‑78 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       <  2:1213605/78‑1 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                           <  2:315790/138‑1 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                           >  2:993336/1‑138 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                           >  1:315790/1‑138 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                           <  1:993336/138‑1 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTg                          >  2:374315/1‑139 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTg                          >  1:201462/1‑139 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTCCCAGCATTATTGAGCCGCtt                           >  1:765470/1‑138 (MQ=255)                                                                                                     cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTCAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                           <  2:765470/138‑1 (MQ=255)                                                                                                         cacgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                      <  1:1164765/139‑1 (MQ=255)                                                                                                              atgatgATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTa                 <  2:201462/139‑1 (MQ=255)                                                                                                                gatgatTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACt               <  2:1012079/139‑1 (MQ=255)                                                                                                                    atTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                          <  1:99077/60‑1 (MQ=255)                                                                                                                    atTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                          >  2:99077/1‑60 (MQ=255)                                                                                                                     ttCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAata          <  2:1071285/139‑1 (MQ=255)                                                                                                                      tCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAatat         >  1:985436/1‑139 (MQ=255)                                                                                                                          aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                             <  1:257925/35‑1 (MQ=255)                                                                                                                          aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                             >  2:257925/1‑35 (MQ=255)                                                                                                                           tCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCAt    <  1:374315/139‑1 (MQ=255)                                                                                                                           tCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCAt    <  2:133280/139‑1 (MQ=255)                                                                                                                             cTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGa  <  2:553768/139‑1 (MQ=255)                                                                                                                              tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTa                 <  2:433163/123‑1 (MQ=255)                                                                                                                              tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTa                 >  1:433163/1‑123 (MQ=255)                                                                                                                                   |                                                                                                                                     CGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGA  >  NC_000913/2914975‑2915237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑