Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,065	G→A	*351* (TAG→TAA)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,065	0	G	A	100.0%	254.1 / NA	78	*351* (TAG→TAA)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/42);  total (36/42)

TCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCT  >  NC_000913/3103930‑3104195                                                                                                                                        |                                                                                                                                   tCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAacgc                                                                                                                                 >  1:677549/1‑139 (MQ=255)  cccGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAAcgcg                                                                                                                                <  1:1089928/139‑1 (MQ=255)          gtcATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGC‑GTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGAt                                                                                                                       >  2:16891/1‑139 (MQ=255)            cATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATa                                                                                                                      <  2:465495/139‑1 (MQ=255)             aTTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaa                                                                                                                     >  2:20683/1‑139 (MQ=255)               tCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAag                                                                                                                   <  1:20683/139‑1 (MQ=255)                   cGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGga                                                                                                               >  1:42632/1‑139 (MQ=255)                    ggCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  1:398894/139‑1 (MQ=255)                    ggCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  2:677549/139‑1 (MQ=255)                     gCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgatg                                                                                                             >  1:1257731/1‑139 (MQ=255)                     gCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgatg                                                                                                             >  2:1169195/1‑139 (MQ=255)                         cATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaaa                                                                                                                    >  2:369354/1‑128 (MQ=255)                         cATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaaa                                                                                                                    <  1:369354/128‑1 (MQ=255)                         cATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGAttt                                                                                                         <  1:672750/139‑1 (MQ=255)                         cATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGAttt                                                                                                         >  1:1020327/1‑139 (MQ=255)                                   ggCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGaa                                                                                               >  2:1252589/1‑139 (MQ=255)                                   ggCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGaa                                                                                               >  1:1033742/1‑139 (MQ=255)                                       aTGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAt                                                                                           <  2:1033742/139‑1 (MQ=255)                                        tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtt                                                                                          >  1:588430/1‑139 (MQ=255)                                        tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCACCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGAAGAACGAtt                                                                                          >  2:260742/1‑139 (MQ=255)                                               tGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  2:42632/139‑1 (MQ=255)                                               tGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  1:1169195/139‑1 (MQ=255)                                                       cTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                           <  2:588430/139‑1 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAag                                                                                                                   <  2:669397/95‑1 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAag                                                                                                                   >  1:669397/1‑95 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAAc                                                                       >  1:1285519/1‑139 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGa                                                                                                                             <  1:467632/84‑1 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGa                                                                                                                             >  2:645821/1‑84 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGa                                                                                                                             >  2:467632/1‑84 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGa                                                                                                                             <  1:645821/84‑1 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGga                                                                                                               <  2:783712/98‑1 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGga                                                                                                               >  1:783712/1‑98 (MQ=255)                                                                aCCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGaa                                                                  >  2:86983/1‑139 (MQ=255)                                                                 ccgccgTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAg                                                                                                    <  1:613771/104‑1 (MQ=255)                                                                 ccgccgTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAg                                                                                                    >  2:613771/1‑104 (MQ=255)                                                                 ccgccgTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAg                                                                 >  1:745799/1‑139 (MQ=255)                                                                           ttGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCCGAACGATTTTTTGTACTTTCCTGCAACg                                                                      >  1:221776/1‑124 (MQ=255)                                                                           ttGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACg                                                                      <  2:221776/124‑1 (MQ=255)                                                                                 tAGCGGCTCCCGTGGAGCGTTTGTTACAGC‑GTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGAtttt                                                <  1:16891/139‑1 (MQ=255)                                                                                  aGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGAtttt                                                <  1:86983/139‑1 (MQ=255)                                                                                  aGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGAtttt                                                <  2:1257731/139‑1 (MQ=255)                                                                                   gCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTg                                                                                                                              <  1:841944/60‑1 (MQ=255)                                                                                   gCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTg                                                                                                                              >  2:841944/1‑60 (MQ=255)                                                                                   gCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAg                                                                                                                            >  2:1247102/1‑62 (MQ=255)                                                                                   gCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAg                                                                                                                            <  1:1247102/62‑1 (MQ=255)                                                                                   gCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                             <  2:1085607/93‑1 (MQ=255)                                                                                   gCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                             >  1:1085607/1‑93 (MQ=255)                                                                                        tCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAacg                                                                                                                                  <  2:8818/51‑1 (MQ=255)                                                                                                 gCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  2:346602/89‑1 (MQ=255)                                                                                                 gCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   >  1:346602/1‑89 (MQ=255)                                                                                                  cGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  1:144078/88‑1 (MQ=255)                                                                                                  cGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   >  2:144078/1‑88 (MQ=255)                                                                                                  cGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCg                                   >  2:1213036/1‑136 (MQ=255)                                                                                                  cGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCg                                   <  1:1213036/136‑1 (MQ=255)                                                                                                    tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  2:884938/86‑1 (MQ=255)                                                                                                    tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   >  1:884938/1‑86 (MQ=255)                                                                                                         tACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTggg                         <  1:1252589/139‑1 (MQ=255)                                                                                                          aCAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtttttg                                                                                      <  1:1051166/77‑2 (MQ=255)                                                                                                          aCAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtttttg                                                                                      >  2:1051166/1‑76 (MQ=255)                                                                                                               agTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACg                   <  2:677266/139‑1 (MQ=255)                                                                                                                  tACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCAt                >  1:565995/1‑139 (MQ=255)                                                                                                                  tACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCAt                <  1:1118630/139‑1 (MQ=255)                                                                                                                   aCGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCa                                                                         >  1:831342/1‑81 (MQ=255)                                                                                                                   aCGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCa                                                                         <  2:831342/81‑1 (MQ=255)                                                                                                                    cGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCt              <  2:565995/139‑1 (MQ=255)                                                                                                                        cTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATaa          >  1:752848/1‑139 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACGCGTGAGTCGATAAagag                                                                                                                 >  2:239220/1‑35 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACGCGTGAGTCGATAAagag                                                                                                                 <  1:239220/35‑1 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAAc         <  2:745799/139‑1 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAAc         >  1:1159861/1‑139 (MQ=255)                                                                                                                          ggCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACg        <  2:1159861/139‑1 (MQ=255)                                                                                                                            cgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag      <  2:752848/139‑1 (MQ=255)                                                                                                                            cgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag      <  2:1285519/139‑1 (MQ=255)                                                                                                                               gcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCgg                                  <  1:362524/108‑1 (MQ=255)                                                                                                                               gcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCgg                                  >  2:362524/1‑108 (MQ=255)                                                                                                                                ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCAt                >  2:846285/1‑125 (MQ=255)                                                                                                                                ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCAt                <  1:846285/125‑1 (MQ=255)                                                                                                                                ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGAtct  <  1:717114/139‑1 (MQ=255)                                                                                                                                 cGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTc                                                        <  2:225073/84‑1 (MQ=255)                                                                                                                                 cGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTc                                                        >  1:225073/1‑84 (MQ=255)                                                                                                                                        |                                                                                                                                   TCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCT  >  NC_000913/3103930‑3104195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑