Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	30,616	T→C	P322P (CCT→CCC)	carA →	carbamoyl phosphate synthetase small subunit, glutamine amidotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	30,616	0	T	C	100.0%	196.2 / NA	54	P322P (CCT→CCC)	carA	carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/24);  total (30/24)

GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACA  >  NC_000913/30481‑30732                                                                                                                                        |                                                                                                                     ggcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCa                                                                                                                   >  1:885491/1‑139 (MQ=255)          tGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCgt                                                                                                          >  1:674533/1‑139 (MQ=255)                    gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa                                                                                                >  2:76958/1‑139 (MQ=255)                    gTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa                                                                                                >  2:1057822/1‑139 (MQ=255)                                 ttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                   <  1:952453/139‑1 (MQ=255)                                 ttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa                                                                                   <  1:76958/139‑1 (MQ=255)                                     tCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCgtgt                                                                                                        <  1:1156583/114‑1 (MQ=255)                                     tCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCgtgt                                                                                                        >  2:1156583/1‑114 (MQ=255)                                      caccacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa                                                                              <  2:828056/139‑1 (MQ=255)                                            ggcggcAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                                                        <  1:1012042/139‑1 (MQ=255)                                                   aCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCa                                                                                                                   <  1:49489/89‑1 (MQ=255)                                                   aCCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCACCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCa                                                                                                                   >  2:49489/1‑89 (MQ=255)                                                      aTCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCAt                                                                                                 <  1:1226225/104‑1 (MQ=255)                                                      aTCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCAt                                                                                                 >  2:1226225/1‑104 (MQ=255)                                                      aTCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGc                                                                   <  1:1083629/134‑1 (MQ=255)                                                      aTCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGc                                                                   >  2:1083629/1‑134 (MQ=255)                                                               aaGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAGACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTATGTTACAGGGCATTCATCGCACCGa                                                     <  2:674533/139‑1 (MQ=255)                                                                   tgtgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATaaa                                                 >  2:288757/1‑139 (MQ=255)                                                                     tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCt                                                                                         <  2:1277315/97‑1 (MQ=255)                                                                     tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCt                                                                                         >  1:1277315/1‑97 (MQ=255)                                                                     tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAAcc                                               >  2:311809/1‑139 (MQ=255)                                                                     tgGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAAcc                                               >  1:69145/1‑139 (MQ=255)                                                                      ggAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa                                                                                                >  1:23282/1‑89 (MQ=255)                                                                      ggAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATa                                                                                                <  2:23282/89‑1 (MQ=255)                                                                             aaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCa                                       <  1:311809/139‑1 (MQ=255)                                                                                      aTGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAgg                              >  1:729891/1‑139 (MQ=255)                                                                                          tCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCa                          >  1:660492/1‑139 (MQ=255)                                                                                             ccGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCAccc                       >  1:1032945/1‑139 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCg                                                           <  2:833258/102‑1 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCg                                                           >  1:833258/1‑102 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAgggg                            >  1:576583/1‑133 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAgggg                            <  2:576583/133‑1 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt                      >  1:707366/1‑139 (MQ=255)                                                                                              cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt                      >  1:875486/1‑139 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCa                                       <  2:1168455/120‑1 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCa                                       >  1:1168455/1‑120 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGc                           <  1:54592/132‑1 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGc                           >  2:54592/1‑132 (MQ=255)                                                                                                cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGa                    >  1:366652/1‑139 (MQ=255)                                                                                                   aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa                                                                              >  1:6623/1‑78 (MQ=255)                                                                                                   aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa                                                                              <  2:6623/78‑1 (MQ=255)                                                                                                   aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc                 <  2:1006017/139‑1 (MQ=255)                                                                                                   aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc                 <  1:1057822/139‑1 (MQ=255)                                                                                                     aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAg                                      >  2:459143/1‑116 (MQ=255)                                                                                                     aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAg                                      <  1:459143/116‑1 (MQ=255)                                                                                                     aaCCACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagcca               >  1:49597/1‑139 (MQ=255)                                                                                                        cACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagccagcc            <  2:69145/139‑1 (MQ=255)                                                                                                        cACGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagccagcc            <  2:875486/139‑1 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                                                        >  1:846191/1‑77 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTAc                                                                        <  2:846191/77‑1 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCt          >  2:810855/1‑139 (MQ=255)                                                                                                          cGGTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCt          >  2:255120/1‑139 (MQ=255)                                                                                                           ggTTTTGCGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTg         >  2:352306/1‑139 (MQ=255)                                                                                                                  cGGTGGACGAAGCAACATTACCCGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCcaca  <  1:144105/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                     GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACA  >  NC_000913/30481‑30732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑