Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,373	C→T	*578* (TAG→TAA)	recJ ←	ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,373	0	C	T	100.0%	222.0 / NA	69	*578* (TAG→TAA)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/33);  total (36/33)

CGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTA  >  NC_000913/3036238‑3036502                                                                                                                                        |                                                                                                                                  cGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTaa                                                                                                                                >  2:424958/1‑139 (MQ=255)      ggTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCTATCCACGCTCTTTTTTATAGAGAAGTTGACGTTAAATTgg                                                                                                                           <  1:787380/139‑1 (MQ=255)      ggTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                           <  2:1239283/139‑1 (MQ=255)        tCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGcc                                                                                                                         <  1:737953/139‑1 (MQ=255)         ccTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTg                                                                                                                            <  2:896222/135‑1 (MQ=255)         ccTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTg                                                                                                                            >  1:896222/1‑135 (MQ=255)                    ttattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCga                                                                                                             <  2:231328/139‑1 (MQ=255)                                              ggTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGc                                                                                                                          >  2:535454/1‑100 (MQ=255)                                              ggTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGc                                                                                                                          <  1:535454/100‑1 (MQ=255)                                                tCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCcgcg                                                                                 >  1:205036/1‑139 (MQ=255)                                                        cTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGt                                                                         <  2:289091/139‑1 (MQ=255)                                                              ttGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                           >  2:1080358/1‑83 (MQ=255)                                                              ttGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTgg                                                                                                                           <  1:1080358/83‑1 (MQ=255)                                                              ttGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGatat                                                                   >  1:964387/1‑139 (MQ=255)                                                                   cTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGc                                                              >  1:764060/1‑139 (MQ=255)                                                                      gTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                               <  1:131727/71‑1 (MQ=255)                                                                      gTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTaaa                                                                                                                               >  2:131727/1‑71 (MQ=255)                                                                      gTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAg                                                                                                                       >  1:447861/1‑79 (MQ=255)                                                                      gTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAg                                                                                                                       <  2:447861/79‑1 (MQ=255)                                                                           aaTGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGa                                                                                >  2:613319/1‑113 (MQ=255)                                                                           aaTGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGa                                                                                <  1:613319/113‑1 (MQ=255)                                                                           aaTGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGc                                                      <  1:424958/139‑1 (MQ=255)                                                                                ggTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                              >  1:428643/1‑110 (MQ=255)                                                                                ggTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                >  1:31706/1‑124 (MQ=255)                                                                                ggTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                <  2:31706/124‑1 (MQ=255)                                                                                ggTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGACGCTAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                              <  2:428643/110‑1 (MQ=255)                                                                                 gTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTg                                                >  1:185216/1‑139 (MQ=255)                                                                                        gagCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATGATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGGTATCGAGCTTATAAGCCAGTTGCACTTcg                                         >  1:778375/1‑139 (MQ=255)                                                                                           cGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                      <  2:964387/139‑1 (MQ=255)                                                                                            gggATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                <  1:1289645/112‑1 (MQ=255)                                                                                            gggATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                >  2:1289645/1‑112 (MQ=255)                                                                                            gggATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                      <  2:463221/138‑1 (MQ=255)                                                                                            gggATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                      >  1:463221/1‑138 (MQ=255)                                                                                              gATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTg                                                                                                                            <  2:971669/50‑1 (MQ=255)                                                                                              gATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTg                                                                                                                            >  1:971669/1‑50 (MQ=255)                                                                                              gATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACg                                   >  2:1233213/1‑139 (MQ=255)                                                                                                   tACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                              <  1:1101118/91‑1 (MQ=255)                                                                                                   tACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                                              >  2:1101118/1‑91 (MQ=255)                                                                                                       cAATCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATcc                          <  1:1233213/139‑1 (MQ=255)                                                                                                        aaTCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGc                                                                                                                          >  2:835753/1‑42 (MQ=255)                                                                                                        aaTCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGc                                                                                                                          <  1:835753/42‑1 (MQ=255)                                                                                                         aTCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTTCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCgg                        >  2:927699/1‑139 (MQ=255)                                                                                                          tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                <  2:236088/98‑1 (MQ=255)                                                                                                          tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                >  1:236088/1‑98 (MQ=255)                                                                                                          tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                      >  1:44685/1‑124 (MQ=255)                                                                                                          tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc                                      <  2:44685/124‑1 (MQ=255)                                                                                                          tCCACGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGc                       >  2:1171132/1‑139 (MQ=255)                                                                                                              cGCTCTTTTTTATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCaga                   <  2:764060/139‑1 (MQ=255)                                                                                                                   ttttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCg              <  1:1171132/139‑1 (MQ=255)                                                                                                                    tttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATc                                                                  <  2:19686/86‑1 (MQ=255)                                                                                                                    tttttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATc                                                                  >  1:19686/1‑86 (MQ=255)                                                                                                                       ttATAGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTAt          <  1:927699/139‑1 (MQ=255)                                                                                                                         ataGAGAAGATGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCg        <  2:205036/139‑1 (MQ=255)                                                                                                                             agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                <  2:1119665/79‑1 (MQ=255)                                                                                                                             agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGa                                                                >  1:1119665/1‑79 (MQ=255)                                                                                                                             agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGc                                                      <  1:820521/89‑1 (MQ=255)                                                                                                                             agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGc                                                      >  2:820521/1‑89 (MQ=255)                                                                                                                             agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACAt    >  1:522256/1‑139 (MQ=255)                                                                                                                             agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACAt    >  2:1161932/1‑139 (MQ=255)                                                                                                                             agaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACAt    >  1:89793/1‑139 (MQ=255)                                                                                                                             agaagaTAACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACAt    >  1:791100/1‑139 (MQ=255)                                                                                                                              gaagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGGTATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGTCCAGAGGGCGGTATCGACAtt   <  2:778375/139‑1 (MQ=255)                                                                                                                               aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATaa                                                        >  2:972503/1‑85 (MQ=255)                                                                                                                               aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATaa                                                        <  1:972503/85‑1 (MQ=255)                                                                                                                               aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACtt                                           <  2:40200/98‑1 (MQ=255)                                                                                                                               aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACtt                                           >  1:40200/1‑98 (MQ=255)                                                                                                                               aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTa  >  2:1285708/1‑139 (MQ=255)                                                                                                                               aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTa  >  1:369644/1‑139 (MQ=255)                                                                                                                               aagaTGACGTTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTa  >  2:975565/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                  CGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTATCCGGCCAGAGGGCGGTATCGACATTA  >  NC_000913/3036238‑3036502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑