Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,962,441 C→T *108* (TAG→TAA ppdC ← putative prepilin peptidase‑dependent protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,962,4410CT100.0% 165.9 / NA 52*108* (TAG→TAAppdCputative prepilin peptidase‑dependent protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

GTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCG  >  NC_000913/2962310‑2962574
                                                                                                                                   |                                                                                                                                     
gTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGAc                                                                                                                                >  2:1132737/1‑139 (MQ=255)
        cACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCggg                                                                                                                        >  2:73773/1‑139 (MQ=255)
             gAATCATCTCTGGTTCGAAAGGCTCGTCCAACCGTTCGCGTTCGACAATAAACTTCATCAACGCTTCCAGCACGTCCAGGCGATTGGAATGGTAGTCACTTAACATAGCGACTCCTGATTACTGACGATTCGGGCAATg                                                                                                                   >  2:27645/1‑139 (MQ=255)
                       tGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGAt                                                                                                                             <  2:657394/119‑1 (MQ=255)
                       tGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGAt                                                                                                                             >  1:657394/1‑119 (MQ=255)
                       tGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTc                                                                                                         >  2:396640/1‑139 (MQ=255)
                        ggTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCa                                                                                                        >  1:345088/1‑139 (MQ=255)
                            cGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAAt                                                                                                                    >  2:503422/1‑123 (MQ=255)
                            cGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAAt                                                                                                                    <  1:503422/123‑1 (MQ=255)
                              aaaGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGc                                                                                                  >  1:1262613/1‑139 (MQ=255)
                                          aGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  2:163610/139‑1 (MQ=255)
                                          aGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  1:73773/139‑1 (MQ=255)
                                                                    cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            >  1:980976/1‑139 (MQ=255)
                                                                    cATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATg                                                            <  2:345088/139‑1 (MQ=255)
                                                                         aCGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGc                                                                                                  >  2:389337/1‑96 (MQ=255)
                                                                         aCGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGc                                                                                                  <  1:389337/96‑1 (MQ=255)
                                                                          cGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGaca                                                      >  1:510655/1‑139 (MQ=255)
                                                                             ttCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACAt                                                   <  1:1072574/139‑1 (MQ=255)
                                                                                     cGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAt                                                                                                       <  2:327691/79‑1 (MQ=255)
                                                                                     cGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCAt                                                                                                       >  1:327691/1‑79 (MQ=255)
                                                                                      gTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGc                                          <  2:982341/139‑1 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  2:312231/1‑139 (MQ=255)
                                                                                       tCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         <  2:1262613/139‑1 (MQ=255)
                                                                                        ccAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGa                                        <  1:396640/139‑1 (MQ=255)
                                                                                        ccAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGa                                        <  2:781208/139‑1 (MQ=255)
                                                                                           gACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTa                                                                          <  1:1217069/102‑1 (MQ=255)
                                                                                           gACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTa                                                                          >  2:1217069/1‑102 (MQ=255)
                                                                                                 tGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTGACGCTGATGCTGACACATCCCGCCTGCGa                                        <  2:746814/130‑1 (MQ=255)
                                                                                                 tGGAATGGTAGACCCTTAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGa                                        >  1:746814/1‑130 (MQ=255)
                                                                                                                ttAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      >  2:383627/1‑69 (MQ=255)
                                                                                                                ttAACATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGcccc                                                                                      <  1:383627/69‑1 (MQ=255)
                                                                                                                  aaCATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         <  1:134890/112‑1 (MQ=255)
                                                                                                                  aaCATAGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  2:134890/1‑112 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCggg                                                                                  >  2:1097552/1‑66 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCggg                                                                                  <  1:1097552/66‑1 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCt                                                                >  1:1275439/1‑84 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCt                                                                <  2:1275439/84‑1 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         <  2:829306/107‑1 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  1:829306/1‑107 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCa         >  2:81424/1‑139 (MQ=255)
                                                                                                                       aGCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCa         <  1:81424/139‑1 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  1:359930/1‑106 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         <  2:359930/106‑1 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAg        >  1:819630/1‑139 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAg        >  1:1212035/1‑139 (MQ=255)
                                                                                                                        gCGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAg        >  1:1160407/1‑139 (MQ=255)
                                                                                                                         cGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAgg       >  2:1104441/1‑139 (MQ=255)
                                                                                                                         cGGCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAgg       <  2:1160407/139‑1 (MQ=255)
                                                                                                                          ggCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         <  1:1132312/104‑1 (MQ=255)
                                                                                                                          ggCTCCTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg                                         >  2:1132312/1‑104 (MQ=255)
                                                                                                                              ccTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCg  >  1:173274/1‑139 (MQ=255)
                                                                                                                              ccTGATTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCg  >  1:951055/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                     
GTACTTTGCACCAGAATCATCTCTGGTTCGAAAGGATCGTCCAGCCGTTCGCGTTCGACAATAAACTCCATCAACGCTTCCAGCACGTCCAGACGATTGGAATGGTAGACCCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCG  >  NC_000913/2962310‑2962574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: