Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	280,024	C→T	pseudogene (94/174 nt)	insI1 →	IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	280,024	0	C	T	100.0%	97.7 / NA	34	pseudogene (94/174 nt)	insI1	IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)

ACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGC  >  NC_000913/279893‑280156                                                                                                                                    |                                                                                                                                     acCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACATTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAaacaa                                                                                                                               >  2:647317/1‑139 (MQ=255)     tcatACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGa                                                                                                                           <  1:115796/139‑1 (MQ=255)                     tAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGa                                                                                                           >  1:616857/1‑139 (MQ=255)                       aGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTg                                                                                                            >  2:382681/1‑136 (MQ=255)                       aGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTg                                                                                                            <  1:382681/136‑1 (MQ=255)                       aGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAg                                                                                                         >  2:317339/1‑139 (MQ=255)                           ggCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCgag                                                                                                                      >  1:1229300/1‑122 (MQ=255)                           ggCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCgag                                                                                                                      <  2:1229300/122‑1 (MQ=255)                            gcagcaTCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCaa                                                                                                    <  1:647317/139‑1 (MQ=255)                               gcaTCACCGAGAACAGAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAac                                                                                                 >  2:867481/1‑139 (MQ=255)                                   cACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCg                                                                                             <  2:134753/139‑1 (MQ=18)                                      cGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGaaa                                                                                          >  2:597732/1‑139 (MQ=11)                                                                                           cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTAAAGCAGCttttt                                     >  2:1123079/1‑139 (MQ=21)                                                                                                tataCTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAat                                >  2:699489/1‑139 (MQ=255)                                                                                                     tCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                           >  2:668040/1‑139 (MQ=255)                                                                                                      cAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTcc                          <  1:597732/139‑1 (MQ=255)                                                                                                       aaCATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTccc                         >  2:567695/1‑139 (MQ=255)                                                                                                         cATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGt                       >  1:1097730/1‑139 (MQ=255)                                                                                                         cATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGt                       >  2:58954/1‑139 (MQ=255)                                                                                                          aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt                      >  1:241713/1‑139 (MQ=255)                                                                                                          aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt                      <  2:616857/139‑1 (MQ=255)                                                                                                           tGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTtc                     <  2:241713/139‑1 (MQ=255)                                                                                                           tGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTtc                     <  1:699489/139‑1 (MQ=255)                                                                                                                   aTCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt                      <  2:127242/130‑1 (MQ=255)                                                                                                                   aTCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt                      >  1:127242/1‑130 (MQ=255)                                                                                                                       ggTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACagcagc                                          <  1:746816/106‑1 (MQ=17)                                                                                                                       ggTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACagcagc                                          >  2:746816/1‑106 (MQ=17)                                                                                                                       ggTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCg         >  1:134833/1‑139 (MQ=255)                                                                                                                       ggTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCg         >  1:272094/1‑139 (MQ=255)                                                                                                                             tgctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt                      <  2:28092/120‑1 (MQ=255)                                                                                                                             tgctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt                      >  1:28092/1‑120 (MQ=255)                                                                                                                              gctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGc  <  2:134833/139‑1 (MQ=255)                                                                                                                              gctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGc  <  1:71692/139‑1 (MQ=255)                                                                                                                              gctCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGc  >  2:693183/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                     ACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAACCCGTTTCAGC  >  NC_000913/279893‑280156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑